{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T19:25:12Z","timestamp":1778009112699,"version":"3.51.4"},"reference-count":72,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["204832\/Z\/16\/Z"],"award-info":[{"award-number":["204832\/Z\/16\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["227415\/Z\/23\/Z"],"award-info":[{"award-number":["227415\/Z\/23\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000770","name":"University of Manchester","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000770","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Live imaging of transcription in the Drosophila embryo using the MS2 or PP7 systems is transforming our understanding of transcriptional regulation. However, insertion of MS2\/PP7 stem-loops into endogenous genes requires laborious CRISPR genome editing. Here, we exploit the previously described Minos-mediated integration cassette (MiMIC) transposon system in Drosophila to establish a method for simply and rapidly inserting MS2\/PP7 cassettes into any of the thousands of genes carrying a MiMIC insertion. In addition to generating a variety of stem-loop donor fly stocks, we have made new stocks expressing the complementary coat proteins fused to different fluorescent proteins. We show the utility of this MiMIC-based approach by MS2\/PP7 tagging of endogenous genes and the long non-coding RNA roX1, then imaging their transcription in living embryos. We also present live transcription data from larval brains, the wing disc and ovary, thereby extending the tissues that can be studied using the MS2\/PP7 system. Overall, this first high-throughput method for tagging mRNAs in Drosophila will facilitate the study of transcription dynamics of thousands of endogenous genes in a range of Drosophila tissues.<\/jats:p>","DOI":"10.1242\/dev.204294","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T10:58:23Z","timestamp":1732532303000},"source":"Crossref","is-referenced-by-count":2,"title":["A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0982-3700","authenticated-orcid":false,"given":"Lauren Forbes","family":"Beadle","sequence":"first","affiliation":[{"name":"The University of Manchester Faculty of Biology, Medicine and Health , , Manchester M13 9PT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-0278","authenticated-orcid":false,"given":"Catherine","family":"Sutcliffe","sequence":"additional","affiliation":[{"name":"The University of Manchester Faculty of Biology, Medicine and Health , , Manchester M13 9PT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8379-7830","authenticated-orcid":false,"given":"Hilary L.","family":"Ashe","sequence":"additional","affiliation":[{"name":"The University of Manchester Faculty of Biology, Medicine and Health , , Manchester M13 9PT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"2024121613365337800_DEV204294C1","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1534\/g3.113.008508","article-title":"Generation of a useful roX1 allele by targeted gene conversion","volume":"4","author":"Apte","year":"2014","journal-title":"G3 (Bethesda)"},{"key":"2024121613365337800_DEV204294C2","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.mod.2015.08.004","article-title":"Following the intracellular localization of the iab-8ncRNA of the bithorax complex using the MS2-MCP-GFP system","volume":"138","author":"Arib","year":"2015","journal-title":"Mech. 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