{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T18:55:37Z","timestamp":1784660137149,"version":"3.55.0"},"reference-count":66,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:00:00Z","timestamp":1678838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["DFG KI728\/7-2"],"award-info":[{"award-number":["DFG KI728\/7-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The murine kidney and ureter develop in a regionalized fashion from the ureteric bud and its surrounding mesenchyme. Whereas the factors that establish the metanephric cell lineages have been well characterized, much less is known about the molecular cues that specify the ureter. Here, we have identified a crucial patterning function in this process for Tbx18, a T-box transcription factor gene specifically expressed in the mesenchymal primordium of the ureter. Using misexpression and loss-of-function mice combined with molecular profiling approaches, we show that Tbx18 is required and sufficient to repress metanephric mesenchymal gene programs. We identify Wt1 as a functional target of TBX18. Our work suggests that TBX18 acts as a permissive factor in ureter specification by generating a mesenchymal domain around the distal ureteric bud where SHH and BMP4 signaling can occur.<\/jats:p>","DOI":"10.1242\/dev.201048","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T09:26:35Z","timestamp":1679649995000},"source":"Crossref","is-referenced-by-count":9,"title":["Permissive ureter specification by TBX18-mediated repression of metanephric gene expression"],"prefix":"10.1242","volume":"150","author":[{"given":"Anna-Carina","family":"Weiss","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Blank","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Bohnenpoll","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc-Jens","family":"Kleppa","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7369-0538","authenticated-orcid":false,"given":"Reginaldo","family":"Rivera-Reyes","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto Mark","family":"Taketo","sequence":"additional","affiliation":[{"name":"Kyoto University Hospital-iACT, Graduate School of Medicine, Kyoto University 2 Colon Cancer Program , , Yoshida-Honmachi, Sakyo, Kyoto 606-8501 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0011-461X","authenticated-orcid":false,"given":"Mark-Oliver","family":"Trowe","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-0257","authenticated-orcid":false,"given":"Andreas","family":"Kispert","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Hannover Medical School 1 , 30625 Hannover , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,3,23]]},"reference":[{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1172\/JCI26027","article-title":"Tbx18 regulates the development of the ureteral mesenchyme","volume":"116","author":"Airik","year":"2006","journal-title":"J. Clin. Invest."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"4918","DOI":"10.1093\/hmg\/ddq426","article-title":"Hydroureternephrosis due to loss of Sox9-regulated smooth muscle cell differentiation of the ureteric mesenchyme","volume":"19","author":"Airik","year":"2010","journal-title":"Hum. Mol. Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/0925-4773(93)90090-K","article-title":"The expression of the Wilms\u2019 tumour gene, WT1, in the developing mammalian embryo","volume":"40","author":"Armstrong","year":"1993","journal-title":"Mech. Dev."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"2564","DOI":"10.1242\/dev.122648","article-title":"Stromal Fat4 acts non-autonomously with Dchs1\/2 to restrict the nephron progenitor pool","volume":"142","author":"Bagherie-Lachidan","year":"2015","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1007\/978-1-59745-249-6_1","article-title":"The canonical Wnt\/beta-catenin signalling pathway","volume":"468","author":"Barker","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.semcdb.2014.07.014","article-title":"Ureter growth and differentiation","volume":"36","author":"Bohnenpoll","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.ydbio.2013.04.036","article-title":"Tbx18 expression demarcates multipotent precursor populations in the developing urogenital system but is exclusively required within the ureteric mesenchymal lineage to suppress a renal stromal fate","volume":"380","author":"Bohnenpoll","year":"2013","journal-title":"Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"e1006951","DOI":"10.1371\/journal.pgen.1006951","article-title":"A SHH-FOXF1-BMP4 signaling axis regulating growth and differentiation of epithelial and mesenchymal tissues in ureter development","volume":"13","author":"Bohnenpoll","year":"2017","journal-title":"PLoS Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1967","DOI":"10.1242\/dev.004234","article-title":"Tailbud-derived mesenchyme promotes urinary tract segmentation via BMP4 signaling","volume":"134","author":"Brenner-Anantharam","year":"2007","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"3093","DOI":"10.1242\/dev.110601","article-title":"Single cell dissection of early kidney development: multilineage priming","volume":"141","author":"Brunskill","year":"2014","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.1101\/gad.300104","article-title":"The T-box transcription factor Tbx18 maintains the separation of anterior and posterior somite compartments","volume":"18","author":"Bussen","year":"2004","journal-title":"Genes Dev."