{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T15:35:50Z","timestamp":1784993750111,"version":"3.55.0"},"reference-count":39,"publisher":"The Company of Biologists","issue":"20","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["15H05864"],"award-info":[{"award-number":["15H05864"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["20A306"],"award-info":[{"award-number":["20A306"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["22J10710"],"award-info":[{"award-number":["22J10710"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Clarifying the mechanisms underlying shape alterations during insect metamorphosis is important for understanding exoskeletal morphogenesis. The large horn of the Japanese rhinoceros beetle Trypoxylus dichotomus is the result of drastic metamorphosis, wherein it appears as a rounded shape during pupation and then undergoes remodeling into an angular adult shape. However, the mechanical mechanisms underlying this remodeling process remain unknown. In this study, we investigated the remodeling mechanisms of the Japanese rhinoceros beetle horn by developing a physical simulation. We identified three factors contributing to remodeling by biological experiments \u2013 ventral adhesion, uneven shrinkage, and volume reduction \u2013 which were demonstrated to be crucial for transformation using a physical simulation. Furthermore, we corroborated our findings by applying the simulation to the mandibular remodeling of stag beetles. These results indicated that physical simulation applies to pupal remodeling in other beetles, and the morphogenic mechanism could explain various exoskeletal shapes.<\/jats:p>","DOI":"10.1242\/dev.202082","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T08:52:52Z","timestamp":1710319972000},"source":"Crossref","is-referenced-by-count":7,"title":["Adhesion and shrinkage transform the rounded pupal horn into an angular adult horn in Japanese rhinoceros beetle"],"prefix":"10.1242","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6961-9096","authenticated-orcid":false,"given":"Keisuke","family":"Matsuda","sequence":"first","affiliation":[{"name":"Graduate School of Frontier Biosciences, Osaka University 1 , Suita, Osaka 565-0871 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9420-5335","authenticated-orcid":false,"given":"Haruhiko","family":"Adachi","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Biosciences, Osaka University 1 , Suita, Osaka 565-0871 , Japan"},{"name":"Institute for Advanced Biosciences, Keio University 2 , Tsuruoka, Yamagata 997-0017 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0146-809X","authenticated-orcid":false,"given":"Hiroki","family":"Gotoh","sequence":"additional","affiliation":[{"name":"Faculty of Science, Shizuoka University 3 Department of Biological Sciences , , Shizuoka, Shizuoka 422-8529 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1968-8883","authenticated-orcid":false,"given":"Yasuhiro","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Micro Engineering, Graduate School of Engineering, Kyoto University 4 , Kyoto, Kyoto 616-8540 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0192-5526","authenticated-orcid":false,"given":"Shigeru","family":"Kondo","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Biosciences, Osaka University 1 , Suita, Osaka 565-0871 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"2024031312524931600_DEV202082C1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.mod.2018.06.003","article-title":"Anisotropy of cell division and epithelial sheet bending via apical constriction shape the complex folding pattern of beetle horn primordia","volume":"152","author":"Adachi","year":"2018","journal-title":"Mech. 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