{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:15:36Z","timestamp":1790475336502,"version":"4.1.0"},"reference-count":52,"publisher":"The Company of Biologists","issue":"16","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"crossref","award":["RF1MH121539"],"award-info":[{"award-number":["RF1MH121539"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["SAF-2017-82880-R"],"award-info":[{"award-number":["SAF-2017-82880-R"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["RyC-2015-19058"],"award-info":[{"award-number":["RyC-2015-19058"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["717115-3"],"award-info":[{"award-number":["717115-3"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Conselleria de Educaci\u00f3n de Valencia","award":["GJIDI\/2018\/A\/221"],"award-info":[{"award-number":["GJIDI\/2018\/A\/221"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Neocortical progenitor cells generate subtypes of excitatory projection neurons in sequential order followed by the generation of astrocytes. The transcription factor zinc finger and BTB domain-containing protein 20 (ZBTB20) has been implicated in regulation of cell specification during neocortical development. Here, we show that ZBTB20 instructs the generation of a subset of callosal projections neurons in cortical layers II\/III in mouse. Conditional deletion of Zbtb20 in cortical progenitors, and to a lesser degree in differentiating neurons, leads to an increase in the number of layer IV neurons at the expense of layer II\/III neurons. Astrogliogenesis is also affected in the mutants with an increase in the number of a specific subset of astrocytes expressing GFAP. Astrogliogenesis is more severely disrupted by a ZBTB20 protein containing dominant mutations linked to Primrose syndrome, suggesting that ZBTB20 acts in concert with other ZBTB proteins that were also affected by the dominant-negative protein to instruct astrogliogenesis. Overall, our data suggest that ZBTB20 acts both in progenitors and in postmitotic cells to regulate cell fate specification in the mammalian neocortex.<\/jats:p>","DOI":"10.1242\/dev.196642","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T14:49:16Z","timestamp":1628174956000},"source":"Crossref","is-referenced-by-count":20,"title":["ZBTB20 is crucial for the specification of a subset of callosal projection neurons and astrocytes in the mammalian neocortex"],"prefix":"10.1242","volume":"148","author":[{"given":"J\u00e9ssica Alves","family":"Medeiros de Ara\u00fajo","sequence":"first","affiliation":[{"name":"The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA"},{"name":"Brain Institute, Federal University of Rio Grande do Norte, Natal, RN 59056-450, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraia","family":"Bar\u00e3o","sequence":"additional","affiliation":[{"name":"The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Mateos-White","sequence":"additional","affiliation":[{"name":"BIOTECMED Institute, Universidad de Valencia, Burjassot, Valencia 46100, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Espinosa","sequence":"additional","affiliation":[{"name":"AntalGenics, Quorum Building III, Scientific Park - UMH. Avda. de la Universidad, s\/n. 03202 Elche (Alicante), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Romualdo","family":"Costa","sequence":"additional","affiliation":[{"name":"Brain Institute, Federal University of Rio Grande do Norte, Natal, RN 59056-450, Brazil"},{"name":"Unit\u00e9 INSERM 1167, RID-AGE-Risk Factors and Molecular Determinants of Aging-Related Diseases, Institut Pasteur de Lille, University of Lille, U1167-Excellence Laboratory LabEx DISTALZ, Lille Cedex 59019, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7827-5480","authenticated-orcid":false,"given":"Cristina","family":"Gil-Sanz","sequence":"additional","affiliation":[{"name":"BIOTECMED Institute, Universidad de Valencia, Burjassot, Valencia 46100, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,8,19]]},"reference":[{"key":"2021081908552620300_DEV196642C1","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1002\/ajmg.a.38684","article-title":"Novel de novo ZBTB20 mutations in three cases with Primrose syndrome and constant corpus callosum anomalies","volume":"176","author":"Alby","year":"2018","journal-title":"Am. J. Med. Genet. Part A"},{"key":"2021081908552620300_DEV196642C2","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.neuron.2007.12.012","article-title":"Satb2 regulates callosal projection neuron identity in the developing cerebral cortex","volume":"57","author":"Alcamo","year":"2008","journal-title":"Neuron"},{"key":"2021081908552620300_DEV196642C3","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1523\/JNEUROSCI.2986-07.2008","article-title":"Ctip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum","volume":"28","author":"Arlotta","year":"2008","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C4","doi-asserted-by":"publisher","first-page":"14108","DOI":"10.1073\/pnas.0804650106","article-title":"Changing numbers of neuronal and non-neuronal cells underlie postnatal brain growth in the rat","volume":"106","author":"Bandeira","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021081908552620300_DEV196642C5","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/nn.3371","article-title":"Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation","volume":"16","author":"Bardehle","year":"2013","journal-title":"Nat. Neurosci."