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.devcel.2005.05.016","article-title":"Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system","volume":"9","author":"Carroll","year":"2005","journal-title":"Dev. Cell"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1161\/01.RES.0000227571.84189.65","article-title":"Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18","volume":"98","author":"Christoffels","year":"2006","journal-title":"Circ. Res."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1002\/bies.20357","article-title":"GDNF\/Ret signaling and the development of the kidney","volume":"28","author":"Costantini","year":"2006","journal-title":"BioEssays"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","DOI":"10.3824\/stembook.1.34.1","article-title":"Mouse kidney development","volume-title":"StemBook","author":"Davidson","year":"2008"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"dev190108","DOI":"10.1242\/dev.190108","article-title":"Identification and characterization of cellular heterogeneity within the developing renal interstitium","volume":"147","author":"England","year":"2020","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.devcel.2011.07.014","article-title":"A wt1-controlled chromatin switching mechanism underpins tissue-specific wnt4 activation and repression","volume":"21","author":"Essafi","year":"2011","journal-title":"Dev. Cell"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"25748","DOI":"10.1074\/jbc.M703724200","article-title":"Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors","volume":"282","author":"Farin","year":"2007","journal-title":"J. Biol. Chem."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"4986","DOI":"10.1101\/pdb.prot4986","article-title":"Hematoxylin and eosin staining of tissue and cell sections","volume":"3","author":"Fischer","year":"2008","journal-title":"CSH Protoc"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1038\/nm.3142","article-title":"Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1","volume":"19","author":"Gebeshuber","year":"2013","journal-title":"Nat. Med."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1093\/cvr\/cvs277","article-title":"Tbx18 function in epicardial development","volume":"96","author":"Greulich","year":"2012","journal-title":"Cardiovasc. Res."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1002\/jez.1401300207","article-title":"Inductive interaction in the development of the mouse metanephros","volume":"130","author":"Grobstein","year":"1955","journal-title":"J. Exp. Zool."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1093\/hmg\/11.6.651","article-title":"WT1 is a key regulator of podocyte function: reduced expression levels cause crescentic glomerulonephritis and mesangial sclerosis","volume":"11","author":"Guo","year":"2002","journal-title":"Hum. Mol. Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"5931","DOI":"10.1093\/emboj\/18.21.5931","article-title":"Intestinal polyposis in mice with a dominant stable mutation of the beta-catenin gene","volume":"18","author":"Harada","year":"1999","journal-title":"EMBO J."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1101\/gad.938601","article-title":"Hedgehog signaling in animal development: paradigms and principles","volume":"15","author":"Ingham","year":"2001","journal-title":"Genes Dev."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1128\/MCB.22.4.1172-1183.2002","article-title":"Wnt\/beta-catenin\/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway","volume":"22","author":"Jho","year":"2002","journal-title":"Mol. Cell. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1242\/dev.057646","article-title":"Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development","volume":"138","author":"Karner","year":"2011","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"4225","DOI":"10.1242\/dev.125.21.4225","article-title":"Wnt-4 is a mesenchymal signal for epithelial transformation of metanephric mesenchyme in the developing kidney","volume":"125","author":"Kispert","year":"1998","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.stem.2008.05.020","article-title":"Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development","volume":"3","author":"Kobayashi","year":"2008","journal-title":"Cell Stem Cell"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1016\/j.stemcr.2014.08.008","article-title":"Identification of a multipotent self-renewing stromal progenitor population during mammalian kidney organogenesis","volume":"3","author":"Kobayashi","year":"2014","journal-title":"Stem Cell Rep."