{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:49:13Z","timestamp":1787021353930,"version":"build-2736575974"},"reference-count":99,"publisher":"The Company of Biologists","issue":"14","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R15-GM117502"],"award-info":[{"award-number":["R15-GM117502"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R35-GM158065"],"award-info":[{"award-number":["R35-GM158065"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008501","name":"East Carolina University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008501","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The steroid hormone ecdysone controls Drosophila ovarian germline stem cell (GSC) maintenance and germ cell differentiation. Prior studies demonstrated that ecdysone regulates germ cell differentiation non-autonomously via the nuclear receptor Ecdysone receptor (EcR) in ovarian somatic cells. Although EcR is also expressed in germ cells, potential direct roles for EcR in the germline independent of the soma have not been examined. Here, we demonstrate that EcR functions autonomously in GSCs and cystoblasts to control germline differentiation. While depletion of EcR from GSCs mildly reduces their maintenance, overexpression of EcR specifically in GSCs and cystoblasts impedes germ cell differentiation, phenotypically resembling loss of function of bag of marbles (bam) and bone morphogenetic protein signaling constitutive activation. We propose that EcR functions as a transcriptional repressor in GSCs and cystoblasts to suppress differentiation but becomes derepressed as the ecdysone titer increases and expression of the co-repressor Smrter decreases in dividing germ cell cysts, providing temporal control over germline differentiation. These data support the model that germ cells integrate signals from multiple cell sources that spatially and temporally control their differentiation in response to local and physiological cues.<\/jats:p>","DOI":"10.1242\/dev.205460","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:27:00Z","timestamp":1783585620000},"source":"Crossref","is-referenced-by-count":2,"title":["<i>Ecdysone receptor<\/i>\n                    autonomously controls germ cell differentiation in the\n                    <i>Drosophila<\/i>\n                    ovary"],"prefix":"10.1242","volume":"153","author":[{"given":"Lauren E.","family":"Jung","sequence":"first","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandria I.","family":"Warren","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhong","family":"Yin","sequence":"additional","affiliation":[{"name":"Brody School of Medicine 2 Department of Pathology and Laboratory Medicine , , Greenville, NC 27834 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Huang","sequence":"additional","affiliation":[{"name":"Brody School of Medicine 2 Department of Pathology and Laboratory Medicine , , Greenville, NC 27834 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison C.","family":"Simmons","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha I.","family":"McDonald","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay A.","family":"Swain","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria E.","family":"Garrido","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Phipps","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BiClaireline","family":"Cesar","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle S.","family":"Finger","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Sun","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Green Pesticide, South China Agricultural University 3 , 510642 Guangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd G.","family":"Nystul","sequence":"additional","affiliation":[{"name":"University of California San Francisco 4 Departments of Anatomy and OB\/Gyn , , San Francisco, CA 94143 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6934-9302","authenticated-orcid":false,"given":"Elizabeth T.","family":"Ables","sequence":"additional","affiliation":[{"name":"East Carolina University 1 Department of Biology , , Greenville, NC 27858 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"2026072905173294500_DEV205460C1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1016\/j.stem.2010.10.001","article-title":"The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila","volume":"7","author":"Ables","year":"2010","journal-title":"Cell Stem Cell"},{"key":"2026072905173294500_DEV205460C2","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1242\/dev.088583","article-title":"Cyclin E controls Drosophila female germline stem cell maintenance independently of its role in proliferation by modulating responsiveness to niche signals","volume":"140","author":"Ables","year":"2013","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C3","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.ydbio.2015.01.013","article-title":"Ecdysone response gene E78 controls ovarian germline stem cell niche formation and follicle survival in Drosophila","volume":"400","author":"Ables","year":"2015","journal-title":"Dev. Biol."