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Niche cell identity must be properly specified during development to form a tissue capable of functioning in the adult. Here, we show that the Tbx1 ortholog org-1 is expressed in Drosophila testis niche cells in response to Slit and FGF signals. org-1 is expressed during niche development and is required to specify niche cell identity. org-1 mutants specified fewer niche cells, and those cells showed disruption of niche-specific markers, including loss of the niche adhesion protein Fas3 and reduced hedgehog expression. We found that org-1 expression in somatic gonadal precursors is capable of inducing formation of additional niche cells. Disrupted niche identity in org-1 mutants caused defects in niche assembly and functionality. We found that the conserved transcription factor islet is expressed in response to org-1 and show that islet functions downstream to mediate niche identity and assembly. This work identifies a previously unreported role for org-1 in niche establishment.<\/jats:p>","DOI":"10.1242\/dev.204914","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T11:07:16Z","timestamp":1765451236000},"source":"Crossref","is-referenced-by-count":2,"title":["The Tbx1 ortholog\n                    <i>org-1<\/i>\n                    is required to establish testis stem cell niche identity in\n                    <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"153","author":[{"given":"Patrick","family":"Hofe","sequence":"first","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3803-8218","authenticated-orcid":false,"given":"Ariel","family":"Harrington","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tynan","family":"Gardner","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine at the University of Pennsylvania 2 , Philadelphia, PA 19104 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4131-5511","authenticated-orcid":false,"given":"Stephen","family":"DiNardo","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine at the University of Pennsylvania 2 , Philadelphia, PA 19104 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5482-5882","authenticated-orcid":false,"given":"Lauren","family":"Anllo","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"2026022610120329900_DEV204914C1","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1242\/dev.086413","article-title":"Hedgehog is required for CySC self-renewal but does not contribute to the GSC niche in the Drosophila testis","volume":"140","author":"Amoyel","year":"2013","journal-title":"Development"},{"key":"2026022610120329900_DEV204914C2","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1016\/j.devcel.2022.03.009","article-title":"Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche","volume":"57","author":"Anllo","year":"2022","journal-title":"Dev. 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