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/0092-8674(93)90515-R","article-title":"WT-1 is required for early kidney development","volume":"74","author":"Kreidberg","year":"1993","journal-title":"Cell"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1002\/(SICI)1526-968X(200002)26:2&lt;162::AID-GENE21&gt;3.0.CO;2-R","article-title":"Neural crest expression of Cre recombinase directed by the proximal Pax3 promoter in transgenic mice","volume":"26","author":"Li","year":"2000","journal-title":"Genesis"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1038\/nature02083","article-title":"Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis","volume":"426","author":"Li","year":"2004","journal-title":"Nature"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"a008300","DOI":"10.1101\/cshperspect.a008300","article-title":"Mammalian kidney development: principles, progress, and projections","volume":"4","author":"Little","year":"2012","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1042\/BCJ20210642","article-title":"Proteomic analysis identifies ZMYM2 as endogenous binding partner of TBX18 protein in 293 and A549 cells","volume":"479","author":"L\u00fcdtke","year":"2022","journal-title":"Biochem. J."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.ydbio.2017.11.006","article-title":"Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf","volume":"434","author":"Magella","year":"2018","journal-title":"Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"3553","DOI":"10.1093\/hmg\/ddx242","article-title":"BMP4 uses several different effector pathways to regulate proliferation and differentiation in the epithelial and mesenchymal tissue compartments of the developing mouse ureter","volume":"26","author":"Mamo","year":"2017","journal-title":"Hum. Mol. Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/bs.ctdb.2015.10.010","article-title":"Development of the mammalian kidney","volume":"117","author":"McMahon","year":"2017","journal-title":"Curr. Top. Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"14824","DOI":"10.1038\/s41598-017-14809-8","article-title":"Asymmetric BMP4 signalling improves the realism of kidney organoids","volume":"7","author":"Mills","year":"2017","journal-title":"Sci. Rep."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1177\/002215540104900101","article-title":"Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol","volume":"49","author":"Moorman","year":"2001","journal-title":"J. Histochem. Cytochem."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"4444","DOI":"10.1038\/ncomms5444","article-title":"WT1 controls antagonistic FGF and BMP-pSMAD pathways in early renal progenitors","volume":"5","author":"Motamedi","year":"2014","journal-title":"Nat. Commun."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.ydbio.2008.09.010","article-title":"Osr1 expression demarcates a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney","volume":"324","author":"Mugford","year":"2008","journal-title":"Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1002\/dvg.20335","article-title":"A global double-fluorescent Cre reporter mouse","volume":"45","author":"Muzumdar","year":"2007","journal-title":"Genesis"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"dev199664","DOI":"10.1242\/dev.199664","article-title":"Deep learning is widely applicable to phenotyping embryonic development and disease","volume":"148","author":"Naert","year":"2022","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"e1007181","DOI":"10.1371\/journal.pgen.1007181","article-title":"Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies","volume":"14","author":"O'Brien","year":"2018","journal-title":"PLoS Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"2533","DOI":"10.1242\/dev.006155","article-title":"Wnt\/\u03b2-catenin signaling regulates nephron induction during mouse kidney development","volume":"134","author":"Park","year":"2007","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.ydbio.2005.12.034","article-title":"Role of fibroblast growth factor receptors 1 and 2 in the metanephric mesenchyme","volume":"291","author":"Poladia","year":"2006","journal-title":"Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1242\/dev.035592","article-title":"Interplay between activin and Hox genes determines the formation of the kidney morphogenetic field","volume":"136","author":"Preger-Ben Noon","year":"2009","journal-title":"Development. Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"e0200964","DOI":"10.1371\/journal.pone.0200964","article-title":"Proteomic analysis identifies transcriptional cofactors and homeobox transcription factors as TBX18 binding proteins","volume":"13","author":"Rivera-Reyes","year":"2018","journal-title":"PLoS ONE"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1016\/S0960-9822(01)00143-9","article-title":"Zebrafish pea3 and erm are general targets of FGF8 signaling","volume":"11","author":"Roehl","year":"2001","journal-title":"Curr. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511565083","volume-title":"Organogenesis of the Kidney","author":"Saxen","year":"1987"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"5214","DOI":"10.1038\/sj.emboj.7601381","article-title":"Six2 is required for suppression of nephrogenesis and progenitor renewal in the developing kidney","volume":"25","author":"Self","year":"2006","journal-title":"EMBO J."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"5522","DOI":"10.1073\/pnas.96.10.5522","article-title":"The cyclin D1 gene is a target of the \u03b2-catenin\/LEF-1 pathway","volume":"96","author":"Shtutman","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/S0925-4773(99)00058-1","article-title":"Cloning and expression analysis of a mouse gene related to Drosophila odd-skipped","volume":"84","author":"So","year":"1999","journal-title":"Mech. Dev."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1002\/(SICI)1520-6408(1999)24:3\/4&lt;241::AID-DVG7&gt;3.0.CO;2-R","article-title":"Expression of green fluorescent protein in the ureteric bud of transgenic mice: a new tool for the analysis of ureteric bud morphogenesis","volume":"24","author":"Srinivas","year":"1999","journal-title":"Dev. Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"3247","DOI":"10.1242\/dev.023176","article-title":"Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development","volume":"135","author":"ten Berge","year":"2008","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1016\/j.ydbio.2006.08.063","article-title":"Odd-skipped genes encode repressors that control kidney development","volume":"301","author":"Tena","year":"2007","journal-title":"Dev. Biol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"3099","DOI":"10.1242\/dev.077388","article-title":"Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter","volume":"139","author":"Trowe","year":"2012","journal-title":"Development"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"e53895","DOI":"10.7554\/eLife.53895","article-title":"R-spondin signalling is essential for the maintenance and differentiation of mouse nephron progenitors","volume":"9","author":"Vidal","year":"2020","journal-title":"eLife"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.ajhg.2015.07.001","article-title":"Mutations in TBX18 cause dominant urinary tract malformations via transcriptional dysregulation of ureter development","volume":"97","author":"Vivante","year":"2015","journal-title":"Am. J. Hum. Genet."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1016\/j.juro.2008.08.117","article-title":"Antagonism of BMP4 signaling disrupts smooth muscle investment of the ureter and ureteropelvic junction","volume":"181","author":"Wang","year":"2009","journal-title":"J. Urol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1002\/path.5518","article-title":"Expansion of the renal capsular stroma, ureteric bud branching defects and cryptorchidism in mice with Wilms tumor 1 gene deletion in the stromal compartment of the developing kidney","volume":"252","author":"Weiss","year":"2020","journal-title":"J. Pathol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1101\/gad.993302","article-title":"Hox11 paralogous genes are essential for metanephric kidney induction","volume":"16","author":"Wellik","year":"2002","journal-title":"Genes Dev."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/0076-6879(93)25025-W","article-title":"Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts","volume":"225","author":"Wilkinson","year":"1993","journal-title":"Methods Enzymol."},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1016\/j.devcel.2014.10.015","article-title":"Eya1 interacts with Six2 and Myc to regulate expansion of the nephron progenitor pool during nephrogenesis","volume":"31","author":"Xu","year":"2014","journal-title":"Dev. Cell"},{"key":"2023032409255988000_","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.1242\/dev.129.22.5301","article-title":"Sonic hedgehog regulates proliferation and differentiation of mesenchymal cells in the mouse metanephric kidney","volume":"129","author":"Yu","year":"2002","journal-title":"Development"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.201048\/2647837\/dev201048.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.201048\/2647837\/dev201048.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T14:57:33Z","timestamp":1702133853000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/150\/6\/dev201048\/297298\/Permissive-ureter-specification-by-TBX18-mediated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,15]]},"references-count":66,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,3,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.201048","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,15]]},"article-number":"dev201048"}}