},{"key":"2026072905173294500_DEV205460C4","doi-asserted-by":"publisher","first-page":"2629","DOI":"10.1534\/g3.116.028951","article-title":"A genetic mosaic screen reveals ecdysone-responsive genes regulating Drosophila oogenesis","volume":"6","author":"Ables","year":"2016","journal-title":"G3"},{"key":"2026072905173294500_DEV205460C5","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1038\/nmeth.4463","article-title":"SCENIC: single-cell regulatory network inference and clustering","volume":"14","author":"Aibar","year":"2017","journal-title":"Nat. Methods"},{"key":"2026072905173294500_DEV205460C6","doi-asserted-by":"publisher","first-page":"e1006123","DOI":"10.1371\/journal.pgen.1006123","article-title":"Mating-induced increase in germline stem cells via the neuroendocrine system in female Drosophila","volume":"12","author":"Ameku","year":"2016","journal-title":"PLoS Genet."},{"key":"2026072905173294500_DEV205460C7","doi-asserted-by":"publisher","first-page":"2540","DOI":"10.1101\/gad.1342605","article-title":"The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis","volume":"19","author":"Badenhorst","year":"2005","journal-title":"Genes Dev."},{"key":"2026072905173294500_DEV205460C8","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1016\/j.ydbio.2007.01.029","article-title":"The axon guidance gene lola is required for programmed cell death in the Drosophila ovary","volume":"304","author":"Bass","year":"2007","journal-title":"Dev. Biol."},{"key":"2026072905173294500_DEV205460C9","doi-asserted-by":"publisher","first-page":"jkab003","DOI":"10.1093\/g3journal\/jkab003","article-title":"Orphan nuclear receptor ftz-f1 (NR5A3) promotes egg chamber survival in the Drosophila ovary","volume":"11","author":"Beachum","year":"2021","journal-title":"G3"},{"key":"2026072905173294500_DEV205460C10","doi-asserted-by":"publisher","first-page":"RP94631","DOI":"10.7554\/eLife.94631","article-title":"OVO positively regulates essential maternal pathways by binding near the transcriptional start sites in the Drosophila female germline","volume":"13","author":"Benner","year":"2024","journal-title":"eLife"},{"key":"2026072905173294500_DEV205460C98","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.1038\/s41587-020-0591-3","article-title":"Generalizing RNA velocity to transient cell states through dynamical modeling","volume":"38","author":"Bergen","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2026072905173294500_DEV205460C11","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/s10555-024-10171-0","article-title":"Dynamic interplay of nuclear receptors in tumor cell plasticity and drug resistance: Shifting gears in malignant transformations and applications in cancer therapeutics","volume":"43","author":"BharathwajChetty","year":"2024","journal-title":"Cancer Metastasis Rev."},{"key":"2026072905173294500_DEV205460C12","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.molcel.2017.09.031","article-title":"CBP regulates recruitment and release of promoter-proximal RNA polymerase II","volume":"68","author":"Boija","year":"2017","journal-title":"Mol. Cell"},{"key":"2026072905173294500_DEV205460C13","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1038\/nn.2700","article-title":"ftz-f1 and Hr39 opposing roles on EcR expression during Drosophila mushroom body neuron remodeling","volume":"14","author":"Boulanger","year":"2011","journal-title":"Nat. Neurosci."},{"key":"2026072905173294500_DEV205460C14","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.semcdb.2022.06.004","article-title":"Dynamic regulation of ribosome levels and translation during development","volume":"136","author":"Breznak","year":"2023","journal-title":"Semin. Cell Dev. Biol."},{"key":"2026072905173294500_DEV205460C15","doi-asserted-by":"publisher","first-page":"4581","DOI":"10.1242\/dev.126.20.4581","article-title":"Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila","volume":"126","author":"Buszczak","year":"1999","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C16","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1534\/genetics.106.065961","article-title":"The carnegie protein trap library: a versatile tool for Drosophila developmental studies","volume":"175","author":"Buszczak","year":"2007","journal-title":"Genetics"},{"key":"2026072905173294500_DEV205460C99","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1038\/s41586-019-0969-x","article-title":"The single-cell transcriptional landscape of mammalian organogenesis","volume":"566","author":"Cao","year":"2019","journal-title":"Nature"},{"key":"2026072905173294500_DEV205460C17","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1091\/mbc.e12-04-0267","article-title":"Ash2 acts as an ecdysone receptor coactivator by stabilizing the histone methyltransferase