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Our CRISPR-generated, fluorescently tagged endogenous Caenorhabditis elegans RHEB-1 and DAF-15\/Raptor are expressed ubiquitously and localize to lysosomes. LET-363\/TOR and DAF-15\/Raptor are required for development beyond the third larval stage (L3). We observed that deletion of RHEB-1 similarly conferred L3 arrest. Unexpectedly, robust RNAi-mediated depletion of TORC1 components caused arrest at stages prior to L3. Accordingly, conditional depletion of endogenous DAF-15\/Raptor in the soma revealed that TORC1 is required at each stage of the life cycle to progress to the next stage. Reversal of DAF-15 depletion permits arrested animals to recover to continue development. Our results are consistent with TORC1 functioning as a developmental checkpoint that governs the decision of the animal to progress through development.<\/jats:p>","DOI":"10.1242\/dev.181727","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T15:19:19Z","timestamp":1581347959000},"source":"Crossref","is-referenced-by-count":32,"title":["The Rheb-TORC1 signaling axis functions as a developmental checkpoint"],"prefix":"10.1242","volume":"147","author":[{"given":"Tam","family":"Duong","sequence":"first","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University , Houston, TX 77030 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal R.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University , Houston, TX 77030 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliot","family":"Ballato","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University , Houston, TX 77030 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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