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Anterior and posterior PAs appear to be generated by different mechanisms, but it is unclear how the anterior and posterior PAs combine. Here, we addressed this issue with precise live imaging of PP development and cell tracing of pharyngeal endoderm in zebrafish embryos. We found that two endodermal bulges are initially generated in the future second PP (PP2) region, which separates anterior and posterior PAs. Subsequently, epithelial remodeling causes contact between these two bulges, resulting in the formation of mature PP2 with a bilayered morphology. The rostral and caudal bulges develop into the operculum and gill, respectively. Development of the caudal PP2 and more posterior PPs is affected by impaired retinoic acid signaling or pax1a\/b dysfunction, suggesting that the rostral front of posterior PA development corresponds to the caudal PP2. Our study clarifies an aspect of PA development that is essential for generation of a seamless array of PAs in zebrafish.<\/jats:p>","DOI":"10.1242\/dev.194738","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T17:00:54Z","timestamp":1604682054000},"source":"Crossref","is-referenced-by-count":10,"title":["The second pharyngeal pouch is generated by dynamic remodeling of endodermal epithelium in zebrafish"],"prefix":"10.1242","volume":"147","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6180-661X","authenticated-orcid":false,"given":"Kazunori","family":"Okada","sequence":"first","affiliation":[{"name":"1 Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaijicho, Okazaki 444-8787, Japan"},{"name":"National Institute for Basic Biology, National Institutes of Natural Sciences 2 , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4125-6056","authenticated-orcid":false,"given":"Shinji","family":"Takada","sequence":"additional","affiliation":[{"name":"1 Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaijicho, Okazaki 444-8787, Japan"},{"name":"National Institute for Basic Biology, National Institutes of Natural Sciences 2 , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 , Japan"},{"name":"SOKENDAI (The Graduate University for Advanced Studies) 3 Department for Basic Biology , , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,12,23]]},"reference":[{"key":"2022081206472461400_DEV194738C1","doi-asserted-by":"publisher","first-page":"dev167965","DOI":"10.1242\/dev.167965","article-title":"Region-specific endodermal signals direct neural crest cells to form the three middle ear ossicles","volume":"146","author":"Ankamreddy","year":"2019","journal-title":"Development"},{"key":"2022081206472461400_DEV194738C2","doi-asserted-by":"publisher","first-page":"20152210","DOI":"10.1098\/rspb.2015.2210","article-title":"The hyoid arch and braincase anatomy of Acanthodes support chondrichthyan affinity of \u2018acanthodians","volume":"282","author":"Brazeau","year":"2015","journal-title":"Proc. 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