{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:29:32Z","timestamp":1780342172406,"version":"3.54.1"},"reference-count":95,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"crossref","award":["B53D23008280006"],"award-info":[{"award-number":["B53D23008280006"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"crossref","award":["B53D23008270006"],"award-info":[{"award-number":["B53D23008270006"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004462","name":"National Research Council of Italy","doi-asserted-by":"crossref","award":["DSB.AD006.371-InvAt-FOE2022"],"award-info":[{"award-number":["DSB.AD006.371-InvAt-FOE2022"]}],"id":[{"id":"10.13039\/501100004462","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Autosomal recessive primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by reduced brain size and intellectual disability. Mutations in over 30 genes, nearly half linked to centrosome biogenesis or microtubule (MT) dynamics, highlight spindle defects in disease aetiology, yet these alone do not fully explain MCPH. Here, we show that the Drosophila orthologs of ASPM\/MCPH5 (asp) and CENPJ\/MCPH6 (Sas4) contribute to safeguard nuclear architecture and chromatin organization during brain development. Loss of either gene perturbs MT organization and centromere clustering, leading to reduced Lamin and HP1\u03b1 levels, and to deformed nuclear lamina. Mutants also display a global reduction in heterochromatin-associated histone marks, H3K9me2\/3 and H3K27me3, along with an increase in the euchromatin-associated mark H3K4me3 and elevated DNA damage with delayed repair. Notably, inhibiting demethylases with methylstat restores H3K9me3 and nuclear morphology. These findings suggest a previously unreported role for centrosome proteins in regulating chromatin organization, providing new insights into the mechanisms underlying MCPH pathogenesis.<\/jats:p>","DOI":"10.1242\/dev.205125","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:06:56Z","timestamp":1777622816000},"source":"Crossref","is-referenced-by-count":1,"title":["Microcephaly-associated genes\n                    <i>asp<\/i>\n                    and\n                    <i>Sas4<\/i>\n                    influence chromatin organization and nuclear lamina structure in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"153","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9086-132X","authenticated-orcid":false,"given":"Degisew Yinur","family":"Mengistu","sequence":"first","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7229-1693","authenticated-orcid":false,"given":"Marta","family":"Marzullo","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"IBPM CNR 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"Sapienza University of Rome 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6818-1229","authenticated-orcid":false,"given":"Claudia","family":"Pellacani","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"IBPM CNR 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"Sapienza University of Rome 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5431-5161","authenticated-orcid":false,"given":"Marcella","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7183-4360","authenticated-orcid":false,"given":"Marta","family":"Terribili","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Montivero Morales","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7585-3484","authenticated-orcid":false,"given":"Maria Patrizia","family":"Somma","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"IBPM CNR 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]},{"name":"Sapienza University of Rome 2 , c\/o Department of Biology and Biotechnologies \u2018C. Darwin\u2019 , , Rome 00185,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0817-1862","authenticated-orcid":false,"given":"Laura","family":"Ciapponi","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome 1 Department of Biology and Biotechnologies \u2018C. 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