{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:07:23Z","timestamp":1784819243429,"version":"3.55.0"},"reference-count":82,"publisher":"The Company of Biologists","issue":"14","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-2\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFC2702502"],"award-info":[{"award-number":["2022YFC2702502"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In mammals, the epididymis is composed of pseudostratified epithelium that forms the post-testicular milieu for spermatozoa. To date, the role of epithelial cells in shaping the luminal microenvironment for spermatozoa remains poorly understood. Here, we generated a conditional knockout mouse model of Nudt21 in the epididymis. NUDT21 is a key component of mammalian cleavage factor I (CFIm) that participates in the 3\u2032 end processing of pre-mRNAs. We found that Nudt21 deletion occurred mainly in principal cells of the corpus and the cauda. The loss of NUDT21 in principal cells resulted in male infertility, owing to sperm abnormalities present in the cauda. Pseudotime analysis revealed that the differentiation of Nudt21-null principal cells was blocked, preventing them from exerting their physiological function. Amid transcripts with shortened 3\u2032 untranslated regions (3\u2032UTRs) after Nudt21 deletion, Dicer1 transcripts not only had shortened 3\u2032UTR, but also skipped exon 1, resulting in downregulation of full-length DICER1 protein. Together, these results demonstrate the essential role of CFIm-mediated 3\u2032UTR dynamics in preserving sperm integrity and provide insights into the intricate communication between the epididymal epithelium and spermatozoa.<\/jats:p>","DOI":"10.1242\/dev.205483","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T07:08:12Z","timestamp":1782889692000},"source":"Crossref","is-referenced-by-count":0,"title":["NUDT21 regulates 3\u2032UTR dynamics in epididymal principal cells to preserve sperm integrity"],"prefix":"10.1242","volume":"153","author":[{"given":"Jinzhao","family":"Zhou","sequence":"first","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cai","sequence":"additional","affiliation":[{"name":"University of Zurich 2 Department of Reproductive Endocrinology , , Wagistrasse 14, Schlieren 8952 ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wei","sequence":"additional","affiliation":[{"name":"Chengdu Genepre Technology Co., Ltd 3 , Tianfu Avenue North 1480, Chengdu 610095 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Ling","sequence":"additional","affiliation":[{"name":"Chengdu Genepre Technology Co., Ltd 3 , Tianfu Avenue North 1480, Chengdu 610095 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenpei","family":"Xiang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4086-3321","authenticated-orcid":false,"given":"Huaibiao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology 1 , Hangkong Road 13, Wuhan 430030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"2026072310551107600_DEV205483C1","doi-asserted-by":"publisher","first-page":"e50895","DOI":"10.7554\/eLife.50895","article-title":"Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation","volume":"9","author":"Alcott","year":"2020","journal-title":"eLife"},{"key":"2026072310551107600_DEV205483C2","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1093\/humrep\/deac015","article-title":"Sperm acquire epididymis-derived proteins through epididymosomes","volume":"37","author":"Barrachina","year":"2022","journal-title":"Hum. 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