Trr","volume":"24","author":"Carbonell","year":"2013","journal-title":"Mol. Biol. Cell"},{"key":"2026072905173294500_DEV205460C18","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.devcel.2016.02.010","article-title":"Repression of pumilio protein expression by Rbfox1 promotes germ cell differentiation","volume":"36","author":"Carreira-Rosario","year":"2016","journal-title":"Dev. Cell"},{"key":"2026072905173294500_DEV205460C19","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1242\/dev.01076","article-title":"Germline stem cell number in the Drosophila ovary is regulated by redundant mechanisms that control Dpp signaling","volume":"131","author":"Casanueva","year":"2004","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C20","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1242\/dev.00205","article-title":"EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue","volume":"130","author":"Cherbas","year":"2003","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C21","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s13008-021-00073-w","article-title":"Asymmetric cell division of mammary stem cells","volume":"16","author":"Chhabra","year":"2021","journal-title":"Cell Div."},{"key":"2026072905173294500_DEV205460C22","doi-asserted-by":"publisher","first-page":"1848","DOI":"10.1016\/j.devcel.2021.05.018","article-title":"Niches that regulate stem cells and hematopoiesis in adult bone marrow","volume":"56","author":"Comazzetto","year":"2021","journal-title":"Dev. Cell"},{"key":"2026072905173294500_DEV205460C23","doi-asserted-by":"publisher","first-page":"e361","DOI":"10.1002\/wdev.361","article-title":"Cellular mechanisms of epithelial stem cell self-renewal and differentiation during homeostasis and repair","volume":"9","author":"Das","year":"2020","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"2026072905173294500_DEV205460C24","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.ydbio.2012.11.004","article-title":"The transcriptional regulator lola is required for stem cell maintenance and germ cell differentiation in the Drosophila testis","volume":"373","author":"Davies","year":"2013","journal-title":"Dev. Biol."},{"key":"2026072905173294500_DEV205460C25","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1038\/s41580-022-00568-6","article-title":"Regulation of adult stem cell quiescence and its functions in the maintenance of tissue integrity","volume":"24","author":"de Morree","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026072905173294500_DEV205460C26","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1534\/genetics.118.300874","article-title":"Efficient expression of genes in the drosophila germline using a UAS promoter free of interference by Hsp70 piRNAs","volume":"209","author":"DeLuca","year":"2018","journal-title":"Genetics"},{"key":"2026072905173294500_DEV205460C27","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1534\/genetics.119.300234","article-title":"Local and physiological control of germline stem cell lineages in Drosophila melanogaster","volume":"213","author":"Drummond-Barbosa","year":"2019","journal-title":"Genetics"},{"key":"2026072905173294500_DEV205460C28","doi-asserted-by":"publisher","first-page":"6359","DOI":"10.1073\/pnas.91.14.6359","article-title":"DNA-binding and trans-activation properties of Drosophila E2F and DP proteins","volume":"91","author":"Dynlacht","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026072905173294500_DEV205460C29","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/ncb1874","article-title":"Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila","volume":"11","author":"Fichelson","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"2026072905173294500_DEV205460C30","doi-asserted-by":"publisher","first-page":"4253","DOI":"10.1242\/dev.127.19.4253","article-title":"The fusome organizes the microtubule network during oocyte differentiation in Drosophila","volume":"127","author":"Grieder","year":"2000","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C31","doi-asserted-by":"publisher","first-page":"e1007203","DOI":"10.1371\/journal.pgen.1007203","article-title":"Chinmo prevents transformer alternative splicing to maintain male sex identity","volume":"14","author":"Grmai","year":"2018","journal-title":"PLoS Genet"},{"key":"2026072905173294500_DEV205460C32","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/S0022-1910(99)00038-4","article-title":"Ecdysteroid titers in mated and unmated Drosophila melanogaster females","volume":"45","author":"Harshman","year":"1999","journal-title":"J. Insect Physiol."},{"key":"2026072905173294500_DEV205460C33","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1038\/nature25744","article-title":"Mechanical regulation of stem-cell differentiation by the stretch-activated Piezo channel","volume":"555","author":"He","year":"2018","journal-title":"Nature"},{"key":"2026072905173294500_DEV205460C34","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.ydbio.2017.07.001","article-title":"Temporal remodeling of the cell cycle accompanies differentiation in the Drosophila germline","volume":"429","author":"Hinnant","year":"2017","journal-title":"Dev. Biol."