{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:20:47Z","timestamp":1788261647574,"version":"build-2803163510"},"reference-count":106,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006352","name":"Virginia and D.K. Ludwig Fund for Cancer Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006352","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005492","name":"Stanford University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005492","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The capacity to detect and respond to injury is crucial for the recovery and long-term survival of many organisms. Wnts are commonly induced by tissue damage but how they become activated transcriptionally is not well understood. Here, we report that mouse Wnt1 and Wnt10b are induced following injury in both lung and muscle. These Wnts occupy the same chromosome and are transcribed in opposite directions with 12\u2005kb between them. We identified a highly conserved cis-acting regulatory region (enhancer) residing between Wnt1 and Wnt10b that, when fused to a lacZ reporter, is activated post-injury. This enhancer harbors putative AP-1-binding sites that are required for reporter activity, a feature observed in other injury-responsive enhancers. Injured muscles in mice carrying a germline deletion of the enhancer region display reduced Wnt1 and Wnt10b expression and show elevated intramuscular adipogenesis, which can be a hallmark of impaired muscle regeneration or tissue maintenance. Enhancer redundancy is common in development, but our in vivo analysis shows that loss of a single injury-responsive regulatory region in adult tissues can produce a detectable phenotype.<\/jats:p>","DOI":"10.1242\/dev.204933","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T09:47:41Z","timestamp":1763632061000},"source":"Crossref","is-referenced-by-count":3,"title":["Deletion of an enhancer that controls Wnt gene expression following tissue injury produces increased adipogenesis in regenerated muscle"],"prefix":"10.1242","volume":"152","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1701-3989","authenticated-orcid":false,"given":"Catriona Y.","family":"Logan","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4725-5161","authenticated-orcid":false,"given":"Xinhong","family":"Lim","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8831-0892","authenticated-orcid":false,"given":"Matt P.","family":"Fish","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2598-8808","authenticated-orcid":false,"given":"Makiko","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8585-3386","authenticated-orcid":false,"given":"Brooke","family":"Swain","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7082-3748","authenticated-orcid":false,"given":"Roel","family":"Nusse","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]},{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine , Department of Developmental Biology , , Stanford, CA 94305,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"key":"2026090107091085900_DEV204933C1","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.ajpath.2021.10.003","article-title":"Molecular characterization of skeletal muscle dysfunction in sigma 1 receptor (Sigmar1) knockout mice","volume":"192","author":"Aishwarya","year":"2022","journal-title":"Am. J. Pathol."},{"key":"2026090107091085900_DEV204933C2","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1111\/wrr.12148","article-title":"A statistical analysis of murine incisional and excisional acute wound models","volume":"22","author":"Ansell","year":"2014","journal-title":"Wound Repair. Regen."},{"key":"2026090107091085900_DEV204933C3","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1038\/s42003-021-02118-w","article-title":"Wnt signaling in lung development, regeneration, and disease progression","volume":"4","author":"Aros","year":"2021","journal-title":"Commun. Biol."},{"key":"2026090107091085900_DEV204933C4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13395-016-0118-2","article-title":"A novel method for the quantification of fatty infiltration in skeletal muscle","volume":"7","author":"Biltz","year":"2017","journal-title":"Skelet. Muscle"},{"key":"2026090107091085900_DEV204933C5","first-page":"607","article-title":"Chromosomal insertion of foreign DNA","volume":"36","author":"Bishop","year":"1996","journal-title":"Reprod. Nutr. Dev."