{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:15:40Z","timestamp":1780550140827,"version":"3.54.1"},"reference-count":55,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HD099174"],"award-info":[{"award-number":["R01HD099174"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["1558017"],"award-info":[{"award-number":["1558017"]}]},{"name":"National Science Foundation","award":["1757443"],"award-info":[{"award-number":["1757443"]}]},{"name":"National Science Foundation","award":["1656429"],"award-info":[{"award-number":["1656429"]}]},{"name":"National Science Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Regulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, the axolotl (Ambystoma mexicanum) regenerates missing structures through an accumulation of proliferating cells known as the blastema. To study cell division during blastema growth, we generated a transgenic line of axolotls that ubiquitously expresses a bicistronic version of the fluorescent ubiquitination-based cell-cycle indicator (FUCCI). We demonstrate near-ubiquitous FUCCI expression in developing and adult tissues, and validate these expression patterns with DNA synthesis and mitosis phase markers. We demonstrate the utility of FUCCI for live and whole-mount imaging, showing the predominantly local contribution of cells during limb and tail regeneration. We also show that spinal cord amputation results in increased proliferation at least 5\u2005mm from the site of injury. Finally, we use multimodal staining to provide cell type information for cycling cells by combining fluorescence in situ hybridization, EdU click-chemistry and immunohistochemistry on a single FUCCI tissue section. This new line of animals will be useful for studying cell cycle dynamics using in situ endpoint assays and in vivo imaging in developing and regenerating animals.<\/jats:p>","DOI":"10.1242\/dev.199637","type":"journal-article","created":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T15:32:56Z","timestamp":1646926376000},"source":"Crossref","is-referenced-by-count":10,"title":["A constitutively expressed fluorescent ubiquitination-based cell-cycle indicator (FUCCI) in axolotls for studying tissue regeneration"],"prefix":"10.1242","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4945-0294","authenticated-orcid":false,"given":"Timothy J.","family":"Duerr","sequence":"first","affiliation":[{"name":"Northeastern University, Department of Biology, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Kyung","family":"Jeon","sequence":"additional","affiliation":[{"name":"Northeastern University, Department of Biology, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaylee M.","family":"Wells","sequence":"additional","affiliation":[{"name":"University of Massachusetts Boston, Department of Biology, Boston, MA 02125, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Villanueva","sequence":"additional","affiliation":[{"name":"Northeastern University, Department of Biology, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6576-3664","authenticated-orcid":false,"given":"Ashley W.","family":"Seifert","sequence":"additional","affiliation":[{"name":"University of Kentucky, Department of Biology, Lexington, KY 40506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0127-433X","authenticated-orcid":false,"given":"Catherine D.","family":"McCusker","sequence":"additional","affiliation":[{"name":"University of Massachusetts Boston, Department of Biology, Boston, MA 02125, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6689-6108","authenticated-orcid":false,"given":"James R.","family":"Monaghan","sequence":"additional","affiliation":[{"name":"Northeastern University, Department of Biology, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"2022031717230383800_DEV199637C1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1242\/dev.084111","article-title":"Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter","volume":"140","author":"Abe","year":"2013","journal-title":"Development"},{"key":"2022031717230383800_DEV199637C2","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.yjmcc.2018.12.007","article-title":"Cardiomyocyte cell cycle dynamics and proliferation revealed through cardiac-specific transgenesis of fluorescent ubiquitinated cell cycle indicator (FUCCI)","volume":"127","author":"Alvarez","year":"2019","journal-title":"J. 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