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We used a Cre driver to delete Ebf1 at the otocyst stage before development of the cochlea. We examined the cochlea at postnatal day (P) 1 and found that the sensory epithelium had doubled in size but the length of the cochlear duct was unaffected. We also found that deletion of Ebf1 led to ectopic sensory patches in the K\u00f6lliker's organ. Innervation of the developing organ of Corti was disrupted with no obvious spiral bundles. The ectopic patches were also innervated. All the extra hair cells (HCs) within the sensory epithelium and K\u00f6lliker's organ contained mechanoelectrical transduction channels, as indicated by rapid uptake of FM1-43. The excessive numbers of HCs were still present in the adult Ebf1 conditional knockout (cKO) animal. The animals had significantly elevated auditory brainstem response thresholds, suggesting that this gene is essential for hearing development.<\/jats:p>","DOI":"10.1242\/dev.202816","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T11:15:54Z","timestamp":1721646954000},"source":"Crossref","is-referenced-by-count":6,"title":["Deletion of the <i>Ebf1<\/i>, a mouse deafness gene, causes a dramatic increase in hair cells and support cells of the organ of Corti"],"prefix":"10.1242","volume":"151","author":[{"given":"Kathryn G.","family":"Powers","sequence":"first","affiliation":[{"name":"University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA"},{"name":"University of Washington 2 Program in Molecular and Cellular Biology , , Seattle, WA 98195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3532-3075","authenticated-orcid":false,"given":"Brent A.","family":"Wilkerson","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina 3 Department of Otolaryngology-Head and Neck Surgery , , Charleston, SC 29425 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kylie E.","family":"Beach","sequence":"additional","affiliation":[{"name":"University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie S.","family":"Seo","sequence":"additional","affiliation":[{"name":"University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose S.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashton N.","family":"Baxter","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina 3 Department of Otolaryngology-Head and Neck Surgery , , Charleston, SC 29425 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Medical University of South Carolina 3 Department of Otolaryngology-Head and Neck Surgery , , Charleston, SC 29425 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2559-4218","authenticated-orcid":false,"given":"Olivia","family":"Bermingham-McDonogh","sequence":"additional","affiliation":[{"name":"University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"2024082010312160900_DEV202816C1","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.stem.2011.09.001","article-title":"Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice","volume":"9","author":"Arnold","year":"2011","journal-title":"Cell Stem Cell"},{"key":"2024082010312160900_DEV202816C2","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1172\/JCI97248","article-title":"Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea","volume":"128","author":"Atkinson","year":"2018","journal-title":"J. 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