},{"key":"2021081908552620300_DEV196642C6","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1038\/s41593-020-0602-1","article-title":"Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map","volume":"23","author":"Bayraktar","year":"2020","journal-title":"Nat. Neurosci."},{"key":"2021081908552620300_DEV196642C7","doi-asserted-by":"publisher","first-page":"13854","DOI":"10.1523\/JNEUROSCI.4494-07.2007","article-title":"\u03921 integrins in radial glia but not in migrating neurons are essential for the formation of cell layers in the cerebral cortex","volume":"27","author":"Belvindrah","year":"2007","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C8","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1002\/ajmg.a.38124","article-title":"Alterations in metabolic patterns have a key role in diagnosis and progression of primrose syndrome","volume":"173","author":"Casertano","year":"2017","journal-title":"Am. J. Med. Genet. Part A"},{"key":"2021081908552620300_DEV196642C9","doi-asserted-by":"publisher","first-page":"8863","DOI":"10.1523\/JNEUROSCI.21-22-08863.2001","article-title":"Specific neurotrophic factors support the survival of cortical projection neurons at distinct stages of development","volume":"21","author":"Catapano","year":"2001","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C10","doi-asserted-by":"publisher","first-page":"E1896","DOI":"10.1073\/pnas.1800165115","article-title":"Normal aging induces A1-like astrocyte reactivity","volume":"115","author":"Clarke","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021081908552620300_DEV196642C11","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1002\/ajmg.a.61024","article-title":"Refining the Primrose syndrome phenotype: a study of five patients with ZBTB20 de novo variants and a review of the literature","volume":"179","author":"Cleaver","year":"2019","journal-title":"Am. J. Med. Genet. Part A"},{"key":"2021081908552620300_DEV196642C12","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1038\/ng.3035","article-title":"Mutations in ZBTB20 cause Primrose syndrome","volume":"46","author":"Cordeddu","year":"2014","journal-title":"Nat. Genet."},{"key":"2021081908552620300_DEV196642C13","doi-asserted-by":"publisher","first-page":"R56","DOI":"10.1186\/gb-2014-15-4-r56","article-title":"Hypermethylation in the ZBTB20 gene is associated with major depressive disorder","volume":"15","author":"Davies","year":"2014","journal-title":"Genome Biol."},{"key":"2021081908552620300_DEV196642C14","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/j.mcn.2004.07.008","article-title":"Nuclear expression of S100B in oligodendrocyte progenitor cells correlates with differentiation toward the oligodendroglial lineage and modulates oligodendrocytes maturation","volume":"27","author":"Deloulme","year":"2004","journal-title":"Mol. Cell. Neurosci."},{"key":"2021081908552620300_DEV196642C15","doi-asserted-by":"publisher","first-page":"2632","DOI":"10.1093\/cercor\/bhs252","article-title":"POU-III transcription factors (Brn1, Brn2, and Oct6) influence neurogenesis, molecular identity, and migratory destination of upper-layer cells of the cerebral cortex","volume":"23","author":"Dominguez","year":"2013","journal-title":"Cereb. Cortex"},{"key":"2021081908552620300_DEV196642C16","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/nature10959","article-title":"Local generation of glia is a major astrocyte source in postnatal cortex","volume":"484","author":"Ge","year":"2012","journal-title":"Nature"},{"key":"2021081908552620300_DEV196642C17","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1002\/dvg.20256","article-title":"Genetic targeting of principal neurons in neocortex and hippocampus of NEX-Cre mice","volume":"44","author":"Goebbels","year":"2006","journal-title":"Wiley Interscipl. Rev. Dev. Biol."},{"key":"2021081908552620300_DEV196642C18","doi-asserted-by":"publisher","first-page":"6309","DOI":"10.1523\/JNEUROSCI.22-15-06309.2002","article-title":"Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage","volume":"22","author":"Gorski","year":"2002","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C19","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1038\/nrn3586","article-title":"Molecular logic of neocortical projection neuron specification, development and diversity","volume":"14","author":"Greig","year":"2013","journal-title":"Nat. Rev. Neurosci."