},{"key":"2026090107091085900_DEV204933C6","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/S0022-2275(20)41129-0","article-title":"Perilipin is located on the surface layer of intracellular lipid droplets in adipocyte","volume":"36","author":"Blanchette-Mackie","year":"1995","journal-title":"J. Lipid Res."},{"key":"2026090107091085900_DEV204933C7","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.stem.2007.10.006","article-title":"A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis","volume":"2","author":"Brack","year":"2008","journal-title":"Cell Stem Cell"},{"key":"2026090107091085900_DEV204933C8","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1242\/dev.128.8.1253","article-title":"Inactivation of the \u03b2-catenin gene by Wnt1-Cre-mediated deletion results in dramatic brain malformation and failure of craniofacial development","volume":"128","author":"Brault","year":"2001","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C9","doi-asserted-by":"publisher","DOI":"10.1101\/2025.03.18.643955","article-title":"Wnt signaling restores evolutionary loss of regenerative potential in Hydra","author":"Campos","year":"2025","journal-title":"bioRxiv"},{"key":"2026090107091085900_DEV204933C10","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1002\/dvg.20651","article-title":"Generation of mice with a conditional null allele for Wntless","volume":"48","author":"Carpenter","year":"2010","journal-title":"Genesis"},{"key":"2026090107091085900_DEV204933C11","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.bone.2011.08.010","article-title":"Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a \u03b2-catenin-dependent mechanism","volume":"50","author":"Cawthorn","year":"2012","journal-title":"Bone"},{"key":"2026090107091085900_DEV204933C12","doi-asserted-by":"publisher","first-page":"e60562","DOI":"10.7554\/eLife.60562","article-title":"Generic injuries are sufficient to induce ectopic Wnt organizers in Hydra","volume":"10","author":"Cazet","year":"2021","journal-title":"eLife"},{"key":"2026090107091085900_DEV204933C13","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1007\/s00125-006-0144-4","article-title":"WNT10B mutations in human obesity","volume":"49","author":"Christodoulides","year":"2006","journal-title":"Diabetologia"},{"key":"2026090107091085900_DEV204933C14","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.tem.2008.09.002","article-title":"Adipogenesis and WNT signalling","volume":"20","author":"Christodoulides","year":"2009","journal-title":"Trends Endocrinol. Metab."},{"key":"2026090107091085900_DEV204933C15","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1006\/dbio.1997.8762","article-title":"A 5.5-kb enhancer is both necessary and sufficient for regulation of Wnt-1 transcription in vivo","volume":"192","author":"Danielian","year":"1997","journal-title":"Dev. Biol."},{"key":"2026090107091085900_DEV204933C16","doi-asserted-by":"publisher","first-page":"104954","DOI":"10.1016\/j.isci.2022.104954","article-title":"Global chromatin accessibility profiling analysis reveals a chronic activation state in aged muscle stem cells","volume":"25","author":"Dong","year":"2022","journal-title":"iScience"},{"key":"2026090107091085900_DEV204933C17","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.1242\/dev.120.8.2213","article-title":"Cis-acting regulatory sequences governing Wnt-1 expression in the developing mouse CNS","volume":"120","author":"Echelard","year":"1994","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C18","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1038\/s41588-023-01526-4","article-title":"Reactivation of embryonic genetic programs in tissue regeneration and disease","volume":"55","author":"Fazilaty","year":"2023","journal-title":"Nat. Genet."},{"key":"2026090107091085900_DEV204933C19","doi-asserted-by":"publisher","first-page":"e27000","DOI":"10.1371\/journal.pone.0027000","article-title":"Differential expression of Wnts after spinal cord contusion injury in adult rats","volume":"6","author":"Fernandez-Martos","year":"2011","journal-title":"PLoS ONE"},{"key":"2026090107091085900_DEV204933C20","doi-asserted-by":"publisher","first-page":"363","DOI":"10.3389\/fphys.2013.00363","article-title":"Nuclear positioning in muscle development and disease","volume":"4","author":"Folker","year":"2013","journal-title":"Front. Physiol."