{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:04:23Z","timestamp":1772168663428,"version":"3.50.1"},"reference-count":48,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 31571077"],"award-info":[{"award-number":["NSFC: 31571077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 81622012"],"award-info":[{"award-number":["NSFC: 81622012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014945","name":"Guangzhou Science and Technology Innovation Center","doi-asserted-by":"crossref","award":["201707020009"],"award-info":[{"award-number":["201707020009"]}],"id":[{"id":"10.13039\/501100014945","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002402","name":"Sun Yat-sen University","doi-asserted-by":"publisher","award":["8300-18821104"],"award-info":[{"award-number":["8300-18821104"]}],"id":[{"id":"10.13039\/501100002402","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State Key Laboratory of Ophthalmology at Zhongshan Ophthalmic Center","award":["303060202400339"],"award-info":[{"award-number":["303060202400339"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>In terrestrial animals, the lacrimal drainage apparatus evolved to serve as conduits for tear flow; however, little is known about the ontogenesis of this system. Here, we defined the anatomy of the fully formed tear duct (TD) in mice, characterized crucial morphogenetic events for the development of tear duct components, and identified the site for primordial tear duct (PTD) initiation. We report that the PTD originates from the orbital lacrimal lamina (LL), a junction formed by the epithelia of the maxillary (mxp) and lateral nasal processes (lnp). We demonstrate that Prickle 1, a key component of planar cell polarity (PCP) signaling, is expressed in progenitors of the PTD and throughout tear duct morphogenesis. Disruption of Prickle 1 stalls tear duct elongation; in particular, the loss of basement membrane (BM) deposition and aberrant cytoplasmic accumulation of laminin are salient. Altered cell adhesion, cytoskeletal and vesicular transport systems, and cell axis orientation in Prickle 1 mutants support the role of Prickle 1 in PCP. Taken together, we highlight a crucial role of Prickle 1-mediated polarized BM secretion and deposition in PTD elongation.<\/jats:p>","DOI":"10.1242\/dev.191726","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T19:13:15Z","timestamp":1604430795000},"source":"Crossref","is-referenced-by-count":2,"title":["Ontogenesis of the tear drainage system requires Prickle 1-driven polarized basement membrane (BM) deposition"],"prefix":"10.1242","author":[{"given":"Dianlei","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiali","family":"Ru","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuxiang","family":"Mao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Ouyang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Ju","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaili","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yizhi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1513-0073","authenticated-orcid":false,"given":"Chunqiao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042700491019400_DEV191726C1","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1097\/ICU.0000000000000092","article-title":"Hereditary disorders affecting the lacrimal system","volume":"25","author":"Allen","year":"2014","journal-title":"Curr. Opin Ophthalmol."},{"key":"2021042700491019400_DEV191726C2","doi-asserted-by":"publisher","first-page":"a027946","DOI":"10.1101\/cshperspect.a027946","article-title":"Signaling networks in epithelial tube formation","volume":"9","author":"Bernascone","year":"2017","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2021042700491019400_DEV191726C3","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1002\/ar.21366","article-title":"Timetable for upper eyelid development in staged human embryos and fetuses","volume":"294","author":"Byun","year":"2011","journal-title":"Anat. Rec."},{"key":"2021042700491019400_DEV191726C4","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1242\/dev.108944","article-title":"FGF signaling activates a Sox9-Sox10 pathway for the formation and branching morphogenesis of mouse ocular glands","volume":"141","author":"Chen","year":"2014","journal-title":"Development"},{"key":"2021042700491019400_DEV191726C5","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/j.1469-7580.2006.00606.x","article-title":"Morphogenesis of the human excretory lacrimal system","volume":"209","author":"de la Cuadra-Blanco","year":"2006","journal-title":"J. Anat."