{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:04:34Z","timestamp":1772168674585,"version":"3.50.1"},"reference-count":38,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEECIND\/00333\/2017"],"award-info":[{"award-number":["CEECIND\/00333\/2017"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEECIND\/01967\/2017"],"award-info":[{"award-number":["CEECIND\/01967\/2017"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["DL57\/2016\/CP1355\/CT0007"],"award-info":[{"award-number":["DL57\/2016\/CP1355\/CT0007"]}]},{"DOI":"10.13039\/501100006111","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"crossref","award":["PTDC\/BIA-CEL\/30507\/2017"],"award-info":[{"award-number":["PTDC\/BIA-CEL\/30507\/2017"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The microtubule motor cytoplasmic dynein 1 (dynein) and its essential activator dynactin have conserved roles in spindle assembly and positioning during female meiosis and mitosis, but their contribution to male meiosis remains poorly understood. Here, we characterize the G33S mutation in the C. elegans dynactin subunit DNC-1, which corresponds to G59S in human p150Glued that causes motor neuron disease. In spermatocytes, dnc-1(G33S) delays spindle assembly and penetrantly inhibits anaphase spindle elongation in meiosis I, which prevents the segregation of homologous chromosomes. By contrast, chromosomes segregate without errors in the early dnc-1(G33S) embryo. Deletion of the DNC-1 N-terminus shows that defective meiosis in dnc-1(G33S) spermatocytes is not due to the inability of DNC-1 to interact with microtubules. Instead, our results suggest that the DNC-1(G33S) protein, which is aggregation prone in vitro, is less stable in spermatocytes than the early embryo, resulting in different phenotypic severity in the two dividing tissues. Thus, the dnc-1(G33S) mutant reveals that dynein-dynactin drive meiotic chromosome segregation in spermatocytes and illustrates that the extent to which protein misfolding leads to loss of function can vary significantly between cell types.<\/jats:p>","DOI":"10.1242\/dev.197780","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T11:36:21Z","timestamp":1610969781000},"source":"Crossref","is-referenced-by-count":3,"title":["Dynein-dynactin segregate meiotic chromosomes in\n                    <i>C. elegans<\/i>\n                    spermatocytes"],"prefix":"10.1242","volume":"148","author":[{"given":"Daniel J.","family":"Barbosa","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade - i3S, Universidade do Porto, 4200-135 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade - i3S, Universidade do Porto, 4200-135 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Duro","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade - i3S, Universidade do Porto, 4200-135 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana X.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade - i3S, Universidade do Porto, 4200-135 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0360-2977","authenticated-orcid":false,"given":"Reto","family":"Gassmann","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade - i3S, Universidade do Porto, 4200-135 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2021,2,10]]},"reference":[{"key":"2021042623024403900_DEV197780C1","doi-asserted-by":"publisher","first-page":"5083","DOI":"10.1021\/bi100235z","article-title":"Disease-associated mutations in the p150(Glued) subunit destabilize the CAP-gly domain","volume":"49","author":"Ahmed","year":"2010","journal-title":"Biochemistry"},{"key":"2021042623024403900_DEV197780C2","doi-asserted-by":"publisher","first-page":"2709","DOI":"10.1091\/mbc.e08-12-1165","article-title":"asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis","volume":"20","author":"Anderson","year":"2009","journal-title":"Mol. 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Struct. Biol."},{"key":"2021042623024403900_DEV197780C6","doi-asserted-by":"publisher","first-page":"1946","DOI":"10.1093\/hmg\/ddn092","article-title":"Lysosomal proliferation and distal degeneration in motor neurons expressing the G59S mutation in the p150Glued subunit of dynactin","volume":"17","author":"Chevalier-Larsen","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"2021042623024403900_DEV197780C7","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1016\/j.devcel.2004.10.015","article-title":"Centriole assembly requires both centriolar and pericentriolar material proteins","volume":"7","author":"Dammermann","year":"2004","journal-title":"Dev. Cell"},{"key":"2021042623024403900_DEV197780C8","doi-asserted-by":"publisher","first-page":"e202005179","DOI":"10.1083\/jcb.202005179","article-title":"Evidence for anaphase pulling forces during C. elegans meiosis","volume":"219","author":"Danlasky","year":"2020","journal-title":"J. Cell Biol."},{"key":"2021042623024403900_DEV197780C9","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1038\/ncb2093","article-title":"A kinetochore-independent mechanism drives anaphase chromosome separation during acentrosomal meiosis","volume":"12","author":"Dumont","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"2021042623024403900_DEV197780C10","doi-asserted-by":"publisher","first-page":"e50988","DOI":"10.7554\/eLife.50988","article-title":"Male meiotic spindle features that efficiently segregate paired and lagging chromosomes","volume":"9","author":"Fabig","year":"2020","journal-title":"eLife"},{"key":"2021042623024403900_DEV197780C11","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/ng.293","article-title":"DCTN1 mutations in Perry syndrome","volume":"41","author":"Farrer","year":"2009","journal-title":"Nat. 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