},{"key":"2026090107091085900_DEV204933C21","doi-asserted-by":"publisher","first-page":"e78","DOI":"10.1002\/cpz1.78","article-title":"Streptozotocin-induced diabetic models in mice and rats","volume":"1","author":"Furman","year":"2021","journal-title":"Curr. Protoc."},{"key":"2026090107091085900_DEV204933C22","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/bs.pmbts.2017.11.026","article-title":"Wnt signaling in skeletal muscle development and regeneration","volume":"153","author":"Girardi","year":"2018","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"2026090107091085900_DEV204933C23","doi-asserted-by":"publisher","first-page":"4794","DOI":"10.1038\/s41467-022-32400-2","article-title":"A single WNT enhancer drives specification and regeneration of the Drosophila wing","volume":"13","author":"Gracia-Latorre","year":"2022","journal-title":"Nat. Commun."},{"key":"2026090107091085900_DEV204933C24","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.bone.2015.04.010","article-title":"A distinct regulatory region of the Bmp5 locus activates gene expression following adult bone fracture or soft tissue injury","volume":"77","author":"Guenther","year":"2015","journal-title":"Bone"},{"key":"2026090107091085900_DEV204933C25","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s13395-024-00367-x","article-title":"Wnt7a is required for regeneration of dystrophic skeletal muscle","volume":"14","author":"Gurriaran-Rodriguez","year":"2024","journal-title":"Skelet. Muscle"},{"key":"2026090107091085900_DEV204933C27","doi-asserted-by":"publisher","first-page":"e0147198","DOI":"10.1371\/journal.pone.0147198","article-title":"Comparative study of injury models for studying muscle regeneration in mice","volume":"11","author":"Hardy","year":"2016","journal-title":"PLoS ONE"},{"key":"2026090107091085900_DEV204933C28","doi-asserted-by":"publisher","first-page":"e11588","DOI":"10.7554\/eLife.11588","article-title":"Localized epigenetic silencing of a damage-activated WNT enhancer limits regeneration in mature Drosophila imaginal discs","volume":"5","author":"Harris","year":"2016","journal-title":"eLife"},{"key":"2026090107091085900_DEV204933C29","doi-asserted-by":"publisher","first-page":"e58305","DOI":"10.7554\/eLife.58305","article-title":"Damage-responsive, maturity-silenced enhancers regulate multiple genes that direct regeneration in Drosophila","volume":"9","author":"Harris","year":"2020","journal-title":"eLife"},{"key":"2026090107091085900_DEV204933C30","doi-asserted-by":"publisher","first-page":"116263","DOI":"10.1016\/j.bone.2021.116263","article-title":"Characterization of mouse Bmp5 regulatory injury element in zebrafish wound models","volume":"155","author":"Heller","year":"2022","journal-title":"Bone"},{"key":"2026090107091085900_DEV204933C31","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.devcel.2017.07.009","article-title":"GATA factor-regulated Samd14 enhancer confers red blood cell regeneration and survival in severe anemia","volume":"42","author":"Hewitt","year":"2017","journal-title":"Dev. Cell"},{"key":"2026090107091085900_DEV204933C32","volume-title":"Manipulating the Mouse Embryo: A Laboratory Manual","author":"Hogan","year":"1994","edition":"2nd ed"},{"key":"2026090107091085900_DEV204933C33","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1165\/ajrcmb.24.6.4498","article-title":"Clara cell secretory protein-expressing cells of the airway neuroepithelial body microenvironment include a label-retaining subset and are critical for epithelial renewal after progenitor cell depletion","volume":"24","author":"Hong","year":"2001","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026090107091085900_DEV204933C34","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1186\/1471-2164-13-345","article-title":"Microbial transformation from normal oral microbiota to acute endodontic infections","volume":"13","author":"Hsiao","year":"2012","journal-title":"BMC Genomics"},{"key":"2026090107091085900_DEV204933C35","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.jtcvs.2013.10.039","article-title":"Repair of naphthalene-induced acute tracheal injury by basal cells depends on \u03b2-catenin","volume":"148","author":"Hsu","year":"2014","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"2026090107091085900_DEV204933C36","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1126\/science.1229765","article-title":"C\/EBP transcription factors mediate epicardial activation