{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:26:56Z","timestamp":1791044816650,"version":"4.1.0"},"reference-count":46,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:00:00Z","timestamp":1695600000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100005623","name":"University of Tokushima","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005623","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused by competition between host germ cells and the transferred cells is a common complication and greatly reduces production efficiency. Here, we generated a gene-edited chicken, in which germ cells can be ablated in a drug-dependent manner, as recipients for gene-edited PGC transfer. We used the nitroreductase\/metronidazole (NTR\/Mtz) system for cell ablation, in which nitroreductase produces cytotoxic alkylating agents from administered metronidazole, causing cell apoptosis. The chicken Vasa homolog (CVH) gene locus was used to drive the expression of the nitroreductase gene in a germ cell-specific manner. In addition, a fluorescent protein gene, mCherry, was also placed in the CVH locus to visualize the PGCs. We named this system \u2018germ cell-specific autonomous removal induction\u2019 (gSAMURAI). gSAMURAI chickens will be an ideal recipient to produce offspring derived from transplanted exogenous germ cells.<\/jats:p>","DOI":"10.1242\/dev.202079","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T12:15:34Z","timestamp":1693829734000},"source":"Crossref","is-referenced-by-count":26,"title":["An inducible germ cell ablation chicken model for high-grade germline chimeras"],"prefix":"10.1242","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8969-9927","authenticated-orcid":false,"given":"Yi-Chen","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Research and Development, Setsuro Tech Inc. 1 , Tokushima 770-8503 , Japan"},{"name":"Laboratory for Embryology, Institute for Advanced Medical Sciences, Tokushima University 2 , Tokushima 770-8503 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5999-2505","authenticated-orcid":false,"given":"Daisuke","family":"Saito","sequence":"additional","affiliation":[{"name":"Faculty of Science, Kyushu University 3 Department of Biology , , Fukuoka 819-0395 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Graduate School of Science, Osaka Metropolitan University 4 Department of Biology , , Osaka 558-8585 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1860-0269","authenticated-orcid":false,"given":"Tatsuya","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Division of Research and Development, Setsuro Tech Inc. 1 , Tokushima 770-8503 , Japan"},{"name":"Laboratory for Embryology, Institute for Advanced Medical Sciences, Tokushima University 2 , Tokushima 770-8503 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,9,25]]},"reference":[{"key":"2023092512271681600_DEV202079C1","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1038\/s41467-020-20812-x","article-title":"Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating","volume":"12","author":"Ballantyne","year":"2021","journal-title":"Nat. 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Sci."},{"key":"2023092512271681600_DEV202079C6","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1080\/19420862.2017.1386825","article-title":"Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets","volume":"10","author":"Ching","year":"2018","journal-title":"MAbs"},{"key":"2023092512271681600_DEV202079C7","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1186\/s40104-022-00712-4","article-title":"Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens","volume":"13","author":"Choi","year":"2022","journal-title":"J. Anim. Sci. 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Biol."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.202079\/3118293\/dev202079.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.202079\/3165466\/dev202079.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.202079\/3165466\/dev202079.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T12:27:42Z","timestamp":1695644862000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/150\/18\/dev202079\/329056\/An-inducible-germ-cell-ablation-chicken-model-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,15]]},"references-count":46,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2023,9,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.202079","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,15]]},"article-number":"dev202079"}}