},{"key":"2026072905173294500_DEV205460C35","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3389\/fcell.2020.00019","article-title":"Coordinating proliferation, polarity, and cell fate in the Drosophila female germline","volume":"8","author":"Hinnant","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"2026072905173294500_DEV205460C36","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/0012-1606(77)90128-2","article-title":"Ecdysone titers during postembryonic development of Drosophila melanogaster","volume":"60","author":"Hodgetts","year":"1977","journal-title":"Dev. Biol."},{"key":"2026072905173294500_DEV205460C37","doi-asserted-by":"publisher","first-page":"a040840","DOI":"10.1101\/cshperspect.a040840","article-title":"Building and maintaining the skin","volume":"14","author":"Hsu","year":"2022","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2026072905173294500_DEV205460C38","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1210\/me.2002-0287","article-title":"Transcription activation by the ecdysone receptor (EcR\/USP): identification of activation functions","volume":"17","author":"Hu","year":"2003","journal-title":"Mol. Endocrinol."},{"key":"2026072905173294500_DEV205460C39","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.molcel.2011.07.033","article-title":"Ecdysone- and NO-mediated gene regulation by competing EcR\/Usp and E75A nuclear receptors during Drosophila development","volume":"44","author":"Johnston","year":"2011","journal-title":"Mol. Cell"},{"key":"2026072905173294500_DEV205460C40","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1016\/j.bbrc.2008.05.003","article-title":"Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor","volume":"371","author":"Kimura","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026072905173294500_DEV205460C41","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.neuron.2011.08.003","article-title":"Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila","volume":"72","author":"Kirilly","year":"2011","journal-title":"Neuron"},{"key":"2026072905173294500_DEV205460C42","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1242\/bio.201410553","article-title":"Soma influences GSC progeny differentiation via the cell adhesion-mediated steroid-let-7-Wingless signaling cascade that regulates chromatin dynamics","volume":"4","author":"K\u00f6nig","year":"2015","journal-title":"Biol. Open"},{"key":"2026072905173294500_DEV205460C43","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1038\/emboj.2011.73","article-title":"Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation","volume":"30","author":"K\u00f6nig","year":"2011","journal-title":"EMBO J."},{"key":"2026072905173294500_DEV205460C44","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1101\/gad.351402.123","article-title":"Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis","volume":"38","author":"Kotb","year":"2024","journal-title":"Genes Dev."},{"key":"2026072905173294500_DEV205460C45","doi-asserted-by":"publisher","first-page":"1648","DOI":"10.1038\/s44318-026-00697-0","article-title":"TORC1-dependent translation drives chromatin remodeling during the germ-cell-to-maternal transition in Drosophila","volume":"45","author":"Kotb","year":"2026"},{"key":"2026072905173294500_DEV205460C46","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/978-1-4939-2851-4_4","article-title":"Genetic mosaic analysis of stem cell lineages in the Drosophila ovary","volume":"1328","author":"Laws","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"2026072905173294500_DEV205460C47","doi-asserted-by":"publisher","first-page":"112007","DOI":"10.1016\/j.celrep.2023.112007","article-title":"The Integrator complex desensitizes cellular response to TGF-\u03b2\/BMP signaling","volume":"42","author":"Liu","year":"2023","journal-title":"Cell Rep."},{"key":"2026072905173294500_DEV205460C48","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.devcel.2011.06.018","article-title":"Drosophila stem cell niches: a decade of discovery suggests a unified view of stem cell regulation","volume":"21","author":"Losick","year":"2011","journal-title":"Dev. Cell"},{"key":"2026072905173294500_DEV205460C49","doi-asserted-by":"publisher","first-page":"119074","DOI":"10.1016\/j.gep.2019.119074","article-title":"Novel cis-regulatory regions in ecdysone responsive genes are sufficient to promote gene expression in Drosophila ovarian cells","volume":"34","author":"McDonald","year":"2019","journal-title":"Gene Expr. Patterns"},{"key":"2026072905173294500_DEV205460C50","doi-asserted-by":"publisher","first-page":"2242","DOI":"10.1101\/gad.4.12b.2242","article-title":"bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis","volume":"4","author":"McKearin","year":"1990","journal-title":"Genes Dev."