during heart development and injury","volume":"338","author":"Huang","year":"2012","journal-title":"Science"},{"key":"2026090107091085900_DEV204933C37","doi-asserted-by":"publisher","first-page":"3128","DOI":"10.1242\/dev.139907","article-title":"Wnt\/\u03b2-catenin signaling via Axin2 is required for myogenesis and, together with YAP\/Taz and Tead1, active in IIa\/IIx muscle fibers","volume":"143","author":"Huraskin","year":"2016","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C38","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1128\/MCB.22.4.1172-1183.2002","article-title":"Wnt\/\u03b2-catenin\/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway","volume":"22","author":"Jho","year":"2002","journal-title":"Mol. Cell. Biol."},{"key":"2026090107091085900_DEV204933C39","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1038\/ncb2015","article-title":"Muscle injury activates resident fibro\/adipogenic progenitors that facilitate myogenesis","volume":"12","author":"Joe","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"2026090107091085900_DEV204933C40","doi-asserted-by":"publisher","first-page":"83","DOI":"10.4235\/agmr.19.0012","article-title":"Systemic and local phenotypes of barium chloride induced skeletal muscle injury in mice","volume":"23","author":"Jung","year":"2019","journal-title":"Ann. Geriatr. Med. Res."},{"key":"2026090107091085900_DEV204933C41","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1038\/s41419-024-07173-9","article-title":"Ror2 signaling regulated by differential Wnt proteins determines pathological fate of muscle mesenchymal progenitors","volume":"15","author":"Kamizaki","year":"2024","journal-title":"Cell Death Dis."},{"key":"2026090107091085900_DEV204933C42","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1038\/nature17644","article-title":"Modulation of tissue repair by regeneration enhancer elements","volume":"532","author":"Kang","year":"2016","journal-title":"Nature"},{"key":"2026090107091085900_DEV204933C43","doi-asserted-by":"publisher","first-page":"1438","DOI":"10.3791\/1438","article-title":"LAD-ligation: a murine model of myocardial infarction","volume":"32","author":"Kolk","year":"2009","journal-title":"J. Vis. Exp"},{"key":"2026090107091085900_DEV204933C44","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1242\/dev.105.4.707","article-title":"Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice","volume":"105","author":"Kothary","year":"1989","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C45","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1002\/stem.3319","article-title":"CCAAT\/enhancer-binding protein beta promotes muscle stem cell quiescence through regulation of quiescence-associated genes","volume":"39","author":"Lala-Tabbert","year":"2021","journal-title":"Stem Cells"},{"key":"2026090107091085900_DEV204933C46","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.stem.2009.03.013","article-title":"Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells","volume":"4","author":"Le Grand","year":"2009","journal-title":"Cell Stem Cell"},{"key":"2026090107091085900_DEV204933C47","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1038\/nature05453","article-title":"Genome-wide atlas of gene expression in the adult mouse brain","volume":"445","author":"Lein","year":"2007","journal-title":"Nature"},{"key":"2026090107091085900_DEV204933C48","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s00427-019-00629-5","article-title":"Analysis of the wnt1 regulatory chromosomal landscape","volume":"229","author":"Lekven","year":"2019","journal-title":"Dev. Genes Evol."},{"key":"2026090107091085900_DEV204933C49","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1038\/nm.3869","article-title":"Nilotinib reduces muscle fibrosis in chronic muscle injury by promoting TNF-mediated apoptosis of fibro\/adipogenic progenitors","volume":"21","author":"Lemos","year":"2015","journal-title":"Nat. Med."},{"key":"2026090107091085900_DEV204933C50","doi-asserted-by":"publisher","first-page":"e61616","DOI":"10.3791\/61616","article-title":"Murine excisional wound healing model and histological morphometric wound analysis","volume":"162","author":"Lindsey","year":"2020","journal-title":"J. Vis. Exp."