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Here, we have characterized the function of a testis-specific IQ motif-containing H gene (Iqch) using a mutant mouse model. We found that Iqch is essential for the specific expression of RNA isoforms during spermatogenesis. Using immunohistochemistry of the testis, we noted that Iqch was expressed mainly in the nucleus of spermatocyte and spermatid, where IQCH appeared juxtaposed with SRRM2 and ERSP1 in the nuclear speckles, suggesting that interactions among these proteins regulate alternative splicing (AS). Using RNA-seq, we found that mutant Iqch produces alterations in gene expression, including the clear downregulation of testis-specific lncRNAs and protein-coding genes at the spermatid stage, and AS modifications \u2013 principally increased intron retention \u2013 resulting in complete male infertility. Interestingly, we identified previously unreported spliced transcripts in the wild-type testis, while mutant Iqch modified the expression and use of hundreds of RNA isoforms, favouring the expression of the canonical form. This suggests that Iqch is part of a splicing control mechanism, which is essential in germ cell biology.<\/jats:p>","DOI":"10.1242\/dev.201334","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T13:10:34Z","timestamp":1680009034000},"source":"Crossref","is-referenced-by-count":3,"title":["An ancient testis-specific IQ motif-containing H gene regulates specific transcript isoform expression during spermatogenesis"],"prefix":"10.1242","volume":"150","author":[{"given":"Paula","family":"Navarrete-L\u00f3pez","sequence":"first","affiliation":[{"name":"INIA-CSIC 1 Departamento de Reproducci\u00f3n Animal , , Avenida Puerta de Hierro 12. 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Local 10, 28040 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Parra","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid 2 Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda , , 28049 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Viera","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid 2 Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda , , 28049 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 \u00c1ngel","family":"Suja","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid 2 Unidad de Biolog\u00eda Celular, Departamento de Biolog\u00eda , , 28049 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9893-9179","authenticated-orcid":false,"given":"Alfonso","family":"Guti\u00e9rrez-Ad\u00e1n","sequence":"additional","affiliation":[{"name":"INIA-CSIC 1 Departamento de Reproducci\u00f3n Animal , , Avenida Puerta de Hierro 12. 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