{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:54:44Z","timestamp":1792713284765,"version":"4.5.0"},"reference-count":65,"publisher":"The Company of Biologists","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The skeleton is a preferred homing site for breast cancer metastasis. To date, treatment options for patients with bone metastases are mostly palliative and the disease is still incurable. Indeed, key mechanisms involved in breast cancer osteotropism are still only partially understood due to the lack of suitable animal models to mimic metastasis of human tumor cells to a human bone microenvironment. In the presented study, we investigate the use of a human tissue-engineered bone construct to develop a humanized xenograft model of breast cancer-induced bone metastasis in a murine host. Primary human osteoblastic cell-seeded melt electrospun scaffolds in combination with recombinant human bone morphogenetic protein 7 were implanted subcutaneously in non-obese diabetic\/severe combined immunodeficient mice. The tissue-engineered constructs led to the formation of a morphologically intact \u2018organ\u2019 bone incorporating a high amount of mineralized tissue, live osteocytes and bone marrow spaces. The newly formed bone was largely humanized, as indicated by the incorporation of human bone cells and human-derived matrix proteins. After intracardiac injection, the dissemination of luciferase-expressing human breast cancer cell lines to the humanized bone ossicles was detected by bioluminescent imaging. Histological analysis revealed the presence of metastases with clear osteolysis in the newly formed bone. Thus, human tissue-engineered bone constructs can be applied efficiently as a target tissue for human breast cancer cells injected into the blood circulation and replicate the osteolytic phenotype associated with breast cancer-induced bone lesions. In conclusion, we have developed an appropriate model for investigation of species-specific mechanisms of human breast cancer-related bone metastasis in vivo.<\/jats:p>","DOI":"10.1242\/dmm.014076","type":"journal-article","created":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T11:47:36Z","timestamp":1391773656000},"page":"299-309","source":"Crossref","is-referenced-by-count":120,"title":["A tissue-engineered humanized xenograft model of human breast cancer metastasis to bone"],"prefix":"10.1242","volume":"7","author":[{"given":"Laure","family":"Thibaudeau","sequence":"first","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna V.","family":"Taubenberger","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."},{"name":"Biotec TU Dresden, Tatzberg 47\/49, 01307 Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris M.","family":"Holzapfel","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."},{"name":"Orthopedic Center for Musculoskeletal Research, University of Wuerzburg, Brettreichstrasse 11, 97072 Wuerzburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verena M.","family":"Quent","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Universitaetsstrasse 21\u201323, 91054 Erlangen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Fuehrmann","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."},{"name":"Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Hesami","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby D.","family":"Brown","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Dalton","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl A.","family":"Power","sequence":"additional","affiliation":[{"name":"Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW 2052, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett G.","family":"Hollier","sequence":"additional","affiliation":[{"name":"Australian Prostate Cancer Research Centre, Translational Research Institute, 37 Kent Street, Woolloongabba, Brisbane, QLD 4102, Australia."},{"name":"Tissue Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar W.","family":"Hutmacher","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4049, Australia."},{"name":"Australian Prostate Cancer Research Centre, Translational Research Institute, 37 Kent Street, Woolloongabba, Brisbane, QLD 4102, Australia."},{"name":"George W. 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