},{"key":"2026090107091085900_DEV204933C51","doi-asserted-by":"publisher","first-page":"35503","DOI":"10.1074\/jbc.M402937200","article-title":"Wnt10b inhibits development of white and brown adipose tissues","volume":"279","author":"Longo","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2026090107091085900_DEV204933C52","doi-asserted-by":"publisher","first-page":"e71084","DOI":"10.1371\/journal.pone.0071084","article-title":"Genomic profiling reveals that transient adipogenic activation is a hallmark of mouse models of skeletal muscle regeneration","volume":"8","author":"Lukjanenko","year":"2013","journal-title":"PLoS ONE"},{"key":"2026090107091085900_DEV204933C53","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.1128\/MCB.22.4.1184-1193.2002","article-title":"Negative feedback loop of Wnt signaling through upregulation of conductin\/axin2 in colorectal and liver tumors","volume":"22","author":"Lustig","year":"2002","journal-title":"Mol. Cell. Biol."},{"key":"2026090107091085900_DEV204933C54","doi-asserted-by":"publisher","first-page":"4047","DOI":"10.1242\/dev.152587","article-title":"Fibroblast growth factors: key players in regeneration and tissue repair","volume":"144","author":"Maddaluno","year":"2017","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C55","first-page":"558","article-title":"Morphology of a naphthalene-induced bronchiolar lesion","volume":"86","author":"Mahvi","year":"1977","journal-title":"Am. J. Pathol."},{"key":"2026090107091085900_DEV204933C57","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/978-3-642-71248-7_26","article-title":"Cardiotoxic actions of doxepin and barium chloride in conscious rabbits","volume":"9","author":"Mattila","year":"1986","journal-title":"Arch. Toxicol. Suppl."},{"key":"2026090107091085900_DEV204933C58","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.ydbio.2008.04.004","article-title":"Three genes control the timing, the site and the size of blastema formation in Drosophila","volume":"319","author":"McClure","year":"2008","journal-title":"Dev. Biol."},{"key":"2026090107091085900_DEV204933C59","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.1038\/s42003-021-02810-x","article-title":"Large-scale integration of single-cell transcriptomic data captures transitional progenitor states in mouse skeletal muscle regeneration","volume":"4","author":"McKellar","year":"2021","journal-title":"Commun. Biol."},{"key":"2026090107091085900_DEV204933C60","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/nbt.1630","article-title":"GREAT improves functional interpretation of cis-regulatory regions","volume":"28","author":"McLean","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"2026090107091085900_DEV204933C61","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1002\/mus.26329","article-title":"Evidence of induced muscle regeneration persists for years in the mouse","volume":"58","author":"Meyer","year":"2018","journal-title":"Muscle Nerve"},{"key":"2026090107091085900_DEV204933C62","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1038\/nmeth.3543","article-title":"CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo","volume":"12","author":"Moreno-Mateos","year":"2015","journal-title":"Nat. Methods"},{"key":"2026090107091085900_DEV204933C63","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.stemcr.2014.06.019","article-title":"Transiently active Wnt\/\u03b2-catenin signaling is not required but must be silenced for stem cell function during muscle regeneration","volume":"3","author":"Murphy","year":"2014","journal-title":"Stem Cell Rep."},{"key":"2026090107091085900_DEV204933C64","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1016\/j.cell.2017.05.016","article-title":"Wnt\/\u03b2-catenin signaling, disease, and emerging therapeutic modalities","volume":"169","author":"Nusse","year":"2017","journal-title":"Cell"},{"key":"2026090107091085900_DEV204933C65","doi-asserted-by":"publisher","first-page":"e1007181","DOI":"10.1371\/journal.pgen.1007181","article-title":"Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies","volume":"14","author":"O'Brien","year":"2018","journal-title":"PLoS Genet."