{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:57:11Z","timestamp":1783605431613,"version":"3.55.0"},"reference-count":59,"publisher":"The Company of Biologists","issue":"22","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-2\/"}],"funder":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R01-HD086175"],"award-info":[{"award-number":["R01-HD086175"]}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R01-HD097206"],"award-info":[{"award-number":["R01-HD097206"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Excessive mature follicle accumulation in ovaries harms oocyte health and offspring viability. The mechanism by which females count mature follicles in their ovaries remains a mystery. Recent work demonstrated that Drosophila insulin-like peptide 8 (ILP8) is expressed in mature follicle cells but not in younger follicles. Here, we found that ILP8 is not essential for mating-induced ovulation but plays a crucial role in inducing ovulation in virgin females and preventing excessive accumulation of mature follicles. Excessive accumulation of mature follicles in ilp8-knockdown females leads to poor oocyte quality. In addition, knockdown of Lgr3 (Leucine-rich repeat-containing G protein-coupled receptor 3), encoding a previously identified ILP8 receptor, globally or neuronally showed similar ovulation\/egg laying defects, accumulation of mature follicles and poor oocyte quality in virgin females. Therefore, ILP8 functions as a mature follicle sensor to prevent excessive accumulation of mature follicles and maintain oocyte quality, likely through the neuronal Lgr3 receptor in virgin females. Our findings suggest that ILP8\/Lgr3 is likely essential for maintaining the optimal reproductive fitness of virgin females and for species survival in the wild, where mating success is not guaranteed.<\/jats:p>","DOI":"10.1242\/dev.204827","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:37:14Z","timestamp":1761899834000},"source":"Crossref","is-referenced-by-count":5,"title":["ILP8 serves as a mature follicle sensor to prevent excessive accumulation of mature follicles in\n                    <i>Drosophila<\/i>\n                    ovaries and oocyte aging"],"prefix":"10.1242","volume":"152","author":[{"given":"Rebecca","family":"Oramas","sequence":"first","affiliation":[{"name":"University of Connecticut 1 Department of Physiology & Neurobiology , , Storrs, CT 06269 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Yacuk","sequence":"additional","affiliation":[{"name":"University of Connecticut 1 Department of Physiology & Neurobiology , , Storrs, CT 06269 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella E.","family":"Cho","sequence":"additional","affiliation":[{"name":"University of Connecticut 1 Department of Physiology & Neurobiology , , Storrs, CT 06269 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie R.","family":"Aloisio","sequence":"additional","affiliation":[{"name":"University of Connecticut 1 Department of Physiology & Neurobiology , , Storrs, CT 06269 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6015-738X","authenticated-orcid":false,"given":"Jianjun","family":"Sun","sequence":"additional","affiliation":[{"name":"University of Connecticut 1 Department of Physiology & Neurobiology , , Storrs, CT 06269 ,","place":["USA"]},{"name":"Institute for Systems Genomics, University of Connecticut 2 , Storrs, CT 06269 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"2025112404522487100_DEV204827C1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1146\/annurev-ento-120709-144823","article-title":"Insect Seminal Fluid Proteins: Identification and Function","volume":"56","author":"Avila","year":"2011","journal-title":"Annu. 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