{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:24:35Z","timestamp":1790760275906,"version":"4.1.0"},"reference-count":32,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T00:00:00Z","timestamp":1623715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["GNT1107831"],"award-info":[{"award-number":["GNT1107831"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["GNT1046092"],"award-info":[{"award-number":["GNT1046092"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ets homologous factor (EHF) is a member of the epithelial-specific Ets (ESE) family of transcription factors. To investigate its role in development and epithelial homeostasis, we generated a series of novel mouse strains in which the Ets DNA-binding domain of Ehf was deleted in all tissues (Ehf\u2212\/\u2212) or specifically in the gut epithelium. Ehf\u2212\/\u2212 mice were born at the expected Mendelian ratio, but showed reduced body weight gain, and developed a series of pathologies requiring most Ehf\u2212\/\u2212 mice to reach an ethical endpoint before reaching 1 year of age. These included papillomas in the facial skin, abscesses in the preputial glands (males) or vulvae (females), and corneal ulcers. Ehf\u2212\/\u2212mice also displayed increased susceptibility to experimentally induced colitis, which was confirmed in intestinal-specific Ehf knockout mice. Gut-specific Ehf deletion also impaired goblet cell differentiation, induced extensive transcriptional reprogramming in the colonic epithelium and enhanced Apc-initiated adenoma development. The Ets DNA-binding domain of EHF is therefore essential for postnatal homeostasis of the epidermis and colonic epithelium, and its loss promotes colonic tumour development.<\/jats:p>","DOI":"10.1242\/dev.199542","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T10:21:17Z","timestamp":1625134877000},"source":"Crossref","is-referenced-by-count":22,"title":["EHF is essential for epidermal and colonic epithelial homeostasis, and suppresses\n                    <i>Apc<\/i>\n                    -initiated colonic tumorigenesis"],"prefix":"10.1242","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8039-3582","authenticated-orcid":false,"given":"Camilla M.","family":"Reehorst","sequence":"first","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 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3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Koentgen","sequence":"additional","affiliation":[{"name":"Ozgene, Perth, Western Australia, 6983Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Williams","sequence":"additional","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charbel","family":"Darido","sequence":"additional","affiliation":[{"name":"Peter MacCallum Cancer Centre, Melbourne, 3000Australia"},{"name":"Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, 3010Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Tan","sequence":"additional","affiliation":[{"name":"Peter MacCallum Cancer Centre, Melbourne, 3000Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly","family":"Anderton","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute, Melbourne, 3052Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Chopin","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute, Melbourne, 3052Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Victoria, 3010Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kael","family":"Schoffer","sequence":"additional","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz F.","family":"Eissmann","sequence":"additional","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Buchert","sequence":"additional","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri","family":"Mouradov","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute, Melbourne, 3052Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver M.","family":"Sieber","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute, Melbourne, 3052Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Victoria, 3010Australia"},{"name":"Department of Surgery, The University of Melbourne, Parkville, Victoria, 3010Australia"},{"name":"Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3800Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Ernst","sequence":"additional","affiliation":[{"name":"Olivia Newton-John Cancer Research Institute, Melbourne, Victoria, 3084Australia"},{"name":"School of Cancer Medicine, La Trobe University, Melbourne, Victoria, 3084Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amardeep S.","family":"Dhillon","sequence":"additional","affiliation":[{"name":"Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Geelong, Victoria, 3216Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9123-7684","authenticated-orcid":false,"given":"John 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