{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:30:44Z","timestamp":1790343044396,"version":"4.1.0"},"reference-count":60,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"name":"Gates Center for Regenerative Medicine"},{"DOI":"10.13039\/100014450","name":"University of Colorado Anschutz Medical Campus","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014450","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Taste buds on the tongue contain taste receptor cells (TRCs) that detect sweet, sour, salty, umami and bitter stimuli. Like non-taste lingual epithelium, TRCs are renewed from basal keratinocytes, many of which express the transcription factor SOX2. Genetic lineage tracing has shown that SOX2+ lingual progenitors give rise to both taste and non-taste lingual epithelium in the posterior circumvallate taste papilla (CVP) of mice. However, SOX2 is variably expressed among CVP epithelial cells, suggesting that their progenitor potential may vary. Using transcriptome analysis and organoid technology, we show that cells expressing SOX2 at higher levels are taste-competent progenitors that give rise to organoids comprising both TRCs and lingual epithelium. Conversely, organoids derived from progenitors that express SOX2 at lower levels are composed entirely of non-taste cells. Hedgehog and WNT\/\u03b2-catenin are required for taste homeostasis in adult mice. However, manipulation of hedgehog signaling in organoids has no impact on TRC differentiation or progenitor proliferation. By contrast, WNT\/\u03b2-catenin promotes TRC differentiation in vitro in organoids derived from higher but not low SOX2+ expressing progenitors.<\/jats:p>","DOI":"10.1242\/dev.201375","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T07:53:58Z","timestamp":1676534038000},"source":"Crossref","is-referenced-by-count":20,"title":["High\n                    <i>Sox2<\/i>\n                    expression predicts taste lineage competency of lingual progenitors\n                    <i>in vitro<\/i>"],"prefix":"10.1242","volume":"150","author":[{"given":"Lauren A.","family":"Shechtman","sequence":"first","affiliation":[{"name":"University of Colorado Anschutz Medical Campus 1 Department of Cell and Developmental Biology , , Aurora, CO 80045 , USA"},{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer K.","family":"Scott","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus 1 Department of Cell and Developmental Biology , , Aurora, CO 80045 , USA"},{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8346-1464","authenticated-orcid":false,"given":"Eric D.","family":"Larson","sequence":"additional","affiliation":[{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"},{"name":"University of Colorado Anschutz Medical Campus 3 Department of Otolaryngology , , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor J.","family":"Isner","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus 1 Department of Cell and Developmental Biology , , Aurora, CO 80045 , USA"},{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"},{"name":"Cell Biology, Stem Cells and Development Graduate Program, University of Colorado Anschutz Medical Campus 4 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Cell Biology, Stem Cells and Development Graduate Program, University of Colorado Anschutz Medical Campus 4 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dany","family":"Gaillard","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus 1 Department of Cell and Developmental Biology , , Aurora, CO 80045 , USA"},{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2334-7344","authenticated-orcid":false,"given":"Peter J.","family":"Dempsey","sequence":"additional","affiliation":[{"name":"Cell Biology, Stem Cells and Development Graduate Program, University of Colorado Anschutz Medical Campus 4 , Aurora, CO 80045 , USA"},{"name":"University of Colorado Anschutz Medical Campus 5 Section of Developmental Biology, Department of Pediatrics , , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7998-2219","authenticated-orcid":false,"given":"Linda A.","family":"Barlow","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus 1 Department of Cell and Developmental Biology , , Aurora, CO 80045 , USA"},{"name":"Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus 2 , Aurora, CO 80045 , USA"},{"name":"Cell Biology, Stem Cells and Development Graduate Program, University of Colorado Anschutz Medical Campus 4 , Aurora, CO 80045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"2023021612535306400_","first-page":"1","article-title":"Discovery of potent and highly selective inhibitors of GSK3b","volume-title":"Probe Reports from the NIH Molecular Libraries Program","author":"An","year":"2010"},{"key":"2023021612535306400_","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.stem.2011.09.001","article-title":"Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice","volume":"9","author":"Arnold","year":"2011","journal-title":"Cell Stem Cell"},{"key":"2023021612535306400_","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/S0091-679X(04)74025-X","article-title":"Using reporter genes to study cis-regulatory 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