{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:41:44Z","timestamp":1789807304973,"version":"4.0.1"},"reference-count":54,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["5R01DK083289"],"award-info":[{"award-number":["5R01DK083289"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Ret signaling promotes branching morphogenesis during kidney development, but the underlying cellular mechanisms remain unclear. While Ret-expressing progenitor cells proliferate at the ureteric bud tips, some of these cells exit the tips to generate the elongating collecting ducts, and in the process turn off Ret. Genetic ablation of Ret in tip cells promotes their exit, suggesting that Ret is required for cell rearrangements that maintain the tip compartments. Here, we examine the behaviors of ureteric bud cells that are genetically forced to maintain Ret expression. These cells move to the nascent tips, and remain there during many cycles of branching; this tip-seeking behavior may require positional signals from the mesenchyme, as it occurs in whole kidneys but not in epithelial ureteric bud organoids. In organoids, cells forced to express Ret display a striking self-organizing behavior, attracting each other to form dense clusters within the epithelium, which then evaginate to form new buds. The ability of forced Ret expression to promote these events suggests that similar Ret-dependent cell behaviors play an important role in normal branching morphogenesis.<\/jats:p>","DOI":"10.1242\/dev.199386","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T15:15:07Z","timestamp":1619709307000},"source":"Crossref","is-referenced-by-count":16,"title":["Ret signaling in ureteric bud epithelial cells controls cell movements, cell clustering and bud formation"],"prefix":"10.1242","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2535-3921","authenticated-orcid":false,"given":"Adam","family":"Packard","sequence":"first","affiliation":[{"name":"Department of Genetics and Development, Columbia University, New York, NY 10032, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Genetics and Development, Columbia University, New York, NY 10032, USA"},{"name":"Department of Systems Biology, MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3050-9719","authenticated-orcid":false,"given":"Frank","family":"Costantini","sequence":"additional","affiliation":[{"name":"Department of Genetics and Development, Columbia University, New York, NY 10032, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"2021051821365580500_DEV199386C1","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1038\/sj.onc.1200896","article-title":"Identification of Shc docking site on Ret tyrosine kinase","volume":"14","author":"Arighi","year":"1997","journal-title":"Oncogene"},{"key":"2021051821365580500_DEV199386C2","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.ydbio.2006.08.051","article-title":"Branching morphogenesis of the ureteric epithelium during kidney development is coordinated by the opposing functions of GDNF and Sprouty1","volume":"299","author":"Basson","year":"2006","journal-title":"Dev. 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