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N-WASP tightly controls actin polymerization dynamics by activating an actin nucleator Arp2\/3. However, the importance of N-WASP-Arp2\/3 signaling in the assembly of neurite architecture in vivo has not been clarified. Here, we demonstrate that N-WASP-Arp2\/3 signaling plays a crucial role in the maturation of cerebellar Purkinje cell (PC) dendrites in vivo in mice. N-WASP was expressed and activated in developing PCs. Inhibition of Arp2\/3 and N-WASP from the beginning of dendrite formation severely disrupted the establishment of a single stem dendrite, which is a characteristic basic structure of PC dendrites. Inhibition of Arp2\/3 after stem dendrite formation resulted in hypoplasia of the PC dendritic tree. Cdc42, an upstream activator of N-WASP, is required for N-WASP-Arp2\/3 signaling-mediated PC dendrite maturation. In addition, overactivation of N-WASP is also detrimental to dendrite formation in PCs. These findings reveal that proper activation of N-WASP-Arp2\/3 signaling is crucial for multiple steps of PC dendrite maturation in vivo.<\/jats:p>","DOI":"10.1242\/dev.201214","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T16:09:12Z","timestamp":1670256552000},"source":"Crossref","is-referenced-by-count":13,"title":["N-WASP-Arp2\/3 signaling controls multiple steps of dendrite maturation in Purkinje cells <i>in vivo<\/i>"],"prefix":"10.1242","volume":"149","author":[{"given":"Koichi","family":"Hasegawa","sequence":"first","affiliation":[{"name":"School of Medicine, Shimane University Department of Neural and Muscular Physiology , , 89-1 Enya-cho, Izumo-shi, Shimane 693-8501 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi K.","family":"Matsui","sequence":"additional","affiliation":[{"name":"School of Medicine, Shimane University Department of Neural and Muscular Physiology , , 89-1 Enya-cho, Izumo-shi, Shimane 693-8501 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junpei","family":"Kondo","sequence":"additional","affiliation":[{"name":"School of Medicine, Shimane University Department of Neural and Muscular Physiology , , 89-1 Enya-cho, Izumo-shi, Shimane 693-8501 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-3035","authenticated-orcid":false,"given":"Ken-ichiro","family":"Kuwako","sequence":"additional","affiliation":[{"name":"School of Medicine, Shimane University Department of Neural and Muscular Physiology , , 89-1 Enya-cho, Izumo-shi, Shimane 693-8501 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,12,5]]},"reference":[{"key":"2022120516090244400_DEV201214C1","doi-asserted-by":"publisher","first-page":"4181","DOI":"10.1038\/ncomms5181","article-title":"Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation","volume":"5","author":"Alvarez-Saavedra","year":"2014","journal-title":"Nat. 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