},{"key":"2021042700491019400_DEV191726C6","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1002\/jez.b.21205","article-title":"Tweaking the hinge and caps: testing a model of the organization of jaws","volume":"310B","author":"Depew","year":"2008","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"2021042700491019400_DEV191726C7","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.ydbio.2013.08.027","article-title":"Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation","volume":"383","author":"Dohn","year":"2013","journal-title":"Dev. Biol."},{"key":"2021042700491019400_DEV191726C8","doi-asserted-by":"publisher","first-page":"11187","DOI":"10.1073\/pnas.1403357111","article-title":"prickle modulates microtubule polarity and axonal transport to ameliorate seizures in flies","volume":"111","author":"Ehaideb","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042700491019400_DEV191726C9","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/j.devcel.2011.08.005","article-title":"A conserved Pbx-Wnt-p63-Irf6 regulatory module controls face morphogenesis by promoting epithelial apoptosis","volume":"21","author":"Ferretti","year":"2011","journal-title":"Dev. Cell"},{"key":"2021042700491019400_DEV191726C10","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1111\/cge.12321","article-title":"Identification of an IGSF3 mutation in a family with congenital nasolacrimal duct obstruction","volume":"86","author":"Foster","year":"2014","journal-title":"Clin. Genet."},{"key":"2021042700491019400_DEV191726C11","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1097\/IOP.0b013e3181b30358","article-title":"Identifying an appropriate animal model for the nasolacrimal drainage system","volume":"25","author":"Frame","year":"2009","journal-title":"Ophthalmic Plast Reconstr. Surg."},{"key":"2021042700491019400_DEV191726C12","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1016\/j.cub.2005.03.040","article-title":"Planar cell polarity genes regulate polarized extracellular matrix deposition during frog gastrulation","volume":"15","author":"Goto","year":"2005","journal-title":"Curr. Biol."},{"key":"2021042700491019400_DEV191726C13","doi-asserted-by":"publisher","first-page":"9277","DOI":"10.1073\/pnas.0402802101","article-title":"Frizzled6 controls hair patterning in mice","volume":"101","author":"Guo","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042700491019400_DEV191726C14","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1093\/icb\/icu067","article-title":"The Drosophila egg chamber-a new spin on how tissues elongate","volume":"54","author":"Horne-Badovinac","year":"2014","journal-title":"Integr Comp Biol"},{"key":"2021042700491019400_DEV191726C15","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1016\/j.ijporl.2005.12.015","article-title":"Characteristics of lacrimo-auriculo-dento-digital (LADD) syndrome: case report of a family and literature review","volume":"70","author":"Inan","year":"2006","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"2021042700491019400_DEV191726C16","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/j.jaapos.2006.06.008","article-title":"Ocular phenotype correlations in patients with TWIST versus FGFR3 genetic mutations","volume":"10","author":"Jadico","year":"2006","journal-title":"J. AAPOS"},{"key":"2021042700491019400_DEV191726C17","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/j.jaapos.2006.07.012","article-title":"Ocular abnormalities in Apert syndrome: genotype\/phenotype correlations with fibroblast growth factor receptor type 2 mutations","volume":"10","author":"Jadico","year":"2006","journal-title":"J. AAPOS"},{"key":"2021042700491019400_DEV191726C18","doi-asserted-by":"publisher","first-page":"23","DOI":"10.3109\/13816819009012945","article-title":"Familial Wolf-Hirschhorn syndrome associated with Rieger anomaly of the eye","volume":"11","author":"Kozma","year":"1990","journal-title":"Ophthalmic Paediatr. Genet."},{"key":"2021042700491019400_DEV191726C19","doi-asserted-by":"publisher","first-page":"3120","DOI":"10.1016\/j.cub.2017.09.011","article-title":"Disruption of core planar cell polarity signaling regulates renal tubule morphogenesis but is not cystogenic","volume":"27","author":"Kunimoto","year":"2017","journal-title":"Curr. Biol."