{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:17:38Z","timestamp":1791260258575,"version":"4.1.0"},"reference-count":66,"publisher":"The Company of Biologists","issue":"5","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001779","name":"Monash University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001779","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>During gonadal sex determination, the supporting cell lineage differentiates into Sertoli cells in males and pre-granulosa cells in females. Recently, single cell RNA-seq data have indicated that chicken steroidogenic cells are derived from differentiated supporting cells. This differentiation process is achieved by a sequential upregulation of steroidogenic genes and downregulation of supporting cell markers. The exact mechanism regulating this differentiation process remains unknown. We have identified TOX3 as a previously unreported transcription factor expressed in embryonic Sertoli cells of the chicken testis. TOX3 knockdown in males resulted in increased CYP17A1-positive Leydig cells. TOX3 overexpression in male and female gonads resulted in a significant decline in CYP17A1-positive steroidogenic cells. In ovo knockdown of the testis determinant DMRT1 in male gonads resulted in a downregulation of TOX3 expression. Conversely, DMRT1 overexpression caused an increase in TOX3 expression. Taken together, these data indicate that DMRT1-mediated regulation of TOX3 modulates expansion of the steroidogenic lineage, either directly, via cell lineage allocation, or indirectly, via signaling from the supporting to steroidogenic cell populations.<\/jats:p>","DOI":"10.1242\/dev.201466","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T06:43:05Z","timestamp":1676529785000},"source":"Crossref","is-referenced-by-count":7,"title":["DMRT1-mediated regulation of\n                    <i>TOX3<\/i>\n                    modulates expansion of the gonadal steroidogenic cell lineage in the chicken embryo"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-2720","authenticated-orcid":false,"given":"Martin A.","family":"Estermann","sequence":"first","affiliation":[{"name":"Monash Biomedicine Discovery Institute, Monash University Department of Anatomy and Developmental Biology , , Clayton, VIC 3800 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9036-6652","authenticated-orcid":false,"given":"Andrew T.","family":"Major","sequence":"additional","affiliation":[{"name":"Monash Biomedicine Discovery Institute, Monash University Department of Anatomy and Developmental Biology , , Clayton, VIC 3800 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1670-5545","authenticated-orcid":false,"given":"Craig A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Monash Biomedicine Discovery Institute, Monash University Department of Anatomy and Developmental Biology , , Clayton, VIC 3800 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"2023030920242034500_","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1186\/s12864-015-1886-5","article-title":"Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq","volume":"16","author":"Ayers","year":"2015","journal-title":"BMC Genomics"},{"key":"2023030920242034500_","doi-asserted-by":"publisher","first-page":"812","DOI":"10.6026\/97320630015812","article-title":"Genotype based risk predictors for polycystic ovary syndrome in western Saudi Arabia","volume":"15","author":"Bakhashab","year":"2019","journal-title":"Bioinformation"},{"key":"2023030920242034500_","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.ydbio.2004.11.030","article-title":"Delayed Sry and Sox9 expression in developing mouse gonads underlies B6-Y(DOM) sex reversal","volume":"278","author":"Bullejos","year":"2005","journal-title":"Dev. 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