{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:28:06Z","timestamp":1790512086380,"version":"4.1.0"},"reference-count":77,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"name":"National Institute of Health","award":["NIH R01 AR074526"],"award-info":[{"award-number":["NIH R01 AR074526"]}]},{"DOI":"10.13039\/100004412","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGP0052\/2018"],"award-info":[{"award-number":["RGP0052\/2018"]}],"id":[{"id":"10.13039\/100004412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Satellite cells (SC) are muscle stem cells which can regenerate adult muscles upon injury. Most SC originate from PAX7-positive myogenic precursors set aside during development. While myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced Pluripotent Stem (iPS) cell reporter lines in which fluorescent proteins have been introduced into the PAX7 and MYOG loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPS-derived PAX7-positive myogenic precursors. We show that the PAX7-positive cells generated in culture can produce myofibers and self-renew in vitro and in vivo. Together, we demonstrate that cells exhibiting characteristics of human fetal satellite cells can be produced in vitro from iPS cells, opening interesting avenues for muscular dystrophy cell therapy. This work provides significant insights into the development of the human myogenic lineage.<\/jats:p>","DOI":"10.1242\/dev.187344","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T21:44:37Z","timestamp":1592257477000},"source":"Crossref","is-referenced-by-count":49,"title":["Differentiation of the human PAX7-positive myogenic precursors\/satellite cell lineage <i>in vitro<\/i>"],"prefix":"10.1242","author":[{"given":"Ziad","family":"Al Tanoury","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Universit\u00e9 de Strasbourg, Illkirch Graffenstaden, France"},{"name":"Department of Pathology, Brigham and Women's Hospital, 60 Fenwood road, Boston, Massachusetts, USA"},{"name":"Department of Genetics, Harvard Medical School, 60 Fenwood Road, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women's Hospital, 60 Fenwood road, Boston, Massachusetts, USA"},{"name":"Department of Genetics, Harvard Medical School, 60 Fenwood Road, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5189-1227","authenticated-orcid":false,"given":"Olivier","family":"Tassy","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Universit\u00e9 de Strasbourg, Illkirch Graffenstaden, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Gobert","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Universit\u00e9 de Strasbourg, Illkirch Graffenstaden, France"},{"name":"Anagenesis Biotechnologies, Parc d'innovation - BioParc 3, 850 Boulevard S\u00e9bastien Brandt, 67400 Illkirch Graffenstaden, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Gapon","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women's Hospital, 60 Fenwood road, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Garnier","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Universit\u00e9 de Strasbourg, Illkirch Graffenstaden, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women's Hospital, 60 Fenwood road, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Hick","sequence":"additional","affiliation":[{"name":"Anagenesis Biotechnologies, Parc d'innovation - BioParc 3, 850 Boulevard S\u00e9bastien Brandt, 67400 Illkirch Graffenstaden, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arielle","family":"Hall","sequence":"additional","affiliation":[{"name":"Division of Genetics and Genomics, Boston Children's Hospital, 3 Blackfan Circle, CLS 15021, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Gussoni","sequence":"additional","affiliation":[{"name":"Division of Genetics and Genomics, Boston Children's Hospital, 3 Blackfan Circle, CLS 15021, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Pourqui\u00e9","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Universit\u00e9 de Strasbourg, Illkirch Graffenstaden, France"},{"name":"Department of Pathology, Brigham and Women's Hospital, 60 Fenwood road, Boston, Massachusetts, USA"},{"name":"Department of Genetics, Harvard Medical School, 60 Fenwood Road, Boston, Massachusetts, USA"},{"name":"Harvard Stem Cell Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042623460424400_DEV187344C1","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1016\/j.stem.2016.08.006","article-title":"CD82 is a marker for prospective isolation of human muscle satellite cells and is linked to muscular dystrophies","volume":"19","author":"Alexander","year":"2016","journal-title":"Cell Stem Cell"},{"key":"2021042623460424400_DEV187344C2","doi-asserted-by":"publisher","first-page":"e51576","DOI":"10.7554\/elife.51576","article-title":"Functionally heterogeneous human satellite cells identified by single cell RNA sequencing","volume":"9","author":"Barruet","year":"2020","journal-title":"eLife"},{"key":"2021042623460424400_DEV187344C3","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1038\/nbt.4314","article-title":"Dimensionality reduction for visualizing single-cell data using UMAP","volume":"37","author":"Becht","year":"2018","journal-title":"Nat. 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