},{"key":"2026090107091085900_DEV204933C66","doi-asserted-by":"publisher","first-page":"100993","DOI":"10.1016\/j.isci.2020.100993","article-title":"Temporal dynamics and heterogeneity of cell populations during skeletal muscle regeneration","volume":"23","author":"Oprescu","year":"2020","journal-title":"iScience"},{"key":"2026090107091085900_DEV204933C67","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1038\/nature25461","article-title":"Enhancer redundancy provides phenotypic robustness in mammalian development","volume":"554","author":"Osterwalder","year":"2018","journal-title":"Nature"},{"key":"2026090107091085900_DEV204933C68","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1002\/jcsm.12259","article-title":"Short-term disuse promotes fatty acid infiltration into skeletal muscle","volume":"9","author":"Pagano","year":"2018","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"2026090107091085900_DEV204933C69","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.devcel.2012.07.008","article-title":"Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks","volume":"23","author":"Park","year":"2012","journal-title":"Dev. Cell"},{"key":"2026090107091085900_DEV204933C70","doi-asserted-by":"publisher","first-page":"1120365","DOI":"10.3389\/fcell.2023.1120365","article-title":"The role of WNT10B in physiology and disease: a 10-year update","volume":"11","author":"Perkins","year":"2023","journal-title":"Front. Cell Dev. Biol."},{"key":"2026090107091085900_DEV204933C71","doi-asserted-by":"publisher","first-page":"17061","DOI":"10.1073\/pnas.0906823106","article-title":"A wound-induced Wnt expression program controls planarian regeneration polarity","volume":"106","author":"Petersen","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026090107091085900_DEV204933C72","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1016\/S0092-8674(03)00437-9","article-title":"Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration","volume":"113","author":"Polesskaya","year":"2003","journal-title":"Cell"},{"key":"2026090107091085900_DEV204933C73","doi-asserted-by":"publisher","first-page":"DEV204458","DOI":"10.1242\/dev.204458","article-title":"A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program","volume":"152","author":"Rao","year":"2025","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C74","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/S0925-4773(01)00452-X","article-title":"Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis","volume":"107","author":"Reddy","year":"2001","journal-title":"Mech. Dev."},{"key":"2026090107091085900_DEV204933C75","doi-asserted-by":"publisher","first-page":"2921","DOI":"10.1038\/s41418-020-0551-y","article-title":"Adipogenesis of skeletal muscle fibro\/adipogenic progenitors is affected by the WNT5a\/GSK3\/\u03b2-catenin axis","volume":"27","author":"Reggio","year":"2020","journal-title":"Cell Death Differ."},{"key":"2026090107091085900_DEV204933C76","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/S0002-9440(10)64727-X","article-title":"Neuroepithelial bodies of pulmonary airways serve as a reservoir of progenitor cells capable of epithelial regeneration","volume":"156","author":"Reynolds","year":"2000","journal-title":"Am. J. Pathol."},{"key":"2026090107091085900_DEV204933C77","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1126\/science.289.5481.950","article-title":"Inhibition of adipogenesis by Wnt signaling","volume":"289","author":"Ross","year":"2000","journal-title":"Science"},{"key":"2026090107091085900_DEV204933C78","doi-asserted-by":"publisher","first-page":"2735","DOI":"10.1242\/dev.125.14.2735","article-title":"Identification of an evolutionarily conserved 110 base-pair cis-acting regulatory sequence that governs Wnt-1 expression in the murine neural plate","volume":"125","author":"Rowitch","year":"1998","journal-title":"Development"},{"key":"2026090107091085900_DEV204933C79","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1007\/s00018-015-1857-7","article-title":"Fat deposition and accumulation in the damaged and inflamed skeletal muscle: cellular and molecular players","volume":"72","author":"Sciorati","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090107091085900_DEV204933C80","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.1172\/JCI122694","article-title":"Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer","volume":"129","author":"Soukup","year":"2019","journal-title":"J. Clin. Invest."