},{"key":"2026072905173294500_DEV205460C51","doi-asserted-by":"publisher","first-page":"e46109","DOI":"10.1371\/journal.pone.0046109","article-title":"Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry","volume":"7","author":"Morris","year":"2012","journal-title":"PLoS ONE"},{"key":"2026072905173294500_DEV205460C52","doi-asserted-by":"publisher","first-page":"102148","DOI":"10.1016\/j.gde.2023.102148","article-title":"Timing Drosophila development through steroid hormone action","volume":"84","author":"Morrow","year":"2024","journal-title":"Curr. Opin. Genet. Dev."},{"key":"2026072905173294500_DEV205460C53","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1038\/nature12932","article-title":"Oestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy","volume":"505","author":"Nakada","year":"2014","journal-title":"Nature"},{"key":"2026072905173294500_DEV205460C54","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1242\/dev.121.2.589","article-title":"Nubbin encodes a POU-domain protein required for proximal-distal patterning in the Drosophila wing","volume":"121","author":"Ng","year":"1995","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C55","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/nmeth.1592","article-title":"A genome-scale shRNA resource for transgenic RNAi in Drosophila","volume":"8","author":"Ni","year":"2011","journal-title":"Nat. Methods"},{"key":"2026072905173294500_DEV205460C56","doi-asserted-by":"publisher","first-page":"e72666","DOI":"10.7554\/eLife.72666","article-title":"Ecdysone coordinates plastic growth with robust pattern in the developing wing","volume":"11","author":"Nogueira Alves","year":"2022","journal-title":"eLife"},{"key":"2026072905173294500_DEV205460C57","doi-asserted-by":"publisher","first-page":"eadd1884","DOI":"10.1126\/science.add1884","article-title":"Coordinated control of neuronal differentiation and wiring by sustained transcription factors","volume":"378","author":"\u00d6zel","year":"2022","journal-title":"Science"},{"key":"2026072905173294500_DEV205460C58","doi-asserted-by":"publisher","first-page":"iyad211","DOI":"10.1093\/genetics\/iyad211","article-title":"FlyBase: updates to the Drosophila genes and genomes database","volume":"227","author":"\u00d6zt\u00fcrk-\u00c7olak","year":"2024","journal-title":"Genetics"},{"key":"2026072905173294500_DEV205460C59","doi-asserted-by":"publisher","first-page":"RP90509","DOI":"10.7554\/eLife.90509","article-title":"Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells","volume":"12","author":"Pang","year":"2023","journal-title":"eLife"},{"key":"2026072905173294500_DEV205460C60","doi-asserted-by":"publisher","first-page":"2128","DOI":"10.1016\/j.devcel.2023.08.032","article-title":"The Drosophila ecdysone receptor promotes or suppresses proliferation according to ligand level","volume":"58","author":"Perez-Mockus","year":"2023","journal-title":"Dev. Cell"},{"key":"2026072905173294500_DEV205460C61","doi-asserted-by":"publisher","first-page":"e3001660","DOI":"10.1371\/journal.pbio.3001660","article-title":"A juxtamembrane basolateral targeting motif regulates signaling through a TGF-\u03b2 pathway receptor in Drosophila","volume":"20","author":"Peterson","year":"2022","journal-title":"PLoS Biol."},{"key":"2026072905173294500_DEV205460C62","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/978-1-0716-2970-3_4","article-title":"Utilizing the FLP-out system for clonal RNAi analysis in the adult Drosophila ovary","volume":"2626","author":"Phipps","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"2026072905173294500_DEV205460C63","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1002\/dvdy.24359","article-title":"Expression of the Drosophila homeobox gene, Distal-less, supports an ancestral role in neural development","volume":"245","author":"Plavicki","year":"2016","journal-title":"Dev. Dyn."},{"key":"2026072905173294500_DEV205460C64","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1093\/icb\/33.3.340","article-title":"Hormone receptors and the regulation of insect metamorphosis","volume":"3","author":"Riddiford","year":"1993","journal-title":"Receptor"},{"key":"2026072905173294500_DEV205460C65","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/S0925-4773(98)00157-9","article-title":"Gal4 in the Drosophila female germline","volume":"78","author":"R\u00f8rth","year":"1998","journal-title":"Mech. Dev."},{"key":"2026072905173294500_DEV205460C66","doi-asserted-by":"publisher","first-page":"5628","DOI":"10.1038\/s41467-020-19361-0","article-title":"A single-cell atlas and lineage analysis of the adult Drosophila ovary","volume":"11","author":"Rust","year":"2020","journal-title":"Nat. Commun."},{"key":"2026072905173294500_DEV205460C67","doi-asserted-by":"publisher","first-page":"1591","DOI":"10.1038\/s44318-024-00070-z","article-title":"Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation","volume":"43","author":"Samuels","year":"2024","journal-title":"EMBO J."