},{"key":"2021081908552620300_DEV196642C20","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1016\/j.neuron.2015.07.030","article-title":"Wide dispersion and diversity of clonally related inhibitory interneurons","volume":"87","author":"Harwell","year":"2015","journal-title":"Neuron"},{"key":"2021081908552620300_DEV196642C21","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1523\/JNEUROSCI.02-06-00735.1982","article-title":"Ontogenetic changes in the projections of neocortical neurons","volume":"2","author":"Ivy","year":"1982","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C22","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1093\/cercor\/bhr182","article-title":"ROR\u03b2 induces barrel-like neuronal clusters in the developing neocortex","volume":"22","author":"Jabaudon","year":"2012","journal-title":"Cereb. Cortex"},{"key":"2021081908552620300_DEV196642C23","doi-asserted-by":"publisher","first-page":"e0203760","DOI":"10.1371\/journal.pone.0203760","article-title":"Neurodevelopmental disorder-associated ZBTB20 gene variants affect dendritic and synaptic structure","volume":"13","author":"Jones","year":"2018","journal-title":"PLoS ONE"},{"key":"2021081908552620300_DEV196642C24","author":"Koul","year":"2014"},{"key":"2021081908552620300_DEV196642C25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3791\/56063","article-title":"Live imaging of primary cerebral cortex cells using a 2D culture system","volume":"2017","author":"Landeira","year":"2017","journal-title":"J. Vis. Exp."},{"key":"2021081908552620300_DEV196642C26","doi-asserted-by":"publisher","first-page":"3406","DOI":"10.1093\/cercor\/bhu156","article-title":"Satb2 regulates the differentiation of both callosal and subcerebral projection neurons in the developing cerebral cortex","volume":"25","author":"Leone","year":"2015","journal-title":"Cereb. Cortex"},{"key":"2021081908552620300_DEV196642C28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1523\/ENEURO.0234-17.2017","article-title":"Caveolin1 identifies a specific subpopulation of cerebral cortex callosal projection neurons (CPN) including dual projecting cortical callosal\/frontal projection neurons (CPN\/FPN)","volume":"5","author":"MacDonald","year":"2018","journal-title":"eNeuro"},{"key":"2021081908552620300_DEV196642C29","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"Madisen","year":"2010","journal-title":"Nat. Neurosci."},{"key":"2021081908552620300_DEV196642C31","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.1002\/ajmg.a.37645","article-title":"Novel de novo mutations in ZBTB20 in Primrose syndrome with congenital hypothyroidism","volume":"170","author":"Mattioli","year":"2016","journal-title":"Am. J. Med. Genet. Part A"},{"key":"2021081908552620300_DEV196642C32","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.neuron.2007.04.019","article-title":"Timing is everything: making neurons versus glia in the developing cortex","volume":"54","author":"Miller","year":"2007","journal-title":"Neuron"},{"key":"2021081908552620300_DEV196642C33","doi-asserted-by":"publisher","first-page":"7598","DOI":"10.1074\/jbc.M110023200","article-title":"Characterization of two novel nuclear BTB\/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia","volume":"277","author":"Mitchelmore","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2021081908552620300_DEV196642C34","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1038\/nrn2151","article-title":"Neuronal subtype specification in the cerebral cortex","volume":"8","author":"Molyneaux","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"2021081908552620300_DEV196642C35","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.neuron.2014.12.024","article-title":"DeCoN: Genome-wide analysis of in vivo transcriptional dynamics during pyramidal neuron fate selection in neocortex","volume":"85","author":"Molyneaux","year":"2015","journal-title":"Neuron"},{"key":"2021081908552620300_DEV196642C36","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.cancergen.2016.05.050","article-title":"Microdeletion of ZBTB20 results in a phenotype overlapping with that of the microdeletion 3q13.31 syndrome","volume":"209","author":"Mulatinho","year":"2016","journal-title":"Cancer Genet."},{"key":"2021081908552620300_DEV196642C37","doi-asserted-by":"publisher","first-page":"11102","DOI":"10.1038\/ncomms11102","article-title":"Zbtb20 promotes astrocytogenesis during neocortical development","volume":"7","author":"Nagao","year":"2016","journal-title":"Nat. Commun."},{"key":"2021081908552620300_DEV196642C38","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/0959-4388(94)90054-X","article-title":"Development, critical period plasticity, and adult reorganizations of mammalian somatosensory systems","volume":"4","author":"O'Leary","year":"1994","journal-title":"Curr. Opin. Neurobiol."},{"key":"2021081908552620300_DEV196642C39","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1002\/glia.20445","article-title":"S100B expression defines a state in which GFAP- expressing cells lose their neural stem cell potential and acquire a more mature developmental stage","volume":"55","author":"Raponi","year":"2007","journal-title":"Glia"},{"key":"2021081908552620300_DEV196642C40","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1136\/jmedgenet-2014-102535","article-title":"Neurodevelopmental disorders associated with dosage imbalance of ZBTB20 correlate with the morbidity spectrum of ZBTB20 candidate target genes","volume":"51","author":"Rasmussen","year":"2014","journal-title":"J. Med. Genet."