{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:01:16Z","timestamp":1790913676901,"version":"4.1.0"},"reference-count":59,"publisher":"The Company of Biologists","issue":"22","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R35GM142950"],"award-info":[{"award-number":["R35GM142950"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["T32GM141848"],"award-info":[{"award-number":["T32GM141848"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["T32HD007491"],"award-info":[{"award-number":["T32HD007491"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007747","name":"University of Utah","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007747","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Utah Alumni Association"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Vertebrate spermatogonial stem cells maintain sperm production over the lifetime of an animal, but fertility declines with age. Although morphological studies have informed our understanding of typical spermatogenesis, the molecular and cellular mechanisms underlying the maintenance and decline of spermatogenesis are not yet understood. We used single-cell RNA sequencing to generate a developmental atlas of the aging zebrafish testis. All testes contained spermatogonia, but we observed a progressive decline in spermatogenesis that correlated with age. Testes from some older males only contained spermatogonia and a reduced population of spermatocytes. Spermatogonia in older males were transcriptionally distinct from spermatogonia in testes capable of robust spermatogenesis. Immune cells including macrophages and lymphocytes drastically increased in abundance in testes that could not complete spermatogenesis. Our developmental atlas reveals the cellular changes as the testis ages and defines a molecular roadmap for the regulation of spermatogenesis.<\/jats:p>","DOI":"10.1242\/dev.204319","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T08:31:09Z","timestamp":1730190669000},"source":"Crossref","is-referenced-by-count":26,"title":["Germ cell progression through zebrafish spermatogenesis declines with age"],"prefix":"10.1242","volume":"151","author":[{"given":"Andrea L.","family":"Sposato","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey L.","family":"Hollins","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren R.","family":"Llewellyn","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna M.","family":"Weber","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison N.","family":"Schrock","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Farrell","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH 2 Division of Developmental Biology , , Bethesda, MD 20814 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3978-6058","authenticated-orcid":false,"given":"James A.","family":"Gagnon","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah 1 , Salt Lake City, UT 84112 ,","place":["USA"]},{"name":"Henry Eyring Center for Cell and Genome Science, University of Utah 3 , Salt Lake City, UT 84112 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"2024111911002471900_DEV204319C1","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/S0140-6736(20)32667-2","article-title":"Male infertility","volume":"397","author":"Agarwal","year":"2021","journal-title":"The Lancet"},{"key":"2024111911002471900_DEV204319C2","doi-asserted-by":"publisher","first-page":"583304","DOI":"10.3389\/fimmu.2020.583304","article-title":"Immune cell subtypes and their function in the testis","volume":"11","author":"Bhushan","year":"2020","journal-title":"Front. 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