{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:52:50Z","timestamp":1781333570496,"version":"3.54.1"},"reference-count":65,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["APP1138014"],"award-info":[{"award-number":["APP1138014"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["APP1180929"],"award-info":[{"award-number":["APP1180929"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["APP1182330"],"award-info":[{"award-number":["APP1182330"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015539","name":"Australian Government","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015539","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["5U01HG007530-08"],"award-info":[{"award-number":["5U01HG007530-08"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001782","name":"University of Melbourne","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001782","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The development and function of male gametes is dependent on a dynamic microtubule network, yet how this is regulated remains poorly understood. We have recently shown that microtubule severing, via the action of the meiotic AAA ATPase protein clade, plays a crucial role in this process. Here, we sought to elucidate the roles of spastin, an as-yet-unexplored member of this clade in spermatogenesis. Using a SpastKO\/KO mouse model, we reveal that spastin loss resulted in a complete loss of functional germ cells. Spastin plays a crucial role in the assembly and function of the male meiotic spindle. Consistent with meiotic failure, round spermatid nuclei were enlarged, indicating aneuploidy, but were still able to enter spermiogenesis. During spermiogenesis, we observed extreme abnormalities in manchette structure, acrosome biogenesis and, commonly, a catastrophic loss of nuclear integrity. This work defines an essential role for spastin in regulating microtubule dynamics during spermatogenesis, and is of potential relevance to individuals carrying spastin variants and to the medically assisted reproductive technology industry.<\/jats:p>","DOI":"10.1242\/dev.201183","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T11:56:31Z","timestamp":1679918191000},"source":"Crossref","is-referenced-by-count":7,"title":["Spastin is an essential regulator of male meiosis, acrosome formation, manchette structure and nuclear integrity"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4588-3455","authenticated-orcid":false,"given":"Samuel R.","family":"Cheers","sequence":"first","affiliation":[{"name":"School of BioSciences and Bio21 Institute, The University of Melbourne 1 , Parkville, VIC 3010 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9334-4256","authenticated-orcid":false,"given":"Anne E.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"School of BioSciences and Bio21 Institute, The University of Melbourne 1 , Parkville, VIC 3010 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6846-9313","authenticated-orcid":false,"given":"Travis K.","family":"Johnson","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Monash University 2 , Clayton, VIC 3800 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Jo","family":"Merriner","sequence":"additional","affiliation":[{"name":"School of BioSciences and Bio21 Institute, The University of Melbourne 1 , Parkville, VIC 3010 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moira K.","family":"O'Bryan","sequence":"additional","affiliation":[{"name":"School of BioSciences and Bio21 Institute, The University of Melbourne 1 , Parkville, VIC 3010 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2009-0165","authenticated-orcid":false,"given":"Jessica E. M.","family":"Dunleavy","sequence":"additional","affiliation":[{"name":"School of BioSciences and Bio21 Institute, The University of Melbourne 1 , Parkville, VIC 3010 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"2023033011310406300_","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1083\/jcb.145.2.305","article-title":"An essential role for katanin in severing microtubules in the neuron","volume":"145","author":"Ahmad","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023033011310406300_","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1083\/jcb.201211045","article-title":"An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome","volume":"202","author":"Allison","year":"2013","journal-title":"J. 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