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The excitatory neurons (e) of the medial (m) CN (eCNm) were recently divided into molecularly defined subdomains in the adult; however, how they are established during development is not known. We define molecular subdomains of the mouse embryonic eCNm using single-cell RNA-sequencing and spatial expression analysis, showing that they evolve during embryogenesis to prefigure the adult. Furthermore, eCNm are transcriptionally divergent from cells in the other nuclei by embryonic day 14.5. We previously showed that loss of the homeobox genes En1 and En2 leads to loss of approximately half of the embryonic eCNm. We demonstrate that mutation of En1\/2 in the embryonic eCNm results in death of specific posterior eCNm molecular subdomains and downregulation of TBR2 (EOMES) in an anterior embryonic subdomain, as well as reduced synaptic gene expression. We further reveal a similar function for EN1\/2 in mediating TBR2 expression, neuron differentiation and survival in the other excitatory neurons (granule and unipolar brush cells). Thus, our work defines embryonic eCNm molecular diversity and reveals conserved roles for EN1\/2 in the cerebellar excitatory neuron lineage.<\/jats:p>","DOI":"10.1242\/dev.202502","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T05:44:55Z","timestamp":1719207895000},"source":"Crossref","is-referenced-by-count":19,"title":["Engrailed transcription factors direct excitatory cerebellar neuron diversity and survival"],"prefix":"10.1242","volume":"151","author":[{"given":"Anjana","family":"Krishnamurthy","sequence":"first","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"},{"name":"Weill Cornell Graduate School of Medical Sciences 2 Neuroscience Program , , New York, NY 10065 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"},{"name":"Weill Cornell Graduate School of Medical Sciences 2 Neuroscience Program , , New York, NY 10065 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Sumru","family":"Bayin","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Stephen","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Nasef","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Lao","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7090-9605","authenticated-orcid":false,"given":"Alexandra L.","family":"Joyner","sequence":"additional","affiliation":[{"name":"Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center 1 Developmental Biology Program , , New York, NY 10021 , USA"},{"name":"Weill Cornell Graduate School of Medical Sciences 2 Neuroscience Program , , New York, NY 10065 , USA"},{"name":"Weill Cornell Graduate School of Medical Sciences 3 Biochemistry, Cell and Molecular Biology Program , , New York, NY 10065 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,7,26]]},"reference":[{"key":"2024072908055117400_DEV202502C1","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.ydbio.2015.10.008","article-title":"En1 directs superior olivary complex neuron positioning, survival, and expression of FoxP1","volume":"408","author":"Altieri","year":"2015","journal-title":"Dev. 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