{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:34:20Z","timestamp":1792629260781,"version":"4.5.0"},"reference-count":62,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["Intramural Research Program of the National Institutes of Health"],"award-info":[{"award-number":["Intramural Research Program of the National Institutes of Health"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001869","name":"Academia Sinica","doi-asserted-by":"publisher","award":["Intramural Funds"],"award-info":[{"award-number":["Intramural Funds"]}],"id":[{"id":"10.13039\/501100001869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CAREER Award and Research Corporation for Scientific Advancement Cottrell College Award"],"award-info":[{"award-number":["CAREER Award and Research Corporation for Scientific Advancement Cottrell College Award"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>SEIPIN, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed C. elegans mutants deleted of the sole SEIPIN gene, seip-1. Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation; these experiments support a great potential of using C. elegans to model SEIPIN-associated human diseases.<\/jats:p>","DOI":"10.1242\/dev.192997","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T17:40:13Z","timestamp":1597945213000},"source":"Crossref","is-referenced-by-count":29,"title":["Loss of the seipin gene perturbs eggshell formation in\n                    <i>C. elegans<\/i>"],"prefix":"10.1242","author":[{"given":"Xiaofei","family":"Bai","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leng-Jie","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Nebenfuhr","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA"},{"name":"Current address: Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Wysolmerski","sequence":"additional","affiliation":[{"name":"Department of Biology and Program in Molecular Biology, Pomona College, Claremont, CA 91711, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jui-Ching","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara K.","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Biology and Program in Molecular Biology, Pomona College, Claremont, CA 91711, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8599-2031","authenticated-orcid":false,"given":"Andy","family":"Golden","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4073-299X","authenticated-orcid":false,"given":"Chao-Wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042700401641800_DEV192997C1","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.mod.2009.02.001","article-title":"Two cytochrome P450s in Caenorhabditis elegans are essential for the organization of eggshell, correct execution of meiosis and the polarization of embryo","volume":"126","author":"Benenati","year":"2009","journal-title":"Mech. 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