},{"key":"2021042700491019400_DEV191726C20","doi-asserted-by":"publisher","first-page":"dev174888","DOI":"10.1242\/dev.174888","article-title":"The molecular anatomy of mammalian upper lip and primary palate fusion at single cell resolution","volume":"146","author":"Li","year":"2019","journal-title":"Development"},{"key":"2021042700491019400_DEV191726C21","doi-asserted-by":"publisher","first-page":"2234","DOI":"10.1093\/hmg\/ddt075","article-title":"Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis","volume":"22","author":"Liu","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"2021042700491019400_DEV191726C22","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1242\/bio.20148375","article-title":"Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a","volume":"3","author":"Liu","year":"2014","journal-title":"Biol. Open"},{"issue":"Suppl. 2","key":"2021042700491019400_DEV191726C23","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/S1010-5182(06)60003-6","article-title":"Apoptosis as a creative agent of embryonic development of bucca, mentum and nasolacrimal duct. An in vivo study in rats","volume":"34","author":"Lotz","year":"2006","journal-title":"J. Craniomaxillofac. Surg."},{"key":"2021042700491019400_DEV191726C24","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1111\/j.1469-7580.2009.01208.x","article-title":"The nasolacrimal duct of anuran amphibians: suggestions on its functional role in vomeronasal perception","volume":"216","author":"Nowack","year":"2010","journal-title":"J. Anat."},{"key":"2021042700491019400_DEV191726C25","first-page":"3137","article-title":"Animal model for the absorption of lipophilic substances from tear fluid by the epithelium of the nasolacrimal ducts","volume":"43","author":"Paulsen","year":"2002","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2021042700491019400_DEV191726C26","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1007\/s00347-001-0585-6","article-title":"[Nasolacrimal ducts and the dry eye]","volume":"99","author":"Paulsen","year":"2002","journal-title":"Ophthalmologe"},{"key":"2021042700491019400_DEV191726C27","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1007\/s00106-016-0164-4","article-title":"[Anatomy and physiology of the nasolacrimal ducts]","volume":"64","author":"Paulsen","year":"2016","journal-title":"HNO"},{"key":"2021042700491019400_DEV191726C28","first-page":"33","article-title":"The development of the eyelids. Part I. External features","volume":"130","author":"Pearson","year":"1980","journal-title":"J. Anat."},{"key":"2021042700491019400_DEV191726C29","doi-asserted-by":"publisher","first-page":"623186","DOI":"10.1155\/2011\/623186","article-title":"Development of the lacrimal apparatus in the rabbit (Oryctolagus cuniculus) and its potential role as an animal model for humans","volume":"2011","author":"Rehorek","year":"2011","journal-title":"Anat. Res. Int."},{"key":"2021042700491019400_DEV191726C30","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1002\/jmor.20393","article-title":"Development of the nasolacrimal apparatus in the Mongolian gerbil (Meriones unguiculatus), with notes on network topology and function","volume":"276","author":"Rehorek","year":"2015","journal-title":"J. Morphol."},{"key":"2021042700491019400_DEV191726C31","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1016\/j.cell.2014.10.010","article-title":"iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging","volume":"159","author":"Renier","year":"2014","journal-title":"Cell"},{"key":"2021042700491019400_DEV191726C32","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1038\/ng1757","article-title":"Mutations in different components of FGF signaling in LADD syndrome","volume":"38","author":"Rohmann","year":"2006","journal-title":"Nat. Genet."},{"key":"2021042700491019400_DEV191726C33","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1111\/j.1469-7580.2006.00682.x","article-title":"Ontogeny of the nasolacrimal duct in primates: functional and phylogenetic implications","volume":"210","author":"Rossie","year":"2007","journal-title":"J. Anat."},{"key":"2021042700491019400_DEV191726C100","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1167\/iovs.61.13.6","article-title":"Malformation of tear ducts underlies the epiphora and precocious eyelid opening in Prickle1 mutant mice: genetic implications for tear duct genesis","volume":"61","author":"Ru","year":"2020","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2021042700491019400_DEV191726C34","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1038\/ng.179","article-title":"Loss of Fat4 disrupts PCP signaling and oriented cell division and leads to cystic kidney disease","volume":"40","author":"Saburi","year":"2008","journal-title":"Nat. Genet."