},{"key":"2026090107091085900_DEV204933C81","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1146\/annurev-cellbio-120319-114716","article-title":"Beyond casual resemblances: rigorous frameworks for comparing regeneration across species","volume":"37","author":"Srivastava","year":"2021","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"2026090107091085900_DEV204933C82","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1038\/s41592-020-01018-x","article-title":"Cellpose: a generalist algorithm for cellular segmentation","volume":"18","author":"Stringer","year":"2021","journal-title":"Nat. Methods"},{"key":"2026090107091085900_DEV204933C83","doi-asserted-by":"publisher","first-page":"L791","DOI":"10.1152\/ajpcell.1995.269.3.C791","article-title":"Plasticity of airway cell proliferation and gene expression after acute naphthalene injury","volume":"269","author":"Stripp","year":"1995","journal-title":"Am. J. Physiol."},{"key":"2026090107091085900_DEV204933C84","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/s41556-022-00906-y","article-title":"Enhancer selection dictates gene expression responses in remote organs during tissue regeneration","volume":"24","author":"Sun","year":"2022","journal-title":"Nat. Cell Biol."},{"key":"2026090107091085900_DEV204933C85","doi-asserted-by":"publisher","first-page":"e43186","DOI":"10.7554\/eLife.43186","article-title":"Arid3a regulates nephric tubule regeneration via evolutionarily conserved regeneration signal-response enhancers","volume":"8","author":"Suzuki","year":"2019","journal-title":"eLife"},{"key":"2026090107091085900_DEV204933C86","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.neuron.2014.05.033","article-title":"A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain","volume":"83","author":"Thompson","year":"2014","journal-title":"Neuron"},{"key":"2026090107091085900_DEV204933C87","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/ncb2014","article-title":"Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle","volume":"12","author":"Uezumi","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"2026090107091085900_DEV204933C88","doi-asserted-by":"publisher","DOI":"10.1101\/2025.04.11.648333","article-title":"GRHL and PGR control WNT4 expression in the mammary gland via 3D looping of conserved and species-specific enhancers","author":"van de Grift","year":"2025","journal-title":"bioRxiv"},{"key":"2026090107091085900_DEV204933C89","doi-asserted-by":"publisher","first-page":"L800","DOI":"10.1152\/ajplung.1995.269.6.L800","article-title":"Cellular response in naphthalene-induced Clara cell injury and bronchiolar epithelial repair in mice","volume":"269","author":"Van Winkle","year":"1995","journal-title":"Am. J. Physiol."},{"key":"2026090107091085900_DEV204933C90","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1002\/dvdy.10441","article-title":"Identification of the mammary line in mouse by Wnt10b expression","volume":"229","author":"Veltmaat","year":"2004","journal-title":"Dev. Dyn."},{"key":"2026090107091085900_DEV204933C91","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.1091\/mbc.e04-08-0720","article-title":"Wnt10b deficiency promotes coexpression of myogenic and adipogenic programs in myoblasts","volume":"16","author":"Vertino","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"2026090107091085900_DEV204933C92","doi-asserted-by":"publisher","first-page":"1852","DOI":"10.1101\/gr.233098.117","article-title":"Damage-responsive elements in Drosophila regeneration","volume":"28","author":"Vizcaya-Molina","year":"2018","journal-title":"Genome Res."},{"key":"2026090107091085900_DEV204933C93","doi-asserted-by":"publisher","first-page":"4409","DOI":"10.1172\/JCI58097","article-title":"Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury","volume":"121","author":"Volckaert","year":"2011","journal-title":"J. Clin. Invest."},{"key":"2026090107091085900_DEV204933C94","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1016\/j.tcb.2012.07.008","article-title":"Wnt signaling in myogenesis","volume":"22","author":"von Maltzahn","year":"2012","journal-title":"Trends Cell Biol."},{"key":"2026090107091085900_DEV204933C95","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s11010-007-9482-x","article-title":"Adipogenic potential can be activated during muscle regeneration","volume":"304","author":"Wagatsuma","year":"2007","journal-title":"Mol. Cell. Biochem."},{"key":"2026090107091085900_DEV204933C96","doi-asserted-by":"publisher","first-page":"11793","DOI":"10.1038\/s41598-021-91191-6","article-title":"Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle","volume":"11","author":"Waisman","year":"2021","journal-title":"Sci. Rep."