{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:16:20Z","timestamp":1771035380322,"version":"3.50.1"},"reference-count":40,"publisher":"The Company of Biologists","issue":"5","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["GNT1063837"],"award-info":[{"award-number":["GNT1063837"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["DE140100500"],"award-info":[{"award-number":["DE140100500"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001215","name":"La Trobe University","doi-asserted-by":"crossref","award":["2000003053"],"award-info":[{"award-number":["2000003053"]}],"id":[{"id":"10.13039\/501100001215","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Australian Cranio-Maxillo-Facial Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Defective tissue fusion during mammalian embryogenesis results in congenital anomalies, such as exencephaly, spina bifida and cleft lip and\/or palate. The highly conserved transcription factor grainyhead-like 2 (Grhl2) is a crucial regulator of tissue fusion, with mouse models lacking GRHL2 function presenting with a fully penetrant open cranial neural tube, facial and abdominal clefting (abdominoschisis), and an open posterior neuropore. Here, we show that GRHL2 interacts with the soluble morphogen protein and bone morphogenetic protein (BMP) inhibitor noggin (NOG) to impact tissue fusion during development. The maxillary prominence epithelium in embryos lacking Grhl2 shows substantial morphological abnormalities and significant upregulation of NOG expression, together with aberrantly distributed pSMAD5-positive cells within the neural crest cell-derived maxillary prominence mesenchyme, indicative of disrupted BMP signalling. Reducing this elevated NOG expression (by generating Grhl2\u2212\/\u2212;Nog+\/\u2212 embryos) results in delayed embryonic lethality, partial tissue fusion rescue, and restoration of tissue form within the craniofacial epithelia. These data suggest that aberrant epithelial maintenance, partially regulated by noggin-mediated regulation of BMP-SMAD pathways, may underpin tissue fusion defects in Grhl2\u2212\/\u2212 mice.<\/jats:p>","DOI":"10.1242\/dev.202420","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T17:38:50Z","timestamp":1706809130000},"source":"Crossref","is-referenced-by-count":6,"title":["Grainyhead-like 2 interacts with noggin to regulate tissue fusion in mouse"],"prefix":"10.1242","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4755-8000","authenticated-orcid":false,"given":"Michael E.","family":"de Vries","sequence":"first","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"},{"name":"La Trobe University 2 Department of Physiology, Anatomy and Microbiology , , Melbourne, Victoria 3086 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina R.","family":"Carpinelli","sequence":"additional","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarrad N.","family":"Fuller","sequence":"additional","affiliation":[{"name":"La Trobe University 2 Department of Physiology, Anatomy and Microbiology , , Melbourne, Victoria 3086 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yindi","family":"Sutton","sequence":"additional","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren D.","family":"Partridge","sequence":"additional","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alana","family":"Auden","sequence":"additional","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Women and Children's Hospital 3 Australian Craniofacial Unit , , Adelaide, SA 5005 , Australia"},{"name":"University of Adelaide 4 Faculty of Health Sciences , , Adelaide, SA 5005 , Australia"},{"name":"School of Basic Medical Sciences, Nanjing Medical University 5 , Nanjing , 211166, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Jane","sequence":"additional","affiliation":[{"name":"Monash University Central Clinical School 1 Department of Medicine , , Prahran, Victoria 3004 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-9992","authenticated-orcid":false,"given":"Sebastian","family":"Dworkin","sequence":"additional","affiliation":[{"name":"La Trobe University 2 Department of Physiology, Anatomy and Microbiology , , Melbourne, Victoria 3086 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"2024022811512852000_DEV202420C1","doi-asserted-by":"publisher","first-page":"4647","DOI":"10.1242\/dev.129.19.4647","article-title":"Endogenous bone morphogenetic proteins regulate outgrowth and epithelial survival during avian lip fusion","volume":"129","author":"Ashique","year":"2002","journal-title":"Development"},{"key":"2024022811512852000_DEV202420C2","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/S1534-5807(02)00163-6","article-title":"Zebrafish DeltaNp63 is a direct target of Bmp signaling and encodes a transcriptional repressor blocking neural specification in the ventral ectoderm","volume":"2","author":"Bakkers","year":"2002","journal-title":"Dev. 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