},{"key":"2021081908552620300_DEV196642C41","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/75973","article-title":"High-efficiency deleter mice show that FLPe is an alternative to Cre- loxP","volume":"25","author":"Rodr\u00edguez","year":"2000","journal-title":"Nat. Genet."},{"key":"2021081908552620300_DEV196642C42","doi-asserted-by":"publisher","first-page":"2144","DOI":"10.1002\/hipo.22035","article-title":"Regulation of archicortical arealization by the transcription factor Zbtb20","volume":"22","author":"Rosenthal","year":"2012","journal-title":"Hippocampus"},{"key":"2021081908552620300_DEV196642C43","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1038\/nature09611","article-title":"Developmental genetics of vertebrate glial-cell specification","volume":"468","author":"Rowitch","year":"2010","journal-title":"Nature"},{"key":"2021081908552620300_DEV196642C44","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1006\/dbio.2001.0439","article-title":"Efficient gene transfer into the embryonic mouse brain using in vivo electroporation","volume":"240","author":"Saito","year":"2001","journal-title":"Dev. Biol."},{"key":"2021081908552620300_DEV196642C45","doi-asserted-by":"publisher","first-page":"3358","DOI":"10.4161\/cc.21277","article-title":"The BTB-ZF transcription factors","volume":"11","author":"Siggs","year":"2012","journal-title":"Cell Cycle"},{"key":"2021081908552620300_DEV196642C46","doi-asserted-by":"publisher","first-page":"4493","DOI":"10.1523\/JNEUROSCI.3199-16.2017","article-title":"SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions","volume":"37","author":"Sun","year":"2017","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C47","doi-asserted-by":"publisher","first-page":"5837","DOI":"10.1523\/JNEUROSCI.5048-06.2007","article-title":"Antigen-retrieval procedure for bromodeoxyuridine immunolabeling with concurrent labeling of nuclear DNA and antigens damaged by HCl pretreatment","volume":"27","author":"Tang","year":"2007","journal-title":"J. Neurosci."},{"key":"2021081908552620300_DEV196642C48","doi-asserted-by":"publisher","first-page":"eaav2522","DOI":"10.1126\/science.aav2522","article-title":"Temporal patterning of apical progenitors and their daughter neurons in the developing neocortex","volume":"364","author":"Telley","year":"2019","journal-title":"Science (80-)"},{"key":"2021081908552620300_DEV196642C49","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s13041-016-0242-2","article-title":"Zbtb20 modulates the sequential generation of neuronal layers in developing cortex","volume":"9","author":"Tonchev","year":"2016","journal-title":"Mol. Brain"},{"key":"2021081908552620300_DEV196642C50","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","article-title":"RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues","volume":"14","author":"Wang","year":"2012","journal-title":"J. Mol. Diagnostics"},{"key":"2021081908552620300_DEV196642C51","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1002\/cne.901780202","article-title":"Developmental studies of thalamocortical and commissural connections in the rat somatic sensory cortex","volume":"178","author":"Wise","year":"1978","journal-title":"J. Comp. Neurol."},{"key":"2021081908552620300_DEV196642C52","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/0006-8993(70)90079-X","article-title":"The structural organization of layer IV in the somatosensory region (S I) of mouse cerebral cortex. The description of a cortical field composed of discrete cytoarchitectonic units","volume":"17","author":"Woolsey","year":"1970","journal-title":"Brain Res."},{"key":"2021081908552620300_DEV196642C53","doi-asserted-by":"publisher","first-page":"17172","DOI":"10.1073\/pnas.0508560102","article-title":"Pyramidal neurons of upper cortical layers generated by NEX-positive progenitor cells in the subventricular zone","volume":"102","author":"Wu","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021081908552620300_DEV196642C54","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1038\/nature07722","article-title":"Specific synapses develop preferentially among sister excitatory neurons in the neocortex","volume":"458","author":"Yu","year":"2009","journal-title":"Nature"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.196642\/2092819\/dev196642.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.196642\/2101267\/dev196642.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.196642\/2101267\/dev196642.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T08:55:46Z","timestamp":1629363346000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/148\/16\/dev196642\/271872\/ZBTB20-is-crucial-for-the-specification-of-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,15]]},"references-count":52,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2021,8,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.196642","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,15]]},"article-number":"dev196642"}}