{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T08:36:46Z","timestamp":1775983006978,"version":"3.50.1"},"reference-count":48,"publisher":"The Company of Biologists","issue":"19","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 GM089782"],"award-info":[{"award-number":["R01 GM089782"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Larval terminal cells of the Drosophila tracheal system generate extensive branched tubes, requiring a huge increase in apical membrane. We discovered that terminal cells compromised for apical membrane expansion \u2013 mTOR-vATPase axis and apical polarity mutants \u2013 were invaded by the neighboring stalk cell. The invading cell grows and branches, replacing the original single intercellular junction between stalk and terminal cell with multiple intercellular junctions. Here, we characterize disjointed, a mutation in the same phenotypic class. We find that disjointed encodes Drosophila Archease, which is required for the RNA ligase (RtcB) function that is essential for tRNA maturation and for endoplasmic reticulum stress-regulated nonconventional splicing of Xbp1 mRNA. We show that the steady-state subcellular localization of Archease is principally nuclear and dependent upon TOR-vATPase activity. In tracheal cells mutant for Rheb or vATPase loci, Archease localization shifted dramatically from nucleus to cytoplasm. Further, we found that blocking tRNA maturation by knockdown of tRNAseZ also induced compensatory branching. Taken together, these data suggest that the TOR-vATPase axis promotes apical membrane growth in part through nuclear localization of Archease, where Archease is required for tRNA maturation.<\/jats:p>","DOI":"10.1242\/dev.200908","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T09:38:53Z","timestamp":1663321133000},"source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Archease by the mTOR-vATPase axis"],"prefix":"10.1242","volume":"149","author":[{"given":"Deanne","family":"Francis","sequence":"first","affiliation":[{"name":"College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, Department of Biomedicine and Molecular and Cell Biology, James Cook University 1 , Douglas, QLD 4811 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alondra S.","family":"Burguete","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Molecular and Cell Biology, The Motor Neuron Center, Columbia University Medical Center 2 , VP&S 5th floor, New York, NY 10032 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6160-3003","authenticated-orcid":false,"given":"Amin S.","family":"Ghabrial","sequence":"additional","affiliation":[{"name":"Columbia University Medical Center 3 Department of Pathology and Cell Biology , , 630 168th Street, Vagellos Physicians and Surgeons 14-401L, New York, NY 10032 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2022,10,7]]},"reference":[{"key":"2022101715410682200_DEV200908C1","doi-asserted-by":"publisher","first-page":"18711","DOI":"10.1074\/jbc.M607459200","article-title":"Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase","volume":"282","author":"Auxilien","year":"2007","journal-title":"J. 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