},{"key":"2026090107091085900_DEV204933C97","doi-asserted-by":"publisher","first-page":"eaaz3090","DOI":"10.1126\/science.aaz3090","article-title":"Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates","volume":"369","author":"Wang","year":"2020","journal-title":"Science"},{"key":"2026090107091085900_DEV204933C98","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/s41597-024-03114-5","article-title":"Integrative cross-species analysis reveals conserved and unique signatures in fatty skeletal muscles","volume":"11","author":"Wang","year":"2024","journal-title":"Sci. Data"},{"key":"2026090107091085900_DEV204933C99","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1080\/713611034","article-title":"Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model","volume":"33","author":"Weber","year":"2003","journal-title":"Crit. Rev. Toxicol."},{"key":"2026090107091085900_DEV204933C100","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1113\/EP088142","article-title":"Modulation of Klotho expression in injured muscle perturbs Wnt signalling and influences the rate of muscle growth","volume":"105","author":"Welc","year":"2020","journal-title":"Exp. Physiol."},{"key":"2026090107091085900_DEV204933C101","doi-asserted-by":"publisher","first-page":"a008078","DOI":"10.1101\/cshperspect.a008078","article-title":"Wnt signaling and injury repair","volume":"4","author":"Whyte","year":"2012","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2026090107091085900_DEV204933C102","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1007\/978-1-61779-228-1_18","article-title":"Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences","volume":"772","author":"Wittkopp","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"2026090107091085900_DEV204933C103","doi-asserted-by":"publisher","first-page":"2029","DOI":"10.1016\/j.celrep.2019.04.074","article-title":"Mesenchymal stromal cells are required for regeneration and homeostatic maintenance of skeletal muscle","volume":"27","author":"Wosczyna","year":"2019","journal-title":"Cell Rep."},{"key":"2026090107091085900_DEV204933C104","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1038\/s41594-022-00896-3","article-title":"Current challenges in understanding the role of enhancers in disease","volume":"29","author":"Zaugg","year":"2022","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2026090107091085900_DEV204933C105","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1165\/rcmb.2007-0380OC","article-title":"Molecular staging of epithelial maturation using secretory cell-specific genes as markers","volume":"40","author":"Zemke","year":"2009","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026090107091085900_DEV204933C106","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.1002\/hep.30563","article-title":"Tissue repair in the mouse liver following acute carbon tetrachloride depends on injury-induced Wnt\/\u03b2-Catenin Signaling","volume":"69","author":"Zhao","year":"2019","journal-title":"Hepatology"},{"key":"2026090107091085900_DEV204933C107","doi-asserted-by":"publisher","first-page":"1406046","DOI":"10.3389\/fendo.2024.1406046","article-title":"Fatty infiltration in the musculoskeletal system: pathological mechanisms and clinical implications","volume":"15","author":"Zhu","year":"2024","journal-title":"Front. Endocrinol."},{"key":"2026090107091085900_DEV204933C108","doi-asserted-by":"publisher","first-page":"eadh5313","DOI":"10.1126\/sciadv.adh5313","article-title":"An injury-responsive mmp14b enhancer is required for heart regeneration","volume":"9","author":"Zlatanova","year":"2023","journal-title":"Sci. Adv."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.204933\/3674079\/dev204933.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.204933\/3677049\/dev204933.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.204933\/3677049\/dev204933.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:09:18Z","timestamp":1788260958000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/152\/24\/dev204933\/370127\/Deletion-of-an-enhancer-that-controls-Wnt-gene"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,15]]},"references-count":106,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2025,12,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.204933","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.10.01.679323","asserted-by":"object"}]},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,15]]},"article-number":"dev204933"}}