{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T11:42:52Z","timestamp":1792496572356,"version":"4.5.0"},"reference-count":0,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"name":"National Institutes of Health","award":["P30 CA014520"],"award-info":[{"award-number":["P30 CA014520"]}]},{"name":"National Institutes of Health","award":["1S10OD018202-01"],"award-info":[{"award-number":["1S10OD018202-01"]}]},{"name":"National Institutes of Health","award":["1S10RR025483-01"],"award-info":[{"award-number":["1S10RR025483-01"]}]},{"name":"National Institutes of Health","award":["K01DK111790"],"award-info":[{"award-number":["K01DK111790"]}]},{"name":"National Institutes of Health","award":["R01DK128238"],"award-info":[{"award-number":["R01DK128238"]}]},{"name":"National Institutes of Health","award":["R01HL174965"],"award-info":[{"award-number":["R01HL174965"]}]},{"name":"National Institutes of Health","award":["R01HL142998"],"award-info":[{"award-number":["R01HL142998"]}]},{"name":"National Institutes of Health","award":["R56HL142998"],"award-info":[{"award-number":["R56HL142998"]}]},{"name":"American Society of Hematology","award":["Bridge Grant"],"award-info":[{"award-number":["Bridge Grant"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Hematopoietic stem and progenitor cells (HSPCs) are essential for differentiation into all blood cell types. In mammals, the interaction between HSPCs and the fetal liver niche during development is critical for stem cell maturation. Integrin alpha 4 (Itga4) on HSPCs and vascular cell adhesion molecule (Vcam1) on niche cells are critical for HSPC colonization of the fetal liver (FL). Itga4 and Vcam1 also function in the zebrafish equivalent of the FL, the caudal hematopoietic tissue (CHT), however, the specific niche cells that express Vcam1 remain unclear. Using multiple approaches, including fluorescent in situ hybridization, we found Vcam1 is expressed in endothelial cells (ECs) and mesenchymal stromal cells (MSCs), but not macrophages. Time-lapse live imaging of itga4 mutants showed the Itga4-Vcam1 axis is not required for guiding HSPCs into the CHT niche but is required for their retention. Our results show that Itga4 on HSPCs, and Vcam1 on ECs and MSCs, are involved in CHT niche retention.<\/jats:p>","DOI":"10.1242\/dev.205304","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T11:19:11Z","timestamp":1792495151000},"source":"Crossref","is-referenced-by-count":0,"title":["Vcam1 in endothelial and stromal cells regulates hematopoietic stem cell contact with the niche"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6907-4564","authenticated-orcid":false,"given":"Octavia","family":"Santis Larrain","sequence":"first","affiliation":[{"name":"1 Department of Cell and RegenerativeBiology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Alhaj","family":"Kadour","sequence":"additional","affiliation":[{"name":"1 Department of Cell and RegenerativeBiology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sobhika","family":"Agarwala","sequence":"additional","affiliation":[{"name":"1 Department of Cell and RegenerativeBiology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0641-6982","authenticated-orcid":false,"given":"Wantong","family":"Li","sequence":"additional","affiliation":[{"name":"2 The Ohio State University College of Medicine, Department of Internal Medicine, Division of Hematology, Columbus, OH, USA"},{"name":"3 The Ohio State University Comprehensive Cancer Center, James Cancer Hospital and Solove Research Institute, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3168-5423","authenticated-orcid":false,"given":"Bradley W.","family":"Blaser","sequence":"additional","affiliation":[{"name":"2 The Ohio State University College of Medicine, Department of Internal Medicine, Division of Hematology, Columbus, OH, USA"},{"name":"3 The Ohio State University Comprehensive Cancer Center, James Cancer Hospital and Solove Research Institute, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Lasarev","sequence":"additional","affiliation":[{"name":"4 Department of Biostatistics and Medical Informatics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana","family":"Alexandridis","sequence":"additional","affiliation":[{"name":"4 Department of Biostatistics and Medical Informatics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1912-4230","authenticated-orcid":false,"given":"Julian T.","family":"Kamanda","sequence":"additional","affiliation":[{"name":"1 Department of Cell and RegenerativeBiology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Veltri","sequence":"additional","affiliation":[{"name":"5 Bioinformatics Resource Center, University of Wisconsin Biotechnology Center, University of Wisconsin\u2013Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaliun","family":"Enkhbayar","sequence":"additional","affiliation":[{"name":"6 Section of Hematology and Medical Oncology and Center for Regenerative Medicine, Boston University School of Medicine and Boston Medical Center, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliott J.","family":"Hagedorn","sequence":"additional","affiliation":[{"name":"6 Section of Hematology and Medical Oncology and Center for Regenerative Medicine, Boston University School of Medicine and Boston Medical Center, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-4860","authenticated-orcid":false,"given":"Owen J.","family":"Tamplin","sequence":"additional","affiliation":[{"name":"1 Department of Cell and RegenerativeBiology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,10,20]]},"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.205304\/3722062\/dev205304.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.205304\/3722062\/dev205304.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T11:19:12Z","timestamp":1792495152000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/doi\/10.1242\/dev.205304\/373214\/Vcam1-in-endothelial-and-stromal-cells-regulates"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1242\/dev.205304","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]},"article-number":"dev.205304"}}