{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T05:52:27Z","timestamp":1773640347805,"version":"3.50.1"},"reference-count":30,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"European Research Council"},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"name":"Ministro da Ci\u00eancia,\u00a0Tecnologia e Ensino Superior","award":["POCI-01-0145- FEDER-029129"],"award-info":[{"award-number":["POCI-01-0145- FEDER-029129"]}]},{"name":"Ministro da Ci\u00eancia,\u00a0Tecnologia e Ensino Superior","award":["PTDC\/ MED-IMU\/1416\/2020"],"award-info":[{"award-number":["PTDC\/ MED-IMU\/1416\/2020"]}]},{"name":"Ministro da Ci\u00eancia,\u00a0Tecnologia e Ensino Superior","award":["PTDC\/MED-IMU\/29129\/2017"],"award-info":[{"award-number":["PTDC\/MED-IMU\/29129\/2017"]}]},{"name":"I3S: Universidade do Porto Instituto de Investigacao e Inovacao em Saude"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The thymus stroma constitutes a fundamental microenvironment for T-cell generation. Despite the chief contribution of thymic epithelial cells, recent studies emphasize the regulatory role of mesenchymal cells in thymic function. Mesenchymal progenitors are suggested to exist in the postnatal thymus; nonetheless, an understanding of their nature and the mechanism controlling their homeostasis in vivo remains elusive. We resolved two new thymic fibroblast subsets with distinct developmental features. Whereas CD140\u03b1\u03b2+GP38+SCA-1\u2212 cells prevailed in the embryonic thymus and declined thereafter, CD140\u03b1\u03b2+GP38+SCA-1+ cells emerged in the late embryonic period and predominated in postnatal life. The fibroblastic-associated transcriptional programme was upregulated in CD140\u03b1\u03b2+GP38+SCA-1+ cells, suggesting that they represent a mature subset. Lineage analysis showed that CD140\u03b1\u03b2+GP38+SCA-1+ maintained their phenotype in thymic organoids. Strikingly, CD140\u03b1\u03b2+GP38+SCA-1\u2212 generated CD140\u03b1\u03b2+GP38+SCA-1+, inferring that this subset harboured progenitor cell activity. Moreover, the abundance of CD140\u03b1\u03b2+GP38+SCA-1+ fibroblasts was gradually reduced in Rag2\u2212\/\u2212 and Rag2\u2212\/\u2212Il2rg\u2212\/\u2212 thymi, indicating that fibroblast maturation depends on thymic crosstalk. Our findings identify CD140\u03b1\u03b2+GP38+SCA-1\u2212 as a source of fibroblast progenitors and define SCA-1 as a marker for developmental stages of thymic fibroblast differentiation.<\/jats:p>","DOI":"10.1242\/dev.200513","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T11:10:32Z","timestamp":1652958632000},"source":"Crossref","is-referenced-by-count":10,"title":["Identification of fibroblast progenitors in the developing mouse thymus"],"prefix":"10.1242","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-1099","authenticated-orcid":false,"given":"Pedro","family":"Ferreirinha","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto 1 , 4200-135, Porto , Portugal"},{"name":"Instituto de Biologia Molecular e Celular 2 , 4200-135, Porto , Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5475-4719","authenticated-orcid":false,"given":"Ruben G. 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M.","family":"Landry","sequence":"additional","affiliation":[{"name":"Genomics Core Facility, European Molecular Biology Laboratory 4 , 69117 Heidelberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1567-8389","authenticated-orcid":false,"given":"Nuno L.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto 1 , 4200-135, Porto , Portugal"},{"name":"Instituto de Biologia Molecular e Celular 2 , 4200-135, Porto , Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"2022060913464340700_DEV200513C1","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.immuni.2008.06.015","article-title":"The tumor necrosis factor family receptors RANK and CD40 cooperatively establish the thymic medullary microenvironment and self-tolerance","volume":"29","author":"Akiyama","year":"2008","journal-title":"Immunity"},{"key":"2022060913464340700_DEV200513C2","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1002\/1521-4141(2000)30:8&lt;2125::AID-IMMU2125&gt;3.0.CO;2-H","article-title":"Studies on the role of IL-7 presentation by mesenchymal fibroblasts during early thymocyte development","volume":"30","author":"Banwell","year":"2000","journal-title":"Eur. 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