{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:03:20Z","timestamp":1772168600258,"version":"3.50.1"},"reference-count":56,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100011264","name":"FP7 People: Marie-Curie Actions","doi-asserted-by":"crossref","award":["624441"],"award-info":[{"award-number":["624441"]}],"id":[{"id":"10.13039\/100011264","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["N012291"],"award-info":[{"award-number":["N012291"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["N006542"],"award-info":[{"award-number":["N006542"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Thalamocortical axons (TCAs) cross several tissues on their journey to the cortex. Mechanisms must be in place along the route to ensure they connect with their targets in an orderly fashion. The ventral telencephalon acts as an instructive tissue, but the importance of the diencephalon in TCA mapping is unknown. We report that disruption of diencephalic development by Pax6 deletion results in a thalamocortical projection containing mapping errors. We used conditional mutagenesis to test whether these errors are due to the disruption of pioneer projections from prethalamus to thalamus and found that, while this correlates with abnormal TCA fasciculation, it does not induce topographical errors. To test whether the thalamus contains navigational cues for TCAs, we used slice culture transplants and gene expression studies. We found the thalamic environment is instructive for TCA navigation and that the molecular cues Netrin1 and Semaphorin3a are likely to be involved. Our findings indicate that the correct topographic mapping of TCAs onto the cortex requires the order to be established from the earliest stages of their growth by molecular cues in the thalamus itself.<\/jats:p>","DOI":"10.1242\/dev.184523","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T17:45:19Z","timestamp":1592243119000},"source":"Crossref","is-referenced-by-count":9,"title":["The role of the diencephalon in the guidance of thalamocortical axons in mice"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9297-7331","authenticated-orcid":false,"given":"Idoia","family":"Quintana-Urzainqui","sequence":"first","affiliation":[{"name":"Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Hern\u00e1ndez-Malmierca","sequence":"additional","affiliation":[{"name":"Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), 69120 Heidelberg, Germany"},{"name":"Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Clegg","sequence":"additional","affiliation":[{"name":"Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziwen","family":"Li","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Science, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zrinko","family":"Kozi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Price","sequence":"additional","affiliation":[{"name":"Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042623454892600_DEV184523C1","first-page":"255","article-title":"Monosynaptic and polysynaptic activation of pyramidal tract neurons by thalamic stimulation","volume-title":"The Thalamus","author":"Amassian","year":"1966"},{"key":"2021042623454892600_DEV184523C2","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.jneumeth.2005.01.006","article-title":"Free-D: an integrated environment for three-dimensional reconstruction from serial sections","volume":"145","author":"Andrey","year":"2005","journal-title":"J. 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