{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T18:45:52Z","timestamp":1784486752815,"version":"3.55.0"},"reference-count":78,"publisher":"The Company of Biologists","issue":"21","license":[{"start":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T00:00:00Z","timestamp":1667174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM131746"],"award-info":[{"award-number":["R35GM131746"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["T32GM008798"],"award-info":[{"award-number":["T32GM008798"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["F31GM126741"],"award-info":[{"award-number":["F31GM126741"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Caenorhabditis elegans larvae display developmental plasticity in response to environmental conditions: in adverse conditions, second-stage larvae enter a reversible, long-lived dauer stage instead of proceeding to reproductive adulthood. Dauer entry interrupts vulval induction and is associated with a reprogramming-like event that preserves the multipotency of vulval precursor cells (VPCs), allowing vulval development to reinitiate if conditions improve. Vulval induction requires the LIN-3\/EGF-like signal from the gonad, which activates EGFR-Ras-ERK signal transduction in the nearest VPC, P6.p. Here, using a biosensor and live imaging we show that EGFR-Ras-ERK activity is downregulated in P6.p in dauers. We investigated this process using gene mutations or transgenes to manipulate different steps of the pathway, and by analyzing LET-23\/EGFR subcellular localization during dauer life history. We found that the response to EGF is attenuated at or upstream of Ras activation, and discuss potential membrane-associated mechanisms that could achieve this. We also describe other findings pertaining to the maintenance of VPC competence and quiescence in dauer larvae. Our analysis indicates that VPCs have L2-like and unique dauer stage features rather than features of L3 VPCs in continuous development.<\/jats:p>","DOI":"10.1242\/dev.201094","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T15:30:05Z","timestamp":1665675005000},"source":"Crossref","is-referenced-by-count":6,"title":["EGFR signal transduction is downregulated in <i>C. elegans<\/i> vulval precursor cells during dauer diapause"],"prefix":"10.1242","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0160-7747","authenticated-orcid":false,"given":"Catherine","family":"O'Keeffe","sequence":"first","affiliation":[{"name":"Columbia University Department of Biological Sciences , , New York, NY 10027 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7180-3706","authenticated-orcid":false,"given":"Iva","family":"Greenwald","sequence":"additional","affiliation":[{"name":"Columbia University Department of Biological Sciences , , New York, NY 10027 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"2022103110413734900_DEV201094C1","doi-asserted-by":"publisher","first-page":"3545","DOI":"10.1242\/dev.068148","article-title":"Notch and Ras promote sequential steps of excretory tube development in C. elegans","volume":"138","author":"Abdus-Saboor","year":"2011","journal-title":"Development"},{"key":"2022103110413734900_DEV201094C2","doi-asserted-by":"publisher","first-page":"3094","DOI":"10.1016\/j.cub.2019.07.062","article-title":"HLH-2\/E2A expression links stochastic and deterministic elements of a cell fate decision during C. elegans gonadogenesis","volume":"29","author":"Attner","year":"2019","journal-title":"Curr. 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