{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T21:21:54Z","timestamp":1787952114132,"version":"build-2784847793"},"reference-count":68,"publisher":"The Company of Biologists","issue":"22","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32202925"],"award-info":[{"award-number":["32202925"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31972785"],"award-info":[{"award-number":["31972785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"crossref","award":["LQ23C19001"],"award-info":[{"award-number":["LQ23C19001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"crossref","award":["LR21C190001"],"award-info":[{"award-number":["LR21C190001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Seasonal spermatogenesis in fish is driven by spermatogonial stem cells (SSCs), which undergo a complex cellular process to differentiate into mature sperm. In this study, we characterized spermatogenesis in the large yellow croaker (Larimichthys crocea), a marine fish of significant commercial value, based on a high-resolution single-cell RNA-sequencing atlas of testicular cells from three distinct developmental stages: juvenile, adult differentiating and regressed testes. We detailed a continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, elucidating the molecular events involved in spermatogenesis. We uncovered dynamic heterogeneity in cellular compositions throughout the annual reproductive cycle, accompanied by strong molecular signatures within specific testicular cells. Notably, we identified a distinct population of SSCs and observed a critical metabolic transition from glycolysis to oxidative phosphorylation, enhancing our understanding of the biochemical and molecular characteristics of SSCs. Additionally, we elucidated the interactions between somatic cells and spermatogonia, illuminating the mechanisms that regulate SSC development. Overall, this work enhances our understanding of spermatogenesis in seasonal breeding teleosts and provides essential insights for the further conservation and culture of SSCs.<\/jats:p>","DOI":"10.1242\/dev.203142","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T11:23:31Z","timestamp":1732101811000},"source":"Crossref","is-referenced-by-count":19,"title":["Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost"],"prefix":"10.1242","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5038-5872","authenticated-orcid":false,"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Zhejiang Marine Fisheries Research Institute 1 Key Laboratory of Mariculture and Enhancement of Zhejiang Province , , Zhoushan 316021 ,","place":["China"]},{"name":"Ocean and Fisheries Research Institute, Zhejiang Ocean University 2 , Zhoushan 316021 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Zhejiang Marine Fisheries Research Institute 1 Key Laboratory of Mariculture and Enhancement of Zhejiang Province , , Zhoushan 316021 ,","place":["China"]},{"name":"School of Fisheries, Zhejiang Ocean University 3 , Zhoushan 316022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1210-9279","authenticated-orcid":false,"given":"Gary","family":"Wessel","sequence":"additional","affiliation":[{"name":"Brown University 4 Department of Molecular and Cell Biology and Biochemistry , , Providence, RI 02912 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Hu","sequence":"additional","affiliation":[{"name":"Zhejiang Marine Fisheries Research Institute 1 Key Laboratory of Mariculture and Enhancement of Zhejiang Province , , Zhoushan 316021 ,","place":["China"]},{"name":"Ocean and Fisheries Research Institute, Zhejiang Ocean University 2 , Zhoushan 316021 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6935-9375","authenticated-orcid":false,"given":"Dongdong","family":"Xu","sequence":"additional","affiliation":[{"name":"Zhejiang Marine Fisheries Research Institute 1 Key Laboratory of Mariculture and Enhancement of Zhejiang Province , , Zhoushan 316021 ,","place":["China"]},{"name":"Ocean and Fisheries Research Institute, Zhejiang Ocean University 2 , Zhoushan 316021 ,","place":["China"]},{"name":"Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University 5 , Ningbo 315100 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,11,20]]},"reference":[{"key":"2024112016224739000_DEV203142C1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.2164\/jandrol.109.008318","article-title":"Fetal Leydig cells: progenitor cell maintenance and differentiation","volume":"31","author":"Barsoum","year":"2010","journal-title":"J. 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