{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:02:02Z","timestamp":1790438522918,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 MH093595"],"award-info":[{"award-number":["R01 MH093595"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007747","name":"University of Utah","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007747","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Previously, we have demonstrated that a subpopulation of microglia, known as Hoxb8 microglia, is derived from the Hoxb8 lineage during the second wave (E8.5) of yolk sac hematopoiesis, whereas canonical non-Hoxb8 microglia arise from the first wave (E7.5). Hoxb8 microglia have an ontogeny distinct from non-Hoxb8 microglia. Dysfunctional Hoxb8 microglia cause the acquisition of chronic anxiety and an obsessive-compulsive spectrum-like behavior, trichotillomania, in mice. The nature and fate of the progenitors generated during E8.5 yolk sac hematopoiesis have been controversial. Herein, we use the Hoxb8 cell lineage reporter to define the ontogeny of hematopoietic cells arising during the definitive waves of hematopoiesis initiated in the E8.5 yolk sac and aorta-gonad-mesonephros (AGM) region. Our murine cell lineage analysis shows that the Hoxb8 cell lineage reporter robustly marks erythromyeloid progenitors, hematopoietic stem cells and their progeny, particularly monocytes. Hoxb8 progenitors and microglia require Myb function, a hallmark transcription factor for definitive hematopoiesis, for propagation and maturation. During adulthood, all immune lineages and, interestingly, resident macrophages in only hematopoietic\/lymphoid tissues are derived from Hoxb8 precursors. These results illustrate that the Hoxb8 lineage exclusively mirrors murine definitive hematopoiesis.<\/jats:p>","DOI":"10.1242\/dev.200200","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T09:42:52Z","timestamp":1650620572000},"source":"Crossref","is-referenced-by-count":16,"title":["Defining the\n                    <i>Hoxb8<\/i>\n                    cell lineage during murine definitive hematopoiesis"],"prefix":"10.1242","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4567-2973","authenticated-orcid":false,"given":"Donn A.","family":"Van Deren","sequence":"first","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0610-3222","authenticated-orcid":false,"given":"Shrutokirti","family":"De","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla M.","family":"Eschenbacher","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Interdepartmental Program in Neuroscience, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9591-4993","authenticated-orcid":false,"given":"Mario R.","family":"Capecchi","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"},{"name":"Interdepartmental Program in Neuroscience, University of Utah School of Medicine, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,4,22]]},"reference":[{"key":"2022042909121843100_DEV200200C1","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.cell.2010.03.055","article-title":"Hematopoietic origin of pathological grooming in Hoxb8 mutant mice","volume":"141","author":"Chen","year":"2010","journal-title":"Cell"},{"key":"2022042909121843100_DEV200200C2","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1146\/annurev.immunol.25.022106.141538","article-title":"Ontogeny of the hematopoietic system","volume":"25","author":"Cumano","year":"2007","journal-title":"Annu. 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