{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T23:23:43Z","timestamp":1787268223722,"version":"build-2736575974"},"reference-count":67,"publisher":"The Company of Biologists","issue":"21","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SCHO 503\/27-1"],"award-info":[{"award-number":["SCHO 503\/27-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Actin-related proteins (Arps) are classified according to their similarity to actin and are involved in diverse cellular processes. ACTL7B is a testis-specific Arp, and is highly conserved in rodents and primates. ACTL7B is specifically expressed in round and elongating spermatids during spermiogenesis. Here, we have generated an Actl7b-null allele in mice to unravel the role of ACTL7B in sperm formation. Male mice homozygous for the Actl7b-null allele (Actl7b\u2212\/\u2212) were infertile, whereas heterozygous males (Actl7b+\/\u2212) were fertile. Severe spermatid defects, such as detached acrosomes, disrupted membranes and flagella malformations start to appear after spermiogenesis step 9 in Actl7b\u2212\/\u2212 mice, finally resulting in spermatogenic arrest. Abnormal spermatids were degraded and levels of autophagy markers were increased. Co-immunoprecipitation with mass spectrometry experiments identified an interaction between ACTL7B and the LC8 dynein light chains DYNLL1 and DYNLL2, which are first detected in step 9 spermatids and mislocalized when ACTL7B is absent. Our data unequivocally establish that mutations in ACTL7B are directly related to male infertility, pressing for additional research in humans.<\/jats:p>","DOI":"10.1242\/dev.201593","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T04:31:33Z","timestamp":1696566693000},"source":"Crossref","is-referenced-by-count":11,"title":["<i>Actl7b<\/i>\n                    deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5805-965X","authenticated-orcid":false,"given":"Gina E.","family":"Merges","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn 1 Department of Developmental Pathology , , 53127 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0681-0392","authenticated-orcid":false,"given":"Lena","family":"Ar\u00e9valo","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn 1 Department of Developmental Pathology , , 53127 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7719-2467","authenticated-orcid":false,"given":"Andjela","family":"Kovacevic","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn 1 Department of Developmental Pathology , , 53127 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keerthika","family":"Lohanadan","sequence":"additional","affiliation":[{"name":"Institute for Cell Biology, University of Bonn 2 Department of Molecular Cell Biology , , 53121 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3932-4419","authenticated-orcid":false,"given":"Dirk G.","family":"de Rooij","sequence":"additional","affiliation":[{"name":"Utrecht University 3 Reproductive Biology Group, Division of Developmental Biology, Department of Biology, Faculty of Science , , 3584 CH Utrecht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9687-3730","authenticated-orcid":false,"given":"Carla","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Genetics, University of Bonn 4 Cell Migration Unit , , 53115 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Jokwitz","sequence":"additional","affiliation":[{"name":"Institute of Genetics, University of Bonn 4 Cell Migration Unit , , 53115 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5018-0060","authenticated-orcid":false,"given":"Walter","family":"Witke","sequence":"additional","affiliation":[{"name":"Institute of Genetics, University of Bonn 4 Cell Migration Unit , , 53115 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8272-0076","authenticated-orcid":false,"given":"Hubert","family":"Schorle","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn 1 Department of Developmental Pathology , , 53127 Bonn , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"2023102710033418600_DEV201593C1","article-title":"proDA: probabilistic dropout analysis for identifying differentially abundant proteins in label-free mass spectrometry","author":"Ahlmann-Eltze","year":"2021","journal-title":"bioRxiv"},{"key":"2023102710033418600_DEV201593C2","doi-asserted-by":"publisher","first-page":"e0175787","DOI":"10.1371\/journal.pone.0175787","article-title":"Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells","volume":"12","author":"Ahn","year":"2017","journal-title":"PLoS ONE"},{"key":"2023102710033418600_DEV201593C3","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3389\/fcell.2014.00056","article-title":"Role of the prion protein family in the gonads","volume":"2","author":"Allais-Bonnet","year":"2014","journal-title":"Front. 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