},{"key":"2021042700491019400_DEV191726C35","doi-asserted-by":"publisher","first-page":"3048740","DOI":"10.1155\/2019\/3048740","article-title":"Dry eye analysis: a citation network study","volume":"2019","author":"Sanchez-Tena","year":"2019","journal-title":"J. Ophthalmol."},{"key":"2021042700491019400_DEV191726C36","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/eye.1988.25","article-title":"A reappraisal of the development of the eyelids","volume":"2","author":"Sevel","year":"1988","journal-title":"Eye (Lond)"},{"key":"2021042700491019400_DEV191726C37","first-page":"563","article-title":"Facial and visceral arch development in the mouse embryo: a study by scanning electron microscopy","volume":"124","author":"Tamarin","year":"1977","journal-title":"J. Anat."},{"key":"2021042700491019400_DEV191726C38","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1097\/IOP.0000000000000702","article-title":"Embryologic and fetal development of the human eyelid","volume":"32","author":"Tawfik","year":"2016","journal-title":"Ophthalmic Plast. Reconstr. Surg."},{"key":"2021042700491019400_DEV191726C39","doi-asserted-by":"publisher","first-page":"96","DOI":"10.3390\/diseases6040096","article-title":"Congenital Nasolacrimal Duct Obstruction (CNLDO): A Review","volume":"6","author":"Vagge","year":"2018","journal-title":"Diseases"},{"key":"2021042700491019400_DEV191726C40","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1136\/bjo.84.10.1177","article-title":"Ocular features in Rubinstein-Taybi syndrome: investigation of 24 patients and review of the literature","volume":"84","author":"van Genderen","year":"2000","journal-title":"Br. J. Ophthalmol."},{"key":"2021042700491019400_DEV191726C41","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1242\/dev.010892","article-title":"Chongmague reveals an essential role for laminin-mediated boundary formation in chordate convergence and extension movements","volume":"135","author":"Veeman","year":"2008","journal-title":"Development"},{"key":"2021042700491019400_DEV191726C42","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1038\/sj.eye.6702049","article-title":"Endoscopic-assisted probing for congenital nasolacrimal duct obstruction","volume":"20","author":"Wallace","year":"2006","journal-title":"Eye"},{"key":"2021042700491019400_DEV191726C43","doi-asserted-by":"publisher","first-page":"5815","DOI":"10.1242\/dev.00123","article-title":"Neural tube closure requires Dishevelled-dependent convergent extension of the midline","volume":"129","author":"Wallingford","year":"2002","journal-title":"Development"},{"key":"2021042700491019400_DEV191726C44","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1016\/S1534-5807(02)00197-1","article-title":"Convergent extension: the molecular control of polarized cell movement during embryonic development","volume":"2","author":"Wallingford","year":"2002","journal-title":"Dev. Cell"},{"key":"2021042700491019400_DEV191726C45","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1523\/JNEUROSCI.4698-05.2005","article-title":"The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells","volume":"26","author":"Wang","year":"2006","journal-title":"J. Neurosci."},{"key":"2021042700491019400_DEV191726C46","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1002\/dvdy.24025","article-title":"Prickle1 stunts limb growth through alteration of cell polarity and gene expression","volume":"242","author":"Yang","year":"2013","journal-title":"Dev. Dyn."},{"key":"2021042700491019400_DEV191726C47","doi-asserted-by":"publisher","first-page":"4383","DOI":"10.1242\/dev.083352","article-title":"Frizzled 2 and frizzled 7 function redundantly in convergent extension and closure of the ventricular septum and palate: evidence for a network of interacting genes","volume":"139","author":"Yu","year":"2012","journal-title":"Development"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/dev.191726","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.191726\/1972071\/dev191726.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.191726\/2065244\/dev_191726v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.191726\/2065244\/dev_191726v1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T18:50:40Z","timestamp":1621363840000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/doi\/10.1242\/dev.191726\/267020\/Ontogenesis-of-the-tear-drainage-system-requires"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,1]]},"references-count":48,"URL":"https:\/\/doi.org\/10.1242\/dev.191726","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.07.029793","asserted-by":"object"}]},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1,1]]},"article-number":"dev.191726"}}