{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:20:59Z","timestamp":1788884459226,"version":"build-2803163510"},"reference-count":24,"publisher":"The Company of Biologists","issue":"11","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 GM138705"],"award-info":[{"award-number":["R01 GM138705"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Germline maintenance relies on adult stem cells to continually replenish lost gametes over a lifetime and respond to external cues altering the demands on the tissue. Mating worsens germline homeostasis over time, yet a negative impact on stem cell behavior has not been explored. Using extended live imaging of the Drosophila testis stem cell niche, we find that short periods of mating in young males disrupts cytokinesis in germline stem cells (GSCs). This defect leads to failure of abscission, preventing release of differentiating cells from the niche. We find that GSC abscission failure is caused by increased Ecdysone hormone signaling induced upon mating, which leads to disrupted somatic encystment of the germline. Abscission failure is rescued by isolating males from females, but recurs with resumption of mating. Importantly, reiterative mating also leads to increased GSC loss, requiring increased restoration of stem cells via symmetric renewal and de-differentiation. Together, these results suggest a model whereby acute mating results in hormonal changes that negatively impact GSC cytokinesis but preserves the stem cell population.<\/jats:p>","DOI":"10.1242\/dev.202542","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T12:52:38Z","timestamp":1717505558000},"source":"Crossref","is-referenced-by-count":12,"title":["Mating-induced Ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis"],"prefix":"10.1242","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3098-3314","authenticated-orcid":false,"given":"Tiffany V.","family":"Roach","sequence":"first","affiliation":[{"name":"Drexel University Department of Biology , , Chestnut St, Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4575-5514","authenticated-orcid":false,"given":"Kari F.","family":"Lenhart","sequence":"additional","affiliation":[{"name":"Drexel University Department of Biology , , Chestnut St, Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"key":"2024060413562745100_DEV202542C1","doi-asserted-by":"publisher","first-page":"e1006123","DOI":"10.1371\/journal.pgen.1006123","article-title":"Mating-induced increase in germline stem cells via the neuroendocrine system in female Drosophila","volume":"12","author":"Ameku","year":"2016","journal-title":"PLoS Genet."},{"key":"2024060413562745100_DEV202542C2","doi-asserted-by":"publisher","first-page":"e1008062","DOI":"10.1371\/journal.pgen.1008062","article-title":"Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment","volume":"15","author":"Chang","year":"2019","journal-title":"PLoS Genet."},{"key":"2024060413562745100_DEV202542C3","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.ydbio.2013.12.013","article-title":"Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila","volume":"386","author":"Domanitskaya","year":"2014","journal-title":"Dev. 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