{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:36:42Z","timestamp":1790818602688,"version":"4.1.0"},"reference-count":69,"publisher":"The Company of Biologists","issue":"17","license":[{"start":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["211944\/Z\/18\/Z"],"award-info":[{"award-number":["211944\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001703","name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001703","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Although the advent of organoids has opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here, we established organoids from the thymus, the lymphoid organ responsible for T-cell development. We identified conditions enabling mouse thymic epithelial progenitor cell proliferation and development into organoids with diverse cell populations and transcriptional profiles resembling in vivo thymic epithelial cells (TECs) more closely than traditional TEC cultures. In contrast to these two-dimensional cultures, thymic epithelial organoids maintained thymus functionality in vitro and mediated physiological T-cell development upon reaggregation with T-cell progenitors. The reaggregates showed in vivo-like epithelial diversity and the ability to attract T-cell progenitors. Thymic epithelial organoids are the first organoids originating from the stromal compartment of a lymphoid organ. They provide new opportunities to study TEC biology and T-cell development in vitro, paving the way for future thymic regeneration strategies in ageing or acute injuries.<\/jats:p>","DOI":"10.1242\/dev.202853","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T07:00:18Z","timestamp":1721631618000},"source":"Crossref","is-referenced-by-count":25,"title":["Thymic epithelial organoids mediate T-cell development"],"prefix":"10.1242","volume":"151","author":[{"given":"Tania","family":"H\u00fcbscher","sequence":"first","affiliation":[{"name":"Institute of Bioengineering 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"School of Life Sciences and School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Francisco","family":"Lorenzo-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"School of Life Sciences and School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Barthlott","sequence":"additional","affiliation":[{"name":"University of Basel 2 Pediatric Immunology, Department of Biomedicine , , 4058 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Tillard","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"School of Life Sciences and School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob J.","family":"Langer","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"School of Life Sciences and School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Rouse","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine, Institute for Regeneration and Repair, School of Biological Sciences, University of Edinburgh 3 , Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Clare","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Centre for Regenerative Medicine, Institute for Regeneration and Repair, School of Biological Sciences, University of Edinburgh 3 , Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Holl\u00e4nder","sequence":"additional","affiliation":[{"name":"University of Basel 2 Pediatric Immunology, Department of Biomedicine , , 4058 Basel , Switzerland"},{"name":"University of Oxford 4 Department of Paediatrics , , Oxford, OX3 9DU , UK"},{"name":"University of Oxford 5 Institute of Developmental and Regenerative Medicine , , Oxford, OX3 7TY , UK"},{"name":"Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETHZ) 6 Department of Biosystems Science and Engineering , , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5898-305X","authenticated-orcid":false,"given":"Matthias P.","family":"Lutolf","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"School of Life Sciences and School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) 1 Laboratory of Stem Cell Bioengineering , , , 1015 Lausanne , Switzerland"},{"name":"Institute of Human Biology (IHB), Pharma Research and Early Development, Roche Innovation Center Basel 7 , 4058 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,9,3]]},"reference":[{"key":"2024090310232088300_DEV202853C1","doi-asserted-by":"publisher","first-page":"858","DOI":"10.3389\/fimmu.2020.00858","article-title":"Generation and regeneration of thymic epithelial cells","volume":"11","author":"Alawam","year":"2020","journal-title":"Front. 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