{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:05:22Z","timestamp":1789729522585,"version":"4.0.1"},"reference-count":77,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-2\/"}],"funder":[{"name":"H2020 Marie Sk\u0142odowska-Curie Actions","award":["766053"],"award-info":[{"award-number":["766053"]}]},{"name":"Agence Nationale de la Recherche","award":["ANR-21-CE13-0024 \u201cMOPSEA\u201d"],"award-info":[{"award-number":["ANR-21-CE13-0024 \u201cMOPSEA\u201d"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Adult hydrozoan cnidarians undergo extensive tissue turnover, generating neural cell types that include nematocytes (stinging cells) and gland cells from interstitial stem cells (i-cells) expressing stemness proteins such as Piwi and Nanos. The contribution of i-cells during embryogenesis, however, has been unclear. We address neural cell origins during development of the Clytia hemisphaerica planula larva. In situ hybridisation of nematogenesis genes revealed that Piwi\/Nanos1-expressing cells generate a substantial pool of nematoblasts (undifferentiated nematocytes) within the gastrula presumptive endoderm, in line with scRNAseq clustering analysis. Some neurogenic and neuronal markers, however, showed a distinct expression profile, developing within a basal layer of the aboral\/lateral ectoderm during gastrulation. Embryo bisection and lineage-tracing experiments support the hypothesis that neurosensory and secretory cell types can be generated from gastrula ectoderm, while nematoblasts and at least some RFamide-expressing ganglionic cells are derived from i-cells. Knockdown and inhibitor treatments revealed steps in neuron and nematocyte development regulated by Wnt-\u03b2-catenin. We propose that two distinct neurogenesis pathways operate during Clytia embryogenesis: one involving ectodermal delamination, and one generating many nematoblasts and some neurons from i-cell-like precursors.<\/jats:p>","DOI":"10.1242\/dev.205645","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:33:01Z","timestamp":1789727581000},"source":"Crossref","is-referenced-by-count":0,"title":["Dual origins for neural cells during development of the\n                    <i>Clytia<\/i>\n                    planula larva"],"prefix":"10.1242","volume":"153","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3562-7992","authenticated-orcid":false,"given":"Antonella","family":"Ruggiero","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1817-6891","authenticated-orcid":false,"given":"Anna","family":"Ferraioli","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2717-6925","authenticated-orcid":false,"given":"Sandra","family":"Chevalier","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4073-0633","authenticated-orcid":false,"given":"Pascal","family":"Lap\u00e9bie","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Girard","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7846-4954","authenticated-orcid":false,"given":"Richard R.","family":"Copley","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3806-3408","authenticated-orcid":false,"given":"Tsuyoshi","family":"Momose","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5422-6318","authenticated-orcid":false,"given":"Carine","family":"Barreau","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9264-2585","authenticated-orcid":false,"given":"Evelyn","family":"Houliston","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS , Laboratoire de Biologie du D\u00e9veloppement de Villefranche-sur-mer (LBDV), 06230 Villefranche-sur-mer ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"2026091806325660100_DEV205645C1","doi-asserted-by":"publisher","first-page":"20150286","DOI":"10.1098\/rstb.2015.0286","article-title":"Gastric pouches and the mucociliary sole: setting the stage for nervous system evolution","volume":"370","author":"Arendt","year":"2015","journal-title":"Philos. 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