{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:30:49Z","timestamp":1780918249212,"version":"3.54.1"},"reference-count":64,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM102356"],"award-info":[{"award-number":["GM102356"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Lateral zebrafish hypoblast cells initiate dorsal convergence near mid-gastrulation and exhibit non-polarized morphologies, limited cell-cell contact, and indirect migration trajectories. By late gastrulation mesodermal cells become packed as they engage in planar cell polarity (PCP)-dependent movement. Here, we aimed to understand this transition in cell behavior by examining the relationship between protrusion dynamics and establishment of PCP and directed migration. We found that wild-type cells undergo a reduction in bleb protrusions near late gastrulation accompanied by a VANGL planar cell polarity protein 2 (Vangl2) regulated increase in filopodia number and polarization. Manipulation of blebs is sufficient to interfere with PCP and directed migration. We show that Vangl2, fibronectin, and Cadherin 2 function to suppress blebbing. Vangl2 maintains Ezrin b (Ezrb) protein levels and higher Ezrb activation rescues defective mediolateral cell alignment and migration paths in vangl2 mutant embryos. Transplantation experiments show that loss of vangl2 disrupts protrusion formation cell-autonomously while fibronectin acts non-autonomously. We propose that dorsal convergence requires the coordinated action of Vangl2, Ezrb, and cell adhesion proteins to inhibit blebs and promote polarized actin-rich protrusive activity and PCP.<\/jats:p>","DOI":"10.1242\/dev.182188","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T15:55:12Z","timestamp":1573574112000},"source":"Crossref","is-referenced-by-count":7,"title":["Dorsal convergence of gastrula cells requires a Vangl2 and adhesion protein-dependent change in protrusive activity"],"prefix":"10.1242","author":[{"given":"Dianna J.","family":"Prince","sequence":"first","affiliation":[{"name":"Department of Biology, Middle Tennessee State University, Murfreesboro, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8209-3620","authenticated-orcid":false,"given":"Jason R.","family":"Jessen","sequence":"additional","affiliation":[{"name":"Department of Biology, Middle Tennessee State University, Murfreesboro, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042623010128100_DEV182188C1","doi-asserted-by":"publisher","first-page":"4027","DOI":"10.1242\/jcs.02522","article-title":"Transition from non-motile behaviour to directed migration during early PGC development in zebrafish","volume":"118","author":"Blaser","year":"2005","journal-title":"J. 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