{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:17:15Z","timestamp":1790381835894,"version":"4.1.0"},"reference-count":49,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35 GM136270"],"award-info":[{"award-number":["R35 GM136270"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["T32GM008216"],"award-info":[{"award-number":["T32GM008216"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006920","name":"University of Pennsylvania","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006920","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Stem cells are essential to repair and regenerate tissues, and often reside in a niche that controls their behavior. Here, we use the Drosophila testis niche, a paradigm for niche\u2013stem cell interactions, to address the cell biological features that maintain niche structure and function during its steady-state operation. We report enrichment of Myosin II (MyoII) and a key regulator of actomyosin contractility (AMC), Rho Kinase (ROK), within the niche cell cortex at the interface with germline stem cells (GSCs). Compromising MyoII and ROK disrupts niche architecture, suggesting that AMC in niche cells is important to maintain its reproducible structure. Furthermore, defects in niche architecture disrupt GSC function. Our data suggest that the niche signals less robustly to adjacent germ cells yet permits increased numbers of cells to respond to the signal. Finally, compromising MyoII in niche cells leads to increased misorientation of centrosomes in GSCs as well as defects in the centrosome orientation checkpoint. Ultimately, this work identifies a crucial role for AMC-dependent maintenance of niche structure to ensure a proper complement of stem cells that correctly execute divisions.<\/jats:p>","DOI":"10.1242\/dev.204498","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T10:20:53Z","timestamp":1733134853000},"source":"Crossref","is-referenced-by-count":5,"title":["Maintenance of niche architecture requires actomyosin and enables proper stem cell signaling and oriented division in the\n                    <i>Drosophila<\/i>\n                    testis"],"prefix":"10.1242","volume":"152","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3876-8811","authenticated-orcid":false,"given":"Gabriela S.","family":"Vida","sequence":"first","affiliation":[{"name":"Perelman School of Medicine at the University of Pennsylvania 1 Department of Cell and Developmental Biology , , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]},{"name":"The Penn Institute for Regenerative Medicine 2 , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-1965","authenticated-orcid":false,"given":"Elizabeth","family":"Botto","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine at the University of Pennsylvania 1 Department of Cell and Developmental Biology , , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]},{"name":"The Penn Institute for Regenerative Medicine 2 , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4131-5511","authenticated-orcid":false,"given":"Stephen","family":"DiNardo","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine at the University of Pennsylvania 1 Department of Cell and Developmental Biology , , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]},{"name":"The Penn Institute for Regenerative Medicine 2 , 421 Curie Blvd, Philadelphia, PA 19104 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,1,9]]},"reference":[{"key":"2025011509260257900_DEV204498C1","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1242\/dev.086413","article-title":"Hedgehog is required for CySC self-renewal but does not contribute to the GSC niche in the Drosophila testis","volume":"140","author":"Amoyel","year":"2013","journal-title":"Development"},{"key":"2025011509260257900_DEV204498C2","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1016\/j.devcel.2022.03.009","article-title":"Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche","volume":"57","author":"Anllo","year":"2022","journal-title":"Dev. 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