{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T23:04:38Z","timestamp":1762643078492,"version":"3.41.2"},"reference-count":64,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T00:00:00Z","timestamp":1718409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP23jf0126001","JP23gm1110008"],"award-info":[{"award-number":["JP23jf0126001","JP23gm1110008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["23K20043","23H00399","23H03036","22K19429","23K27727"],"award-info":[{"award-number":["23K20043","23H00399","23H03036","22K19429","23K27727"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Spermatogonial stem cells (SSCs) undergo self-renewal division to sustain spermatogenesis. Although it is possible to derive SSC cultures in most mouse strains, SSCs from a 129 background never proliferate under the same culture conditions, suggesting they have distinct self-renewal requirements. Here, we established long-term culture conditions for SSCs from mice of the 129 background (129 mice). An analysis of 129 testes showed significant reduction of GDNF and CXCL12, whereas FGF2, INHBA and INHBB were higher than in testes of C57BL\/6 mice. An analysis of undifferentiated spermatogonia in 129 mice showed higher expression of Chrna4, which encodes an acetylcholine (Ach) receptor component. By supplementing medium with INHBA and Ach, SSC cultures were derived from 129 mice. Following lentivirus transduction for marking donor cells, transplanted cells re-initiated spermatogenesis in infertile mouse testes and produced transgenic offspring. These results suggest that the requirements of SSC self-renewal in mice are diverse, which has important implications for understanding self-renewal mechanisms in various animal species.<\/jats:p>","DOI":"10.1242\/dev.202553","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T10:25:29Z","timestamp":1719483929000},"source":"Crossref","is-referenced-by-count":1,"title":["Spermatogonial stem cells in the 129 inbred strain exhibit unique requirements for self-renewal"],"prefix":"10.1242","volume":"151","author":[{"given":"Mito","family":"Kanatsu-Shinohara","sequence":"first","affiliation":[{"name":"Graduate School of Medicine, Kyoto University 1 Department of Molecular Genetics , , Kyoto 606-8501 , Japan"},{"name":"AMED-CREST, AMED 1-7-1 Otemachi 2 , Chiyodaku, Tokyo 100-0004 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0022-3947","authenticated-orcid":false,"given":"Takuya","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Center for iPS Cell Research and Application, Kyoto University 3 Department of Life Science Frontiers , , Kyoto 606-8507 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Kyoto University 1 Department of Molecular Genetics , , Kyoto 606-8501 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianjiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Kyoto University 1 Department of Molecular Genetics , , Kyoto 606-8501 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9613-7022","authenticated-orcid":false,"given":"Takashi","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Kyoto University 1 Department of Molecular Genetics , , Kyoto 606-8501 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"2024062710252233000_DEV202553C1","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1530\/REP-08-0140","article-title":"Inhibin, activin, follistatin and FSH serum levels and testicular production are highly modulated during the first spermatogenic wave in mice","volume":"136","author":"Barakat","year":"2008","journal-title":"Reproduction"},{"key":"2024062710252233000_DEV202553C2","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1093\/molehr\/gah245","article-title":"Acetylcholine causes an increase of intracellular calcium in human sperm","volume":"11","author":"Bray","year":"2006","journal-title":"Mol. 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