},{"key":"2026072905173294500_DEV205460C68","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.stem.2015.11.004","article-title":"Regulation of ribosome biogenesis and protein synthesis controls germline stem cell differentiation","volume":"18","author":"Sanchez","year":"2016","journal-title":"Cell Stem Cell"},{"key":"2026072905173294500_DEV205460C69","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.jsbmb.2011.01.013","article-title":"The N-terminus of ecdysteroid receptor isoforms and ultraspiracle interacts with different ecdysteroid response elements in a sequence specific manner to modulate transcriptional activity","volume":"124","author":"Schauer","year":"2011","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"2026072905173294500_DEV205460C70","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1016\/S0925-4773(03)00134-5","article-title":"Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor","volume":"120","author":"Schubiger","year":"2003","journal-title":"Mech. Dev."},{"key":"2026072905173294500_DEV205460C71","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1038\/nature02080","article-title":"Methylation at lysine 4 of histone H3 in ecdysone-dependent development of Drosophila","volume":"426","author":"Sedkov","year":"2003","journal-title":"Nature"},{"key":"2026072905173294500_DEV205460C72","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1016\/j.cub.2020.11.053","article-title":"A progressive somatic cell niche regulates germline cyst differentiation in the Drosophila ovary","volume":"31","author":"Shi","year":"2021","journal-title":"Curr. Biol."},{"key":"2026072905173294500_DEV205460C73","doi-asserted-by":"publisher","first-page":"e0167283","DOI":"10.1371\/journal.pone.0167283","article-title":"Regulation of gonad morphogenesis in Drosophila melanogaster by BTB family transcription factors","volume":"11","author":"Silva","year":"2016","journal-title":"PLoS ONE"},{"key":"2026072905173294500_DEV205460C74","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.semcdb.2013.10.002","article-title":"Nuclear receptors as regulators of stem cell and cancer stem cell metabolism","volume":"24","author":"Simandi","year":"2013","journal-title":"Semin. Cell Dev. Biol."},{"key":"2026072905173294500_DEV205460C75","doi-asserted-by":"publisher","first-page":"1938","DOI":"10.1101\/gr.274340.120","article-title":"A single-cell atlas reveals unanticipated cell type complexity in Drosophila ovaries","volume":"31","author":"Slaidina","year":"2021","journal-title":"Genome Res."},{"key":"2026072905173294500_DEV205460C76","doi-asserted-by":"publisher","first-page":"e83230","DOI":"10.7554\/eLife.83230","article-title":"Conservation of oocyte development in germline cysts from Drosophila to mouse","volume":"11","author":"Spradling","year":"2022","journal-title":"eLife"},{"key":"2026072905173294500_DEV205460C77","doi-asserted-by":"publisher","first-page":"e56475","DOI":"10.15252\/embr.202256475","article-title":"Single-cell RNA sequencing identifies eggplant as a regulator of germ cell development in Drosophila","volume":"24","author":"Sun","year":"2023","journal-title":"EMBO Rep."},{"key":"2026072905173294500_DEV205460C78","doi-asserted-by":"publisher","first-page":"2025.2010.2014.682265","DOI":"10.1101\/2025.10.14.682265","article-title":"Single-cell transcriptomic profiling of bam mutant tumor reveals germline heterogeneity and gcrf1 as a modulator in Drosophila germ cells","author":"Sun","year":"2025","journal-title":"bioRxiv"},{"key":"2026072905173294500_DEV205460C79","doi-asserted-by":"publisher","first-page":"iyab031","DOI":"10.1093\/genetics\/iyab031","article-title":"Drosophila MOV10 regulates the termination of midgut regeneration","volume":"218","author":"Takemura","year":"2021","journal-title":"Genetics"},{"key":"2026072905173294500_DEV205460C80","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1016\/0092-8674(93)90359-X","article-title":"Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms","volume":"73","author":"Talbot","year":"1993","journal-title":"Cell"},{"key":"2026072905173294500_DEV205460C81","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.redox.2017.12.002","article-title":"Small Maf functions in the maintenance of germline stem cells in the Drosophila testis","volume":"15","author":"Tan","year":"2018","journal-title":"Redox Biol."},{"key":"2026072905173294500_DEV205460C82","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1038\/ncb3165","article-title":"ATP synthase promotes germ cell differentiation independent of oxidative phosphorylation","volume":"17","author":"Teixeira","year":"2015","journal-title":"Nat. Cell Biol."},{"key":"2026072905173294500_DEV205460C83","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/0168-9525(96)10032-9","article-title":"Flies on steroids--Drosophila metamorphosis and the mechanisms of steroid hormone action","volume":"12","author":"Thummel","year":"1996","journal-title":"Trends Genet."