{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:24:26Z","timestamp":1791188666333,"version":"4.1.0"},"reference-count":36,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":3059,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Summary<\/jats:title><jats:p>Interleukin-1, the 'gate-keeper' of inflammation, is the apical cytokine in a signalling cascade that drives the early response to injury or infection. Expression, processing and secretion of IL-1 is tightly controlled, whilst dysregulated IL-1 signalling has been implicated in a number of pathologies ranging from atherosclerosis to complications of infection. Our understanding of these processes comes from in vitro monocytic cell culture models as lines or primary isolates where a range and spectra of IL-1 secretion mechanisms have been described. We therefore investigated whether zebrafish embryos provide a suitable in vivo model for studying IL-1 mediated inflammation. Structurally, zebrafish IL-1\u03b2 shares a beta-sheet rich trefoil structure with its human counterpart. Functionally, leukocyte expression of IL-1\u03b2 was detectable only following injury, which activated leukocytes throughout zebrafish embryos. Migration of macrophages and neutrophils was attenuated by caspase-1 and P2X7 inhibitors, which similarly inhibited the activation of NF-\u03baB at the site of injury. Zebrafish offer a new and versatile model to study the IL-1\u03b2 pathway in inflammatory disease and should offer unique insights into IL-1 biology in vivo.<\/jats:p>","DOI":"10.1242\/dmm.013029","type":"journal-article","created":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T02:45:02Z","timestamp":1383878702000},"source":"Crossref","is-referenced-by-count":45,"title":["Zebrafish tissue injury causes up-regulation of interleukin-1 and caspase dependent amplification of the inflammatory response"],"prefix":"10.1242","author":[{"given":"Nikolay V.","family":"Ogryzko","sequence":"first","affiliation":[{"name":"University of Sheffield, Sheffield, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily E.","family":"Hoggett","sequence":"additional","affiliation":[{"name":"University of Sheffield, Sheffield, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Solaymani-Kohal","sequence":"additional","affiliation":[{"name":"University of Sheffield, Sheffield, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Tazzyman","sequence":"additional","affiliation":[{"name":"University of Sheffield, Sheffield, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J. 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