{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:03:45Z","timestamp":1788807825018,"version":"build-2803163510"},"reference-count":77,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100022221","name":"SickKids Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022221","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["749487"],"award-info":[{"award-number":["749487"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100019346","name":"Instituto Nacional de Cancerolog\u00eda","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100019346","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["IOP-107945"],"award-info":[{"award-number":["IOP-107945"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2016-06775"],"award-info":[{"award-number":["RGPIN-2016-06775"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RTI-2019-00361"],"award-info":[{"award-number":["RTI-2019-00361"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2022-05163"],"award-info":[{"award-number":["RGPIN-2022-05163"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in translation initiation is the binding of eukaryotic initiation factor 4E (eIF4E) to the 5\u2032 mRNA cap. In addition to canonical eIF4E-1, Drosophila has multiple eIF4E paralogs, including four (eIF4E-3, -4, -5, and -7) that are highly expressed in the testis. Among these, only eIF4E-3 has been characterized genetically. Here, using CRISPR\/Cas9 mutagenesis, we determined that eIF4E-5 is essential for male fertility. eIF4E-5 protein localizes to the distal ends of elongated spermatid cysts, and eIF4E-5 mutants exhibit defects during post-meiotic stages, including a mild defect in spermatid cyst polarization. eIF4E-5 mutants also have a fully penetrant defect in individualization, resulting in failure to produce mature sperm. Indeed, our data indicate that eIF4E-5 regulates non-apoptotic caspase activity during individualization by promoting local accumulation of the E3 ubiquitin ligase inhibitor Soti. Our results further extend the diversity of non-canonical eIF4Es that carry out distinct spatiotemporal roles during spermatogenesis.<\/jats:p>","DOI":"10.1242\/dev.200477","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T07:07:32Z","timestamp":1674630452000},"source":"Crossref","is-referenced-by-count":26,"title":["Eukaryotic translation initiation factor eIF4E-5 is required for spermiogenesis in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1780-5158","authenticated-orcid":false,"given":"Lisa","family":"Shao","sequence":"first","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 2 Department of Molecular Genetics , , 1 King's College Circle, Toronto, Ontario, M5S 1A8 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2347-0799","authenticated-orcid":false,"given":"Jaclyn M.","family":"Fingerhut","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology 3 , 455 Main Street, Cambridge, MA 02142 , USA"},{"name":"Howard Hughes Medical Institute 4 , 455 Main Street, Cambridge, MA 02142 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2324-1862","authenticated-orcid":false,"given":"Brook L.","family":"Falk","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 2 Department of Molecular Genetics , , 1 King's College Circle, Toronto, Ontario, M5S 1A8 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Han","sequence":"additional","affiliation":[{"name":"McGill University 5 Department of Biology , , 3649 Promenade Sir William Osler, Montr\u00e9al, Quebec, H3G 0B1 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9247-8746","authenticated-orcid":false,"given":"Giovanna","family":"Maldonado","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Cancerolog\u00eda (INCan) 6 Laboratory of Translation and Cancer, Unit of Biomedical Research on Cancer , , Av San Fernando 22, Mexico City 14080 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuemeng","family":"Qiao","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 7 Human Biology Program , , 300 Huron Street, Toronto, Ontario, M5S 3J6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0950-8807","authenticated-orcid":false,"given":"Vincent","family":"Lee","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 2 Department of Molecular Genetics , , 1 King's College Circle, Toronto, Ontario, M5S 1A8 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6385-1676","authenticated-orcid":false,"given":"Elizabeth","family":"Hall","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 2 Department of Molecular Genetics , , 1 King's College Circle, Toronto, Ontario, M5S 1A8 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"McGill University 5 Department of Biology , , 3649 Promenade Sir William Osler, Montr\u00e9al, Quebec, H3G 0B1 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7956-6303","authenticated-orcid":false,"given":"Gordon","family":"Polevoy","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greco","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Cancerolog\u00eda (INCan) 6 Laboratory of Translation and Cancer, Unit of Biomedical Research on Cancer , , Av San Fernando 22, Mexico City 14080 , Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4037-3501","authenticated-orcid":false,"given":"Paul","family":"Lasko","sequence":"additional","affiliation":[{"name":"McGill University 5 Department of Biology , , 3649 Promenade Sir William Osler, Montr\u00e9al, Quebec, H3G 0B1 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5925-9901","authenticated-orcid":false,"given":"Julie A.","family":"Brill","sequence":"additional","affiliation":[{"name":"The Hospital for Sick Children 1 Cell Biology Program , , PGCRL Building , 686 Bay Street, Toronto, Ontario, M5G 0A4 , Canada"},{"name":"University of Toronto 2 Department of Molecular Genetics , , 1 King's College Circle, Toronto, Ontario, M5S 1A8 , 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