{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:27:39Z","timestamp":1790818059361,"version":"4.1.0"},"reference-count":77,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003768","name":"Universit\u00e9 de Strasbourg","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003768","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"crossref","award":["FRM FDT201904008001"],"award-info":[{"award-number":["FRM FDT201904008001"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-16-CE14-0017"],"award-info":[{"award-number":["ANR-16-CE14-0017"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-10-IDEX-0002-02"],"award-info":[{"award-number":["ANR-10-IDEX-0002-02"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-10-LABX-0030-INRT"],"award-info":[{"award-number":["ANR-10-LABX-0030-INRT"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>To investigate the role of the nuclear receptor NR5A1 in the testis after sex determination, we analyzed mice lacking NR5A1 in Sertoli cells (SCs) from embryonic day (E) 13.5 onwards. Ablation of Nr5a1 impaired the expression of genes characteristic of SC identity (e.g. Sox9 and Amh), caused SC death from E14.5 onwards through a Trp53-independent mechanism related to anoikis, and induced disorganization of the testis cords. Together, these effects caused germ cells to enter meiosis and die. Single-cell RNA-sequencing experiments revealed that NR5A1-deficient SCs changed their molecular identity: some acquired a \u2018pre-granulosa-like\u2019 cell identity, whereas other reverted to a \u2018supporting progenitor-like\u2019 cell identity, most of them being \u2018intersex\u2019 because they expressed both testicular and ovarian genes. Fetal Leydig cells (LCs) did not display significant changes, indicating that SCs are not required beyond E14.5 for their emergence or maintenance. In contrast, adult LCs were absent from postnatal testes. In addition, adult mutant males displayed persistence of M\u00fcllerian duct derivatives, decreased anogenital distance and reduced penis length, which could be explained by the loss of AMH and testosterone synthesis due to SC failure.<\/jats:p>","DOI":"10.1242\/dev.201710","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T15:43:57Z","timestamp":1702050237000},"source":"Crossref","is-referenced-by-count":8,"title":["Loss of NR5A1 in mouse Sertoli cells after sex determination changes cellular identity and induces cell death by anoikis"],"prefix":"10.1242","volume":"150","author":[{"given":"Sirine","family":"Souali-Crespo","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC) 1 , D\u00e9partement de G\u00e9n\u00e9tique Fonctionnelle et Cancer , , 1 rue Laurent Fries, BP-10142, F-67404 Illkirch Cedex , France"},{"name":"Centre National de la Recherche Scientifique (CNRS UMR7104), Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM U1258), Universit\u00e9 de Strasbourg (UNISTRA) 1 , D\u00e9partement de G\u00e9n\u00e9tique Fonctionnelle et Cancer , , 1 rue Laurent Fries, BP-10142, F-67404 Illkirch Cedex , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Condrea","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC) 1 , D\u00e9partement de G\u00e9n\u00e9tique Fonctionnelle et Cancer , , 1 rue Laurent Fries, BP-10142, F-67404 Illkirch Cedex , France"},{"name":"Centre National de la Recherche Scientifique (CNRS UMR7104), Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM U1258), Universit\u00e9 de Strasbourg (UNISTRA) 1 , D\u00e9partement de G\u00e9n\u00e9tique Fonctionnelle et Cancer , , 1 rue Laurent Fries, BP-10142, F-67404 Illkirch Cedex , 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