},{"key":"2026072905173294500_DEV205460C84","doi-asserted-by":"publisher","first-page":"9893","DOI":"10.1073\/pnas.1900343116","article-title":"Direct and widespread role for the nuclear receptor EcR in mediating the response to ecdysone in Drosophila","volume":"116","author":"Uyehara","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026072905173294500_DEV205460C85","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1101\/gad.298182.117","article-title":"Hormone-dependent control of developmental timing through regulation of chromatin accessibility","volume":"31","author":"Uyehara","year":"2017","journal-title":"Genes Dev."},{"key":"2026072905173294500_DEV205460C86","doi-asserted-by":"publisher","first-page":"e2208935119","DOI":"10.1073\/pnas.2208935119","article-title":"Opportunistic binding of EcR to open chromatin drives tissue-specific developmental responses","volume":"119","author":"Uyehara","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026072905173294500_DEV205460C87","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/S0960-9822(98)70091-0","article-title":"Regulation of zygotic gene expression in Drosophila primordial germ cells","volume":"8","author":"Van Doren","year":"1998","journal-title":"Curr. Biol."},{"key":"2026072905173294500_DEV205460C88","doi-asserted-by":"publisher","first-page":"dev199716","DOI":"10.1242\/dev.199716","article-title":"Live imaging of the Drosophila ovarian niche shows spectrosome and centrosome dynamics during asymmetric germline stem cell division","volume":"148","author":"Villa-Fombuena","year":"2021","journal-title":"Development"},{"key":"2026072905173294500_DEV205460C89","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/j.cub.2005.02.016","article-title":"The division of Drosophila germline stem cells and their precursors requires a specific cyclin","volume":"15","author":"Wang","year":"2005","journal-title":"Curr. Biol."},{"key":"2026072905173294500_DEV205460C90","doi-asserted-by":"publisher","first-page":"e96933","DOI":"10.1371\/journal.pone.0096933","article-title":"Luna, a Drosophila KLF6\/KLF7, is maternally required for synchronized nuclear and centrosome cycles in the preblastoderm embryo","volume":"9","author":"Weber","year":"2014","journal-title":"PLoS ONE"},{"key":"2026072905173294500_DEV205460C91","doi-asserted-by":"publisher","first-page":"1876","DOI":"10.1002\/pro.3496","article-title":"The nuclear receptor superfamily: a structural perspective","volume":"27","author":"Weikum","year":"2018","journal-title":"Protein Sci."},{"key":"2026072905173294500_DEV205460C92","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/978-1-0716-2970-3_7","article-title":"Visualizing fusome morphology via tubulin immunofluorescence in drosophila ovarian germ cells","volume":"2626","author":"Williams","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"2026072905173294500_DEV205460C93","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/S0092-8674(00)81424-5","article-title":"decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary","volume":"94","author":"Xie","year":"1998","journal-title":"Cell"},{"key":"2026072905173294500_DEV205460C94","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1016\/j.cell.2016.02.041","article-title":"Hardwiring stem cell communication through tissue structure","volume":"164","author":"Xin","year":"2016","journal-title":"Cell"},{"key":"2026072905173294500_DEV205460C95","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1146\/annurev-ento-120811-153608","article-title":"Ecdysone control of developmental transitions: lessons from Drosophila research","volume":"58","author":"Yamanaka","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"2026072905173294500_DEV205460C96","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1038\/s41419-022-05195-9","article-title":"A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis","volume":"13","author":"Zhao","year":"2022","journal-title":"Cell Death Dis."},{"key":"2026072905173294500_DEV205460C97","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.ydbio.2025.05.005","article-title":"Estrogen-related receptor is required in adult Drosophila females for germline stem cell maintenance","volume":"524","author":"Zike","year":"2025","journal-title":"Dev. Biol."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.205460\/3707544\/dev205460.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.205460\/3710622\/dev205460.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.205460\/3710622\/dev205460.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T09:17:44Z","timestamp":1785316664000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/153\/14\/dev205460\/372411\/Ecdysone-receptor-autonomously-controls-germ-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":99,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.205460","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]},"article-number":"dev205460"}}