{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:10:08Z","timestamp":1791378608277,"version":"4.3.2"},"reference-count":41,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T00:00:00Z","timestamp":1681516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["FWO G075222"],"award-info":[{"award-number":["FWO G075222"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["G142"],"award-info":[{"award-number":["G142"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["221856\/Z\/20\/Z"],"award-info":[{"award-number":["221856\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["221856\/Z\/20\/Z"],"award-info":[{"award-number":["221856\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Our understanding of the molecular events driving cell specification in early mammalian development relies mainly on mouse studies, and it remains unclear whether these mechanisms are conserved across mammals, including humans. We have shown that the establishment of cell polarity via aPKC is a conserved event in the initiation of the trophectoderm (TE) placental programme in mouse, cow and human embryos. However, the mechanisms transducing cell polarity into cell fate in cow and human embryos are unknown. Here, we have examined the evolutionary conservation of Hippo signalling, which is thought to function downstream of aPKC activity, in four different mammalian species: mouse, rat, cow and human. In all four species, inhibition of the Hippo pathway by targeting LATS kinases is sufficient to drive ectopic TE initiation and downregulation of SOX2. However, the timing and localisation of molecular markers differ across species, with rat embryos more closely recapitulating human and cow developmental dynamics, compared with the mouse. Our comparative embryology approach uncovered intriguing differences as well as similarities in a fundamental developmental process among mammals, reinforcing the importance of cross-species investigations.<\/jats:p>","DOI":"10.1242\/dev.201112","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T13:26:21Z","timestamp":1679923581000},"source":"Crossref","is-referenced-by-count":45,"title":["A conserved role of the Hippo signalling pathway in initiation of the first lineage specification event across mammals"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-3820","authenticated-orcid":false,"given":"Claudia","family":"Gerri","sequence":"first","affiliation":[{"name":"The Francis Crick Institute 1 Human Embryo and Stem Cell Laboratory , , 1 Midland Road, London NW1 1AT , 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4 Department of Reproduction and Immunology , , 1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Brumm","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute 1 Human Embryo and Stem Cell Laboratory , , 1 Midland Road, London NW1 1AT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9614-1403","authenticated-orcid":false,"given":"Claire S.","family":"Simon","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute 1 Human Embryo and Stem Cell Laboratory , , 1 Midland Road, London NW1 1AT , UK"},{"name":"The Centre for Trophoblast Research 5 , Department of Physiology, Development and Neuroscience , , Cambridge CB2 3EG , UK"},{"name":"University of Cambridge 5 , Department of Physiology, Development and Neuroscience , , Cambridge CB2 3EG , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Lee","sequence":"additional","affiliation":[{"name":"Hewitt Fertility Centre, Liverpool Women's Hospital 6 , Liverpool, L8 7SS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Montesinos","sequence":"additional","affiliation":[{"name":"Hewitt Fertility Centre, Liverpool Women's Hospital 6 , Liverpool, L8 7SS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Hassitt","sequence":"additional","affiliation":[{"name":"Hewitt Fertility Centre, Liverpool Women's Hospital 6 , Liverpool, L8 7SS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hockenhull","sequence":"additional","affiliation":[{"name":"Hewitt Fertility Centre, Liverpool Women's Hospital 6 , Liverpool, L8 7SS , 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A.","family":"Fouladi-Nashta","sequence":"additional","affiliation":[{"name":"Royal Veterinary College 2 Department of Comparative Biomedical Sciences , , Hawkshead Campus, Potters Bar AL9 7TA , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde","family":"Van de Velde","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel 4 Department of Reproduction and Immunology , , 1090 Brussels, Belgium"},{"name":"Brussels IVF, UZ-Brussel 8 , 1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-4734","authenticated-orcid":false,"given":"Kathy K.","family":"Niakan","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute 1 Human Embryo and Stem Cell Laboratory , , 1 Midland Road, London NW1 1AT , UK"},{"name":"The Centre for Trophoblast Research 5 , Department of Physiology, Development and Neuroscience , , Cambridge CB2 3EG , UK"},{"name":"University of Cambridge 5 , Department of Physiology, Development and Neuroscience , , Cambridge CB2 3EG , UK"},{"name":"Wellcome Trust \u2013 Medical Research Council Stem Cell Institute, University of Cambridge, Jeffrey Cheah Biomedical Centre 9 , Puddicombe Way, Cambridge CB2 0AW , UK"},{"name":"Babraham Institute 10 Epigenetics Programme , , Cambridge CB22 3AT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"2023042008094359000_DEV201112C1","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1095\/biolreprod.110.084400","article-title":"Cell polarity regulator PARD6B is essential for trophectoderm formation in the preimplantation mouse embryo","volume":"83","author":"Alarcon","year":"2010","journal-title":"Biol. 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