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By doing so, the AC establishes direct contact with the invaginating primary vulF cells and attaches the developing uterus to the vulva. The signals involved and the exact sequence of events joining these two organs are not fully understood. Using a conditional let-23 EGF receptor (EGFR) allele along with novel microfluidic short- and long-term imaging methods, we discovered a specific function of the EGFR in the AC during vulval lumen morphogenesis. Tissue-specific inactivation of let-23 in the AC resulted in imprecise alignment of the AC with the primary vulval cells, delayed AC invasion and disorganized adherens junctions at the contact site forming between the AC and the dorsal vulF toroid. We propose that EGFR signaling, activated by a reciprocal EGF cue from the primary vulval cells, positions the AC at the vulval midline, guides it during invasion and assembles a cytoskeletal scaffold organizing the adherens junctions that connect the developing uterus to the dorsal vulF toroid. Thus, EGFR signaling in the AC ensures the precise alignment of the two developing organs.<\/jats:p>","DOI":"10.1242\/dev.199900","type":"journal-article","created":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T12:02:48Z","timestamp":1641297768000},"source":"Crossref","is-referenced-by-count":14,"title":["Reciprocal EGFR signaling in the anchor cell ensures precise inter-organ connection during\n                    <i>Caenorhabditis elegans<\/i>\n                    vulval morphogenesis"],"prefix":"10.1242","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7124-3056","authenticated-orcid":false,"given":"Silvan","family":"Spiri","sequence":"first","affiliation":[{"name":"Department of Molecular Life Sciences, University of Z\u00fcrich, Winterthurerstrasse 190, CH-8057 Z\u00fcrich, Switzerland"},{"name":"Molecular Life Science PhD Program, University and ETH Z\u00fcrich, CH-8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-4029","authenticated-orcid":false,"given":"Simon","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Molecular Life Sciences, University of Z\u00fcrich, Winterthurerstrasse 190, CH-8057 Z\u00fcrich, Switzerland"},{"name":"Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Z\u00fcrich, 8093 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa","family":"Mereu","sequence":"additional","affiliation":[{"name":"Department of Molecular Life Sciences, University of Z\u00fcrich, Winterthurerstrasse 190, CH-8057 Z\u00fcrich, Switzerland"},{"name":"Molecular Life Science PhD Program, University and ETH Z\u00fcrich, CH-8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1943-1356","authenticated-orcid":false,"given":"Andrew","family":"DeMello","sequence":"additional","affiliation":[{"name":"Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Z\u00fcrich, 8093 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4098-3721","authenticated-orcid":false,"given":"Alex","family":"Hajnal","sequence":"additional","affiliation":[{"name":"Department of Molecular Life Sciences, University of Z\u00fcrich, Winterthurerstrasse 190, CH-8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"2022010417023728800_DEV199900C1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1038\/348693a0","article-title":"The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily","volume":"348","author":"Aroian","year":"1990","journal-title":"Nature"},{"key":"2022010417023728800_DEV199900C2","doi-asserted-by":"publisher","first-page":"dev199674","DOI":"10.1242\/dev.199674","article-title":"Microfluidic-based imaging of complete Caenorhabditis elegans larval development","volume":"148","author":"Berger","year":"2021","journal-title":"Development"},{"key":"2022010417023728800_DEV199900C3","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1126\/science.1055642","article-title":"Notch inhibition of RAS signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development","volume":"291","author":"Berset","year":"2001","journal-title":"Science"},{"key":"2022010417023728800_DEV199900C4","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The genetics of Caenorhabditis elegans","volume":"77","author":"Brenner","year":"1974","journal-title":"Genetics"},{"key":"2022010417023728800_DEV199900C5","doi-asserted-by":"publisher","first-page":"2713","DOI":"10.1242\/dev.120.10.2713","article-title":"The lin-3\/let-23 pathway mediates inductive signalling during male spicule development in Caenorhabditis elegans","volume":"120","author":"Chamberlin","year":"1994","journal-title":"Development"},{"key":"2022010417023728800_DEV199900C6","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/S0960-9822(99)80112-2","article-title":"Reciprocal EGF signaling back to the uterus from the induced C. elegans vulva coordinates morphogenesis of epithelia","volume":"9","author":"Chang","year":"1999","journal-title":"Curr. 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