{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:27:58Z","timestamp":1790720878606,"version":"4.1.0"},"reference-count":61,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T00:00:00Z","timestamp":1679270400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Several cell types have been proposed to create the required microenvironment for spermatogenesis. However, expression patterns of the key growth factors produced by these somatic cells have not been systematically studied and no such factor has been conditionally deleted from its primary source(s), raising the question of which cell type(s) are the physiological sources of these growth factors. Here, using single-cell RNA sequencing and a series of fluorescent reporter mice, we found that stem cell factor (Scf), one of the essential growth factors for spermatogenesis, was broadly expressed in testicular stromal cells, including Sertoli, endothelial, Leydig, smooth muscle and Tcf21-CreER+ stromal cells. Both undifferentiated and differentiating spermatogonia were associated with Scf-expressing Sertoli cells in the seminiferous tubule. Conditional deletion of Scf from Sertoli cells, but not any other Scf-expressing cells, blocked the differentiation of spermatogonia, leading to complete male infertility. Conditional overexpression of Scf in Sertoli cells, but not endothelial cells, significantly increased spermatogenesis. Our data reveal the importance of anatomical localization for Sertoli cells in regulating spermatogenesis and that SCF produced specifically by Sertoli cells is essential for spermatogenesis.<\/jats:p>","DOI":"10.1242\/dev.200706","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T04:35:58Z","timestamp":1677731758000},"source":"Crossref","is-referenced-by-count":26,"title":["Sertoli cells are the source of stem cell factor for spermatogenesis"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2315-6561","authenticated-orcid":false,"given":"Yi Jacky","family":"Peng","sequence":"first","affiliation":[{"name":"Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences 1 State Key Laboratory of Cell Biology , , 320 Yueyang Road, Shanghai, 200031 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu Thomas","family":"Tang","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences 1 State Key Laboratory of Cell Biology , , 320 Yueyang Road, Shanghai, 200031 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Sophie","family":"Shu","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences 1 State Key Laboratory of Cell Biology , , 320 Yueyang Road, Shanghai, 200031 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Dong","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences 1 State Key Laboratory of Cell Biology , , 320 Yueyang Road, Shanghai, 200031 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfang","family":"Shao","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine 3 , Department of Gynecology and Obstetrics , , 600 Yishan Road, Shanghai, 200233 , People's Republic of China"},{"name":"Shanghai Jiao Tong University School of Medicine-Affiliated Sixth People's Hospital 3 , Department of Gynecology and Obstetrics , , 600 Yishan Road, Shanghai, 200233 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-8799","authenticated-orcid":false,"given":"Bo O.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences 1 State Key Laboratory of Cell Biology , , 320 Yueyang Road, Shanghai, 200031 , People's Republic of China"},{"name":"Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College 2 State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem , , Tianjin, 300020 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,3,20]]},"reference":[{"key":"2023032009553905300_","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-4939-7698-0_1","article-title":"Establishment of primary culture of Sertoli cells","volume":"1748","author":"Bernardino","